متیل جاسمونات ومقاومت به بیماری بلایت خوشه ی گندم ایجاد شده به وسیله ی قارچ Fusarium graminearum

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار بیوتکنولوژی گیاهی،گروه زیست شناسی،دانشکده علوم، دانشگاه یزد.

2 دانشجوی سابق کارشناسی ارشدبیماری شناسی گیاهی،گروه گیاهپزشکی،دانشکده کشاورزی،دانشگاه زابل.

3 کارشناس آزمایشگاه، گروه زیست شناسی، دانشکده علوم، دانشگاه یزد.

چکیده

چکیده
در این تحقیق تعاملات بین بیمارگر Fusarium graminearum و گندم  شامل تغییر در میزان فعالیت چند آنزیم آنتی اکسیدانی و تغیر در سطح بیان ژن‏های , PR2 (b,1-3gluconase),  PR3 (chitinase), و PR4 (wheatwin)مورد بررسی قرار گرفت. از رقم گندم تجن، آزمایش در شرایط کشت آزمایشگاهی و گلخانه­ای به صورت فاکتوریل در قالب طرح کاملا تصادفی با چهار تکرار استفاده شد. محلول پاشی اندام هوایی گیاه توسط متیل جاسمونات با غلظت‌های 200، 400 و 600 میلی گرم در میلی لیتر و یک، سه وپنج روز پس از مایه‌زنی با اسپور قارچFusarium graminearum (غلظت 105 اسپور در هر میلی لیتر) انجام گرفت. همچنین اثر بازدارندگی متیل جاسمونات روی رشد پرگنه­ی قارچ در غلظت‎های مختلف بر روی محیط کشت PDA بررسی شد. نتایج نشان داد که میزان تولید فنلکل و آنزیم‎های پراکسیداز، پلی‏فنل اکسیداز، گایاکول پراکسیداز سه روز بعد از الودگی در غلظت 600 میلی­گرم در میلی­لیتر به بالاترین مقدار خود رسیده ولی  بعد از پنج روز کاهش معنی‎داری نشان داد. بررسی بیان ژن‎ها در بازه­ی زمانی 3 روز بعد از آلودگی نشان داد که ژن بتا 1،3 گلوکوناز و کیتیناز به ترتیب بالاترین و پایین ترین سطح میزان بیان را داشته اند. بررسی اثر بازدارندگی رشد قارچ بوسیله­ی متیل جاسمونات نشان داد که این ماده در غلظت 600 میلی گرم در میلی لیتر بیشترین بازدارندگی را دارا می‏باشد. نتایج بدست آمده بیانگر این است که کاربرد خارجی متیل جاسمونات به عنوان القاگر شیمیایی در ایجاد مقاومت گیاه در برابر بیماری بلایت خوشه­ی  گندم می‏تواند در مدیریت مبارزه با ییماری مورد توجه قرار گیرد. 

 

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Methyl Jasmonat and Resistance to Head Blight Disease on Wheat Caused by Fusarium Graminearum

نویسندگان [English]

  • Seydekazem Sabagh 1
  • Marjan Pykani 2
  • Marziyeh Taheri 3
1 Associate Professor of Plant Biotechnology, Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
2 Former MSc Student of Plant Pathology, Department of Plant Protection, University of Zabol, Iran.
3 Laboratory experts, Department of Biology, Faculty of Science, Yazd University, Yazd, Iran.
چکیده [English]

Abstract
In this research, interaction between Fusarium graminearum  and wheat including the change in rate of some anti-oxidant enzyme activity and expression level of PR2 (b,1-3gluconase), PR3 (chitinase) andPR4 (wheatwin) genes was studied. Tajan cultivar was used in greenhouse and experimental condition in a completely randomized blocks design with four repetitions. Spraying of aerial parts of plants by methyl- jasmonat at 200, 400 and 600 ppm concentration was done one, three and five days after inoculation with fungal spore of Fusarium graminearum (105 spore per milliliter).Also, inhibition effect of methyl jasmonat on growth of fungal colony was investigated on PDA culture medium.The results showed that the total phenol production and peroxidase, polyphenol oxidase and guaiacol peroxidase reached to highest level three  days after inoculation at concentration of 600 ppm and reduced significantly 5 days after inoculation. Gene expression analysis at three days after inoculation time interval showed the highest and lowest expression level for b, one-three gluconase and chitnase respectively. Assay of growth inhibition effect by methyjasmonat showed that this material has the highest inhibition activity at concentration of 600 ppm. These results indicate that exogenous application of methyl jasmonat as chemical inducer to induce plant resistance against head scab disease could be considered in plant disease management.
 

کلیدواژه‌ها [English]

  • Keywords: Inducted resistance
  • Pathogenesis related protein
  • Antioxidant enzymes
  • Total Phenol
  • Real time PCR
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