شناسایی قارچ‌های مرتبط با بیماری شانکر و سرخشکیدگی درختان نارون در کلان‌شهر تبریز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد بیماری شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه تبریز.

2 استاد، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه تبریز.

چکیده

چکیده
نارون (Ulmus spp.) از جمله مهمترین درختان زینتی در طراحی فضای سبز شهرها به شمار می‌رود. بیماری سرخشکیدگی و شانکر نارون طی سال‌های اخیر در فضای سبز تبریز و سایر شهرهای استان به طور گسترده شیوع پیدا کرده ‌است. در تحقیق حاضر، به منظور شناسایی قارچ‌های همراه با سرخشکیدگی و شانکر درختان نارون در شهر تبریز، طی سال‌های 1394 و 1395 از درختان دارای علائم و سالم نمونه‌برداری انجام‌ گرفت و تعداد 208 جدایه‌ی قارچی از 64 نمونه گیاهی، خالص‌سازی شد. شناسایی گونه‌ها بر اساس داده‌های ریخت‌شناختی و داده‌های توالی ناحیه ITS یا TEF (در مورد برخی گونه‌ها)، صورت ‌پذیرفت. در این بررسی، 16 گونه‌ی قارچی شاملOphiostomanovo-ulmi ، Fusarium solani، F. proliferatum، F. tricinctum،Pestalotiopsis brassicae، Cytospora rhodophila، C. chrysosperma، Didymella glomerata،، Chaetomium globosum، Ch. rectanguare، Clonostachys rosea، Seimatosporium lichenicola،Coniolariella macrothecia ، Pleurostomophora ochracea،Sordaria sp.  و Geosmithia sp. از نواحی پوست و چوب قسمت‌های تنه و شاخه‌های اصلی سالم و دارای علائم تغییر رنگ آوندی جداسازی گردید. در بین جدایه‌های شناسایی شده، جدایه‌های متعلق به جنس‌های Fusarium و Chaetomium از بیشترین فراوانی برخوردار بودند. گزارش گونه‌های Ch. globosum، Ch. rectangulare،F. proliferatum، S. lichenicola، P. brassicae، Co. macrothecia، Pl. ochracea، C. rhodophila، Geosmithia sp.،Cl. roseaوD. glomerataاز روی میزبان نارون برای دنیا جدید می‌باشند.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of Fungal Species Associated with Dieback and Canker Disease of Elm Trees in Tabriz Metropolis

نویسندگان [English]

  • Narges Ahmadi 1
  • Mehdi Arzanluo 2
1 Former MSc Student of Plant Pathology and Mycology, Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Professor of Plant Pathology and Mycology, Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
چکیده [English]

Abstract
Elm is one of the most important ornamental trees and is used extensively in design of urban green space. Dieback and canker disease of elm has been widespread in Tabriz green space and other cities in province in recent years. In order to identify the factors involved in dieback and canker of elm trees in Tabriz city, symptomatic and healthy samples were taken and 208 fungal isolates from 64 plant samples were purified. Identification of species was carried out using morphological and sequence data of the ITS or TEF regions for some species. In this study, 16 species of fungi including, Ophiostoma novo-ulmi, Fusarium solani, F. proliferatum, F. tricinctum, Pestalotiopsis brassicae, Cytospora rhodophila, C. chrysosperma, Didymella glomerata, Chaetomium globosum, Ch. rectanguare, Clonostachys rosea, Seimatosporium lichenicola, Coniolariella macrothecia, Pleurostomophora ochracea, Sordaria sp. and Geosmithia sp were isolated from sapwood and wood parts of trunk and main branches with vascular discoloration and healthy trees. Among the identified isolates, Fusarium and Chaetomium were the most abundant taxa. Chaetomium globosum, Ch. rectangulare, F. proliferatum, S. lichenicola, P. brassicae, Co. macrothecia, Pl. ochracea, C. rhodophila, Geosmithia sp., Cl. rosea and D. glomerata are new reports on elm host worldwide.
 

کلیدواژه‌ها [English]

  • Keywords: Elm
  • Dieback
  • Pestalotiopsis
  • Cytospora
  • Fusarium
  • Ophiostoma
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