نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آباد، ایران.
2 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، . ایران.
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Saffron (Crocus sativus L), is one of the most valuable medicinal plants that can be propagated only vegetatively by its corm. Various soil-borne pathogens, especially Fusarium oxysporum, the causal agent of corm rot, seriously damage saffron cultivation worldwide. Transcription factors (TFs) such as Helix Loop Helix (bHLH), regulate the expression of many genes including those involved in resistance against pathogens. In this study, the transcriptome of saffron corms infected with F. oxysporum was evaluated 48 and 72 hours post-inoculation. Arabidopsis bHLH sequences were used to identify the bHLH members in saffron transcriptomic data. Finally, the expression pattern of the candidate bHLH genes was assessed using the Real-Time qRT-PCR method under biological stress and control. The results of this study led to the identification of 50 bHLH encoding sequences in the transcriptome of saffron corms. Analysis of the expression of selected bHLH genes showed that the highest and the lowest expression levels were found in the lateral bud and root tissues, respectively. Additionally, the expression analysis of candidate genes from the bHLH family in response to corm rot disease revealed a pattern consistent with the RNA-seq data. According to the results of the present study, corm rot disease in saffron increased the expression of bHLH genes, suggesting that bHLH genes are involved in resistance against corm rot disease in saffron. Thus, the findings of this study may help in identify key effective bHLH transcription factors involved in responding to biotic stresses in saffron. Furthermore, the results of present study can be applied in saffron future breeding programs.
کلیدواژه‌ها [English]
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