Genetic diversity of seven-spotted ladybug populations in Iran using cytochrome oxidase gene analysis

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 گروه تاکسونومی حشرات، موسسه تحقیقات گیاهپزشکی کشور، تهران

چکیده

Lady beetles, particularly the seven-spotted lady beetle, play an essential role as biological control agents against aphids and other pests in agricultural ecosystems. Studying their molecular biology can provide insights into their adaptation, behavior, and potential for pest control. Recent progress in genomic technologies has led to a better understanding of the molecular mechanisms involved in these processes. In this study, we examined the genetic structure of Iranian populations of this species by sequencing the cytochrome oxidase gene. Analysis of thirty-three genetic sequences (positions 1-576) showed a haplotype diversity of 0.945, with 25 distinct haplotypes identified. The nucleotide diversity was relatively low (π = 0.00658), revealing a discrepancy between haplotype diversity and nucleotide diversity. This pattern suggests the presence of a genetically diverse population with conserved genomic regions, likely resulting from recent population expansion or selective pressures. Our findings provide important information about population dynamics and offer valuable insights for conservation strategies and ecological planning.

کلیدواژه‌ها

عنوان مقاله [English]

تنوع‌ژنتیکی جمعیت‌های کفشدوزک هفت‌نقطه‌ای در ایران با استفاده از ژن سیتوکروم اکسیداز

نویسندگان [English]

  • fatemeh sadat hosseini 1
  • Majid Kazzazi 1
  • fatemeh abdolahadi 2
1 Department of Plant Protection, School of Agriculture, Buali sina University, Hamedan, Iran
2 Department of Insect Taxonomy, Iranian Research Institute of Plant Protection Research, Tehran
چکیده [English]

کفشدوزک‌ها، به ویژه کفشدوزک هفت‌نقطه‌ای، نقش حیاتی در اکوسیستم‌های کشاورزی به عنوان عوامل کنترل بیولوژیک شته و آفات دیگر ایفا می‌کنند. درک زیست‌شناسی مولکولی آن‌ها می‌تواند بینشی درباره سازگاری، رفتار و پتانسیل کنترل آفات ارائه دهد. پیشرفت‌های اخیر در فن‌آوری‌های ژنومی، درک عمیق‌تری از مکانیزم‌های مولکولی زیربنایی این فرآیندها را فراهم کرده است. این مطالعه با هدف بررسی ساختار ژنتیکی جمعیت‌های این گونه در ایران، از طریق توالی‌یابی ژن سیتوکروم اکسیداز انجام شد. نتایج تحلیل سی‌و‌سه توالی ژنتیکی (نواحی 576–1)، مقدار تنوع ژنتیکی در سطح هاپلوتیپ‌ها را برابر با  (H =0.945) نشان داد. بیست‌و‌پنج هاپلوتیپ متمایز شناسایی گردید. در عین حال، میزان تنوع نوکلئوتیدی (π =0.00658)  نسبتاً پایین به دست آمد که نشان‌دهنده اختلاف بین تنوع در سطح هاپلوتیپ و تنوع در سطح نوکلئوتید است. این تناقض نشان‌دهنده یک ذخیره ژنتیکی متنوع با نواحی حفاظت‌شده ژنومی است که احتمالاً به‌دلیل گسترش جمعیتی اخیر یا تأثیر فشارهای انتخابی به وجود آمده است. نتایج این بررسی، اطلاعات ارزشمندی در رابطه با دینامیک جمعیتی، راهبردهای حفاظت و برنامه‌ریزی مطالعات زیست‌محیطی فراهم می‌کند.

کلیدواژه‌ها [English]

  • تنوع نوکلئوتیدی
  • ژنتیک جمعیت
  • مدیریت اکولوژیک
  • هاپلوتیپ

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پژوهش های کاربردی در گیاهپزشکی
دوره 14، شماره 1
فروردین 1404
صفحه 57-69

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  • تاریخ دریافت: 07 بهمن 1403
  • تاریخ بازنگری: 18 اردیبهشت 1404
  • تاریخ پذیرش: 21 اردیبهشت 1404

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