کارایی سولفیت پتاسیم در مقایسه با ترکیبات زیستی و شیمیایی علیه نماتد مولد زخم ریشه‌ چای (Pratylenchus loosi)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران

2 پژوهشکده چای، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، لاهیجان، ایران

چکیده

نماتد زخم ریشه‌ چای (Pratylenchus loosi)، یکی از عوامل خسارت‌زا در جهان و ایران بوده و باعث خسارت کمی و کیفی به این محصول می‌شود. در این تحقیق، کارایی سولفیت پتاسیم به میزان پنج، 10 و 15 گرم و نماتد­کش­ های شیمیایی و آلی فلوپیرام (ولوم) به میزان نیم، یک و یک و نیم میلی ­لیتر، کادوزافوس (راگبی) به میزان هفت و نیم گرم، کود زیستی نماکلین به میزان پنج، 15 و 20 میلی ­لیتر برای هر نهال جوان چای همراه با شاهد­های سالم و آلوده برای کنترل نماتد­ مولد زخم ریشه‌ چای مورد ارزیابی قرار گرفت. آزمون در قالب طرح بلوک‌های کامل تصادفی با 12 تیمار و پنج تکرار در ایستگاه تحقیقات چای شهید افتخاری شفت (فشالم) در غرب استان گیلان اجرا شد. شاخص‌های قطر، ارتفاع و وزن نهال، حجم ریشه، وزن تر ریشه، وزن تر و خشک اندام هوایی، جمعیت نماتد در 100 گرم خاک و یک گرم ریشه اندازه‌گیری و ثبت گردید. نتایج نشان داد که تیمار راگبی در دو شاخص حجم ریشه و وزن تر ریشه؛ تیمار ولوم یک، در شاخص وزن تر اندام هوایی؛ ولوم یک و نیم و سولفیت پتاسیم پنج، در شاخص وزن خشک اندام هوایی؛ تیمار ولوم یک و نیم در دو شاخص جمعیت نماتد در خاک و ریشه بهترین اثر بخشی را داشتند. نتایج حاصل از این تحقیق نشان داد که دو ترکیب راگبی و ولوم، از بیشترین تأثیر در شاخص‌ها برخوردار بودند. ترکیب‌های سولفیت پتاسیم و نماکلین از نظر کاهش جمعیت در خاک و ریشه می‌توانند به‌عنوان گزینه ­ای در مدیریت تلفیقی نماتد مولد زخم ریشه‌ چای مورد توجه واقع شوند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of potassium sulfite compared with biological and chemical components against tea root lesion nematode (Pratylenchus loosi)

نویسندگان [English]

  • Samira Mehrzad Salakjani 1
  • Salar Jamali 1
  • Ali Seraji 2
  • Sanam Safaei Chaeikar 2
1 Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran
2 Tea Research Center, HSRI, AREEO, Lahijan, Iran
چکیده [English]

Root lesion nematode, Pratylenchus loosi is one of the important damaging agent of tea crop in world and Iran which, causing quantitative and qualitative damage to this crop. In this research, the effectiveness of Potassium Sulfite at the rate of 5, 10 and 15 g and chemical and organic nematicide of Flopiram (Velum) at 0.5, 1, and 1.5 ml, Cadusafos (Rugby) at 7.5 g,  Nemaclean biofertilizer at 5, 15, and 20 ml for each young tea seedling along with healthy and infected control plants against root lesion nematode were evaluated. The experiment was conducted in a completely randomized block design with 12 treatments and 5 repetitions at Shaft Shahid Eftekhari tea research station (Fashalam) in the west of Guilan province. The studied indices were diameter, height, and weight, root mass, root fresh weight, shoot fresh and dry weight of seedling, nematode population in 100 g of soil, and in one gram of root measured and recorded, too. Results showed that Rugby treatment, in two indices of root mass and fresh root weight, Velum 1 treatment, in shoot fresh weight, Velum 1.5 and Potassium Sulfite 5, in shoot dry weight, Velum 1.5 in two nematode population indices in soil and roots, had the best effect. Results of this study revealed that the Rugby and Velum components had the greatest impact on performance indices. Potassium sulfite and Nemaclean compounds in terms of population reduction in the soil and roots, can be considered as a safe alternative in the integrated management of tea root lesion nematode.

