کارایی نماتد بیمارگر حشرات Heterorhabditis bacteriophora در کنترل زیستی نماتد گره ریشه Meloidogyne javanica در شرایط آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد بیماری شناسیگیاهی، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز

2 دانشیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز

3 استاد بهنژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز

چکیده

در سال‌های اخیر، کنترل زیستی نماتدهای بیمارگر گیاهی با استفاده از نماتدهای بیمارگر حشرات مورد توجه قرار گرفته است. این تحقیق تاثیر لاروهای آلوده‌کننده زنده و مرده نماتد بیمارگر حشرات Heterorhabditis bacteriophora Poinar در غلظت‌های 5، 10، 25، 50 و 100 لارو در هر میلی‌لیتر را به‌طور جداگانه و در ظرف‌های 24 چاهکی روی مرگ ومیر لارو وتفریخ تخم نماتد گره ریشه Meloidogyne javanica Treub در غلظت‌های 50 تخم، 50 لارو و مخلوط 50 تخم و لارو به ازای هر چاهک در شرایط آزمایشگاهی مورد بررسی قرار داد. آزمایش با شش تیمار در قالب طرح کرت‌های خرد‌شده در زمان و چهار تکرار برای هر تیمار انجام شد. کل آزمایش‌ها دو بار تکرار گردید. یادداشت‌‌برداری نتایج بعد از 48، 72، 96 و120 ساعت نشان داد که تاثیر لارو زنده یا مرده نماتد بیمارگر حشرات در مرگ‌ومیر لاروهای سن دوم و تفریخ تخم نماتد گره ریشه معنی‌دار است. بیش‌ترین مرگ‌ومیر در غلظت100 لارو آلوده‌کننده در هر میلی‌لیتر و در 120ساعت و بیش‌ترین بازدارندگی از تفریخ تخم با غلظت 100 لارو آلوده‌کننده در هر میلی‌لیتر و در 48 ساعت مشاهده شد. در حالت مخلوط 50 تخم و لارو نماتد گره ریشه، تاثیر لاروهای زنده H. bacteriophora در مرگ‌ومیر لارو نماتد گره ریشه در بین زمان‌ها اختلاف معنی‌داری بروز نداد.

کلیدواژه‌ها

عنوان مقاله [English]

Efficacy of Entomopathogenic Nematode Heterorhabditis bacteriophora asBiological Control of Root Knot Nematode Meloidogyne javanicaUnder Laboratory Conditions

نویسندگان [English]

  • Rahimeh Ghaffarpoor Teimurloii 1
  • Gholam Reza Niknam 2
  • Mahmoud Toorchi 3
1 Former MSc. Student of Plant Pathology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Associate Professor, Dept of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 Professor, Dept of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
چکیده [English]

Antagonism of entomopathogenic nematodes (EPNs) on plant – parasitic nematodes (PPNs) has gained interest over the recent years. Therefore, the efficacy of live and dead infective juveniles of entomopathogenic nematode, Heterorhabditis bacteriophora Poinarwith concentrations of 5, 10, 25, 50 and 100 IJs/ml in 24 well plates was evaluated on egg hatching inhibition and second stage juveniles (J2) mortality of the root knot nematode Meloidogyne javanica Treub in concentrations of 50 eggs, 50 larvae and a combination of 50 eggs and larvae. The experimental design was split plot with six treatments and four replications. The whole experiments were replicated twice. The data recorded after 48, 72, 96 and 120 hours showed that both live and dead entomopathogenic nematodes have significant difference on root knot second stage juveniles (J2) mortality and egg hatching inhibition. The highest mortality was achieved in 100 IJs/ml and after 120 hours and the most inhibition of hatching in concentration of 100 IJs/ml and after 48 hours. Concerning the mixture of 50 eggs and larvae, the results indicated that the effect of live juveniles of H. bacteriophora on second stage juveniles (J2) mortality have no significant difference among the various time treatments.

کلیدواژه‌ها [English]

  • Entomopathogenic nematodes
  • Root knot nematode
  • Heterorhabditis bacteriophora
  • Meloidogyne javanica

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سابقه مقاله

  • تاریخ دریافت: 06 اسفند 1391
  • تاریخ بازنگری: 01 بهمن 1392
  • تاریخ پذیرش: 24 شهریور 1392

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