بررسی خصوصیات بیولوژیکی و مولکولی چند جدایه ویروس موزائیک خیارCucumber mosaic virus (CMV))) از مناطق شمال غرب ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه گیاه پزشکی، دانشگاه آزاد اسلامی، واحد تبریز، تبریز.

2 دانشیار، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز.

3 استادیار گروه گیاه پزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی ،تهران.

چکیده

چکیده
     ویروس موزائیک خیار یکی از مهمترین ویروس­های آلوده کننده­ی گیاهی در ایران می­باشد. به منظور بررسی فنوتیپ آلودگی جدایه­های مختلف این ویروس در سال 1390 تعداد 342 نمونه­ی گیاهی مشکوک به آلودگی با این ویروس از گیاهان خیار، کدو، خربزه و گوجه فرنگی از مناطق مختلف شمال غرب کشور جمع­آوری و با آزمون الایزا 91 نمونه­ی آلوده به CMV ردیابی شد. از بین جدایه ها هشت جدایه که توالی ژنومی و موقعیت تبارزایی آن­ها مشخص گردیده بود، برای مقایسه­ی خصوصیات بیولوژیکی و مطالعات بیشتر انتخاب شدند. شاخص بیماری برای این جدایه ها روی کدو محاسبه گردید. براساس علایم، جدایه ها در دو گروه متمایز شدند. بررسی بیان ژن پروتئین پوششی و سرکوب گر خاموشی RNA ((2b با استفاده از تکنیک RT-PCR نیمه کمی، نشان داد که غلظت جدایه­ها در گیاهان محک متفاوت می­باشد. جدایه­های ایرانی زیر گروه IB علائم شدیدتری را بر روی گیاهان محک نسبت به جدایه­ های زیر گروه IA نشان دادند. بنابراین به نظر میرسد که بین ویژگی­های ژنوتیپی و خصوصیات بیماری زایی جدایه ها ارتباط معنی داری وجود دارد.
 

کلیدواژه‌ها

عنوان مقاله [English]

Molecular and Biological Characteristics of Some Iranian Isolates of Cucumber mosaic virus from North West Region of Iran

نویسندگان [English]

  • Sevil Nematollahi 1
  • Nemat Sokhandan Bashir 2
  • Farshad Rakhshandehro 3
  • Hamid Reza Zamanizadeh 3
1 Assistant Professor, Department of Plant Protection, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
2 Associate Professor, Department of Plant Protection, Faculty of Agriculture University of Tabriz,
3 Assistant Professor, Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Abstract
Cucumber mosaic virus (CMV) is one of the most important infectious plant viruses in Iran. For evaluating infection phenotypes of different isolates 342 samples of cucurbitaceous and tomato plants were collected from different locations in the northwest region of Iran in 2012. After screening by enzyme-linked immune sorbent assay (ELISA), 91 CMV-infected samples were identified. Eight representative CMV isolates whose phylogenetic positions had been determined were selected and compared for their pathological features in zucchini (Cucurbita pepo cv. Ps) plants.  According to the determined disease indexes, the isolates were placed in two main groups. Also, evaluation of 2b (viral suppressor of RNA silencing) and CP expression with semi-quantitative RT-PCR showed different concentration of the isolates in test plants. The subgroup IB members from Iran induced more severe symptoms on the inoculated indicator test plants compared to the isolates in subgroup IA. Thus, there seemed to be a correlation between subgroup affiliation and biological properties of the CMV isolates from Iran. 
 

کلیدواژه‌ها [English]

  • Keywords: CMV
  • Phylogenetic
  • Subgroup
  • Phenotype

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پژوهش های کاربردی در گیاهپزشکی
دوره 6، شماره 3
آذر 1396
صفحه 97-108

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سابقه مقاله

  • تاریخ دریافت: 02 دی 1396
  • تاریخ پذیرش: 02 دی 1396

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