نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشگاه ولی عصر(عج) رفسنجان. بیماری شناسی گیاهی، دانشگاه ولی عصر(عج) رفسنجان، رفسنجان، ایران.
2 گروه ژنتیک و تولیدات گیاهی، دانشگاه ولی عصر(عج) رفسنجان. رفسنجان، ایران.
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is one of the most important diseases of common bean that threatens the dry bean industry worldwide. The management of this disease especially its biological control remains as a serious problem. The use of plant growth promoting rhizobacteria (PGPR) is a proven environment friendly way of controlling plant disease and increasing crop yield. The purpose of this study is to isolate and identify symbiotic and non-symbiotic rhizobacteria against bacterial wilt of common bean and to evaluate the beneficial characteristics of bacterial isolates. A total of 100 bacterial isolates were isolated from the rhizosphere and root nodules of bean, soybean, lentil, chickpea, fenugreek, yellow alfalfa and alhagi. Strains were screened for their antagonistic activity such as inhibitory halo and plant growth-promoting characteristics including production of either, solubilization lipase and hydrogen cyanide and solubilization of phosphate. Eleven isolates were selected and their ability to induce resistance in bean plants (cv. Khomein) against Cff was tested in greenhouse conditions. Morewer, defense enzymes such as peroxidase, polyphenol oxidase, phenylalanine ammonialyase, and total phenols contents were determined in different treatments. The results showed that the level of these enzymes increased in the combination of rhizobium and pathogen. Analysis of the16SrRNA gene sequences revealed that representative isolates belongs to the genera Pantoea, Sinorhizobium, Enterobacter, Xanthomonas, and Rhizobium. Our research revealed that the isolated rhizobacteria strains exhibit both potential for biological nitrogen fixation and antagonistic activity.Given the constraints associated with chemical compounds, the employment of these bacteria as a viable solution in the management of bacterial wilt of bean appears crucial and essential for a safe, efficient, and sustainable approach.
کلیدواژه‌ها [English]
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