بررسی امکان مهار زیستی پژمردگی باکتریایی لوبیا ناشی ازCurtobacterium flaccumfaciens pv. flaccumfaciens با استفاده از ریزوباکترهای همزیست و غیرهمزیست

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشگاه ولی عصر(عج) رفسنجان. بیماری شناسی گیاهی، دانشگاه ولی عصر(عج) رفسنجان، رفسنجان، ایران.

2 گروه ژنتیک و تولیدات گیاهی، دانشگاه ولی عصر(عج) رفسنجان. رفسنجان، ایران.

چکیده

پژمردگی باکتریایی با عامل Curtobacterium flaccumfaciens pv. flaccumfaciens از مهم‌ترین بیماری‌های لوبیای معمولی است. پژوهش حاضر با هدف ارزیابی امکان کنترل زیستی این بیماری با استفاده از ریزوباکترهای همزیست و غیرهمزیست انجام گرفت. در مجموع 100 جدایه باکتریایی از گیاهان لوبیا، سویا، عدس، نخود، شنبلیله، یونجه زرد و خارشتر جداسازی شدند و ویژگی‌های آنتاگونیستی در آن‌ها مورد ارزیابی قرار گرفت. یازده جدایه دارای ویژگی مناسب انتخاب شدند و توانایی آن‌ها در القای مقاومت روی گیاهان لوبیای محلی خمین در برابرCff  در شرایط گلخانه مورد بررسی قرار گرفت. علاوه بر آن، سطح آنزیم‌های دفاعی پراکسیداز، پلی فنل اکسیداز، فنیل آلانین آمونیالیاز و محتوای فنل کل در تیمارهای مختلف مورد سنجش و ارزیابی قرار گرفت و مشخص شد که سطح این آنزیم‌ها در تیمارهای ترکیب رایزوبیوم و بیمارگر افزایش یافته است. نتایج آزمون‌های بیوشیمیایی و آنالیز ترادف نوکلئوتیدی ناحیه 16S rRNA نشان داد که یازده جدایه برتر متعلق به جنس‌های Sinorhizobium، Pantoea، Xanthomonas، Enterobacter وRhizobium   می‌باشند. یافته‌های ما نشان داد که تعدادی از این ریزوباکترها علاوه بر توانایی بالقوه در تثبیت بیولوژیکی نیتروژن، دارای توان آنتاگونیستی نیز می‌باشند. بنابراین استفاده از این باکتری‌ها می‌تواند به عنوان جایگزینی ایمن، مؤثر و پایدار در سیستم‌های کشاورزی مورد توجه باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Biocontrol of bacterial wilt of bean (Curtobacterium flaccumfaