نوع مقاله : مقاله پژوهشی
نویسندگان
مؤسسه تحقیقات ثبت و گواهی بذر ونهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Seed-borne diseases have important effects on diseases epidemic and emergencing of new diseases. The present study, in addition to monitoring the contamination of seed samples of different bean cultivars which harvested from different regions of the Iran from 2022 to 2024, investigated the transmission of two seed-borne bacteria Xanthomonas phaseoli pv. phaseoli (Xpp) and Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) in daughter plants and also in the next generation field. Disease surveys were done in different growth stages via visual field evaluation. The pathogenes were detected in some of seed samples using culture method and specific PCR. The highest rate of infection with both disease agents was recorded in Yaghout and Dadfar cultivars from Lorestan and Markazi provinces, respectively. Investigation of the pathogens transmission from seed to plant under controlled greenhouse conditions by using specific PCR with primers p7X4c, p7X4c (for Xpp) and CffFOR2, CffREV4 (for Cff), showed the epiphytic and endophytic development of Xpp and Cff in different tissues of the plant including root, leaf, stem and seed. Xpp and Cff have successful establishment in the vascular tissue of host plant and cause developed systemic infection. Study of the disease transmission rate in the natural conditions in the field showed, only less than 50% of infected seeds produced infected plants. Despite the complete transmission of the disease in the controlled conditions in the greenhouse, the transmission rate in the field may be depends on several factors including weather conditions, bacterial population in the seed and plant resistance which needs more studies.
کلیدواژهها [English]
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