کمک به شناسائی برخی درون‌زیهای قارچی مرتبط با زیست‏بوم‏های خشن آبادان و اهواز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی‌ارشد رشته بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 دانشیار قارچ‌شناسی و بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران.

3 استاد قارچ‌شناسی و بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

چکیده
در این پژوهش، 25 نمونه‌ حاوی ریشه، ساقه و برگ گیاهان فاقد علائم متحمل به شوری و گرما در اطراف آبادان و اهواز، جمع‏آوری و قارچ‌های درون‌زی آن‌ها روی محیط غذائی سیب‌زمینی-دکستروز-آگار (PDA) جداسازی شدند. از بین 35 جدایه‌ی ­بدست آمده، تعداد 11 جدایه براساس ریخت‌شناسی ساختارهای جنسی و غیرجنسی جهت شناسایی مولکولی انتخاب شدند. در ارزیابی مولکولی، هویت جدایه‌های انتخابی با استفاده از توالی ژن­های ITS-rDNAو GAPDH(برای جدایه‌های Curvularia) تعیین گردید. بر اساس ترکیبی از داده­های ریخت‌شناسی و مولکولی، جدایه‌های انتخابی به شرح ذیل شناسایی و توصیف شدند؛ Alternaria chlamydospora، A. phragmospora،Chaetomium olivaceum، Curvularia ahvazensis، C. sp.، Fusarium chlamydosporum و Fusarium sp.. بر اساس دانش ما، این اولین گزارش از قارچ‌های درون­زی شناسائی شده ساکن در گونه‌های گیاهیAeluropus littoralis، Atriplex lentiformis، Carthamus oxyacantha، Salicornia subterminalis وScirpus maritimusمی‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Contribution to Identification of Some Fungal Endophytes Associated with the Harsh Ecosystems of Abadan and Ahvaz

نویسندگان [English]

  • Sedigheh Khodadadipour 1
  • Mehdi Mehrabi 2
  • Reza Farokhe nezjad 3
1 1Former MSc Student of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Associate Professor of Mycology and Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
3 Professor of Mycology and Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

Abstract
In this study, 25 samples containing root, stem and leaf of the asymptomatic, salt- and heat-tolerant plants growing around Abadan and Ahvaz, were collected and their endophytic fungi were isolated on potato-dextrose-agar (PDA). Thirty five isolates were obtained, 11 out of them were selected based on the morphology of sexual and asexual structures for molecular identification. In molecular assay, the identity of representative isolates was determined using the sequence data of ITS-rDNA and GAPDH (for Curvularia isolates). Based on a combination of morphological and molecular data, the representative isolates were identified and described as following; Alternaria chlamydospora, A. phragmospora, Chaetomium olivaceum, Curvularia ahvazensis, Curvularia sp., Fusarium chlamydosporum and Fusarium sp.To the best of our knowledge, these fungal endophytes are here reported from Aeluropus littoralis, Atriplex lentiformis, Carthamus oxyacantha, Salicornia subterminalis and Scirpus maritimus for the first time.
 

کلیدواژه‌ها [English]

  • Keywords: Fungal endophytes
  • Salt-tolerant plants
  • Morphological and molecular identification
  • Khuzestan Province
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