کلیدواژه‌ها [English]

  • Management
  • Nemaclean
  • Potassium Sulfite
  • Tea
  • Velum
Alesaadi GhA, Moosavi SMR, Basirnia T, 2017. Effect of nano-k, potassium sulphate and salicylic acid on tomato growth and control of root-knot nematode (Meloidogyne javanica). Plant protection (Scientific Journal of Agriculture) 40(3): 71–82.
Banihashemian SN, Jamali S, Golmohammadi M, Ghasemnezhad M, 2022. Isolation and identification of endophytic bacteria associated with kiwifruit and their biocontrol potential against Meloidogyne incognita. Egyptian Journal of Biological Pest Control 32(1): 111.
Banihashemian SN, Jamali S, Golmohammadi M, Ghasemnejad M, 2023a. Specific identification of root-knot nematode Meloidogyne incognita common in kiwifruit orchards of Guilan and Mazandaran provinces using sec-1 gene sequence. Journal of Applied Research in Plant Protection 11(4): 63–72.
Banihashemian SN, Jamali S, Golmohammadi M, Noorizadeh S, Atighi MR, 2023b. Reaction of Commercial Cultivars of Kiwifruit to Infection by Root-knot Nematode and Its Biocontrol Using Endophytic Bacteria. Journal of Nematology 55(1): 1–10.
Banihashemian SN, Jamali S, Golmohammadi M, Ghasemnezhad M, 2023c. Management of root-knot nematode in kiwifruit using resistance-inducing Bacillus altitudinis. Tropical Plant Pathology 48(4): 443–451.
Campos VP, Sivapalan P, Gnanapragasam NC, 1990. Nematodes parasites of coffee, cocoa and tea. In: Luc M, Sikora RA, Bridge J (eds). Plant parasitic nematodes in subtropical and tropical agriculture. CAB International, London. Pp. 404-430.
Charehgani H, Karegar Bideh A, Hamzehzarghani H, 2010. Effect of chemical fertilizers on root-knot nematode (Meloidogyne incognita) in greenhouse cucumber cultivation. Iranian Journal of Plant Pathology 46(3): 263–274 (In Persian with English abstract).
Coolen WA, D’ Herde CJ, 1972. A method for the quantitative extraction of nematodes from plant tissue. State Nematology and Entomology Research Station, Ghent Agriculture, 77 pp.
Faske TR, Hurd K, 2015. Sensitivity of Meloidogyne incognita and Rotylenchulus reniformis to fluopyram. Journal of Nematology 47: 316–21.
Farahat A, Al-Sayed A, Adam M, Diab S, 2018. Comparative efficacy of biotic and abiotic Agro-xommercial products pgainst Rotylenchulus reniformis under field conditions. Egyptian Journal of Agronematology 17(1): 77–93.
Forghani F, Hajihassani A, 2020. Recent advances in the development of environmentally benign treatments to control root-knot nematodes. Frontiers in Plant Science 11: 1125.
Kumhar KC, Babu A, Nisha SN, Kishor CK, 2022. Management of tea (Camellia sinensis) diseases with application of microbes: a review. Applied Microbiological Biotechnology 10: 2022.
Mirghasemi N, Fanelli E, Jamali S, Sohani MM, De Luca F, 2021 Molecular Characterization of Three B-1,4-Endoglucanase Genes in Pratylenchus loosi and Functional Analysis of Pl-eng-2 Gene. Plants 10: 2022.
Mahdavian A, Eshtiaghi H, Barouti Sh, Mojdehi H, 2000. Effective method of controlling Meloidogyne. incognita nematode in Guilan tobacco fields in greenhouse. Proceedings of 14th Iranian Plant Protection Congress, August 29–31, Isfahan, Iran. p. 120. (in Persian with English abstract).
Meza P, Elgueta S, Sagredo B, 2021. Evaluation of fluopyram for management of Meloidogyne ethiopica and migratory nematodes in commercial tomato greenhouses in the Metropolitan Region of Chile. Pest Management Science 77(6): 2863–2869.
Mohammad Alian Y, Banihashemian SN, Golmohammadi M, Banihashemian SM, Bashiri S, 2018. Evaluation of the tolerance of some citrus rootstocks to citrus nematode in greenhouse (Tylenchulus semipenetrans). Iranian Plant Protection Research 31(4): 700–705 (In Persian with English abstract).
Mohotti KM, Prematunga AK, Herath UB, Amarasena PGDS, 2018. New prophylactic nematicide for integrated nematode management in tea. 235th Experiments and Extension Forum, New Pesticides for Integrated Pest Management in Tea. Talawakelle, Sri Lanka. P. 10.
Safdar H, Javed N, Khan SA, Ul Haq I, Safdar A, et al., 2012. Control of Meloidogyne incognita (Kofoid and White) chitwood by cadusafos (Rugby®) on tomato. Pakistan journal of Zoology 44(6): 1703–1710.
Sang H, Witte A, Jacobs JL, Chang HX, Wang J, et al., 2018. Fluopyram sensitivity and functional characterization of SDHB in the Fusarium solani species complex causing soybean sudden death syndrome. Frontiers in Microbiology 9: 2335.
Santana-Gomes SMS, Arieira DRC, Roldi M, Dadazio ST, Marini MP, et al., 2013. Mineral nutrition in the control of nematodes. African Journal of Agricultural Research 8: 2413–2420.
Seifi S, Karegarbideh A, 2013. Effect of mineral fertilizers on cereal cyst nematode Heterodera filipjevi population and evaluation of wheat. World Applied Programming 3: 137–141.
Seraji A, 2007. Biological and dynamic study of Pratylenchus loosi nematode population in Iran and the possibility of assessing its damage using epidemiological models. Tarbiat Modares University, Tehran, 208 P.
Seraji A, Pourjam E, Tanhamaafi Z, Safaei N, 2009. The economic threshold determining of tea root lesion nematode in Iran. Proceedings of 6th Horticultural Sciences of Iran Congress, July 13, Rasht, Iran. p. 690. (in Persian with English abstract).
Seraji A, Mir Ghasemi ST, 2010. Forest millet weed Oplismenus compositus is the new host of tea root wound nematode. Journal of Plant Diseases 46(1): 91–92.
Seraji A, Pourjam E, Safaie N, Tanhamaafi Z, 2012. Effect of different population of root lesion nematode, Pratylenchus loosi, on growth tea saplings. Proceedings of 20th Iranian Plant Protection Congress, Shiraz, Iran. p. 641. (in Persian with English abstract).
Sivapalan P, 1967. Nematodes and tea. Tea Quarterly 38(2): 178–185.
Tanhamaafi Z, 1992. Report of rootworm nematode of Pratylenchus loosi on tea seedlings imported from Japan. Journal of Plant Pests & Diseases 60: 94–93.
Tanhamaafi Z, Damadzadeh M, 1995. An investigation on the effect of nonvolatile nematicides on citrus nematode in north of Iran. 12th Iranian Plant Protection Congress, Karaj, Iran. p. 228. (in Persian with English abstract).
Tanhamaafi Z, Mir Hosseini Moqaddam SA, Gholamreza Zadeh Yousefi N, Farid R, 2002. Use of nematicides and fertilizers in controlling tea root lesion nematode in Guilan province. Final Report of Research Projects of the General Directorate of Tea Research Services, 28 pp.
Touray M, Cimen H, Gulsen SH, Ulug D, Erdogus D, et al., 2021. The impact of chemical nematicides on entomopathogenic nematode survival and infectivity. Journal of Nematology 53(1): 1–17.
Vawa OST, Gnonhouri GP, Seri SP, Adiko A, Otchoumou A, 2019. Evaluation de l’efficacite d’une formulation de fluopyram (velum prime) contre deux nematodes Radopholus similis et Pratylenchus coffeae, en culture de bananier en Cote d’ivoire. Agronomie Africaine 8(1): 161–166.
Whitehead AG, Hemming JR, 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology 55(1): 25–38.‏
Zheng Y, Duan Y, Chen S, Sun J, Chen L, 2010. Responses of soybean cyst nematode Heterodera glycines to macroelement and microelement compounds. Bulgarian Journal of Agricultural Science 16: 172–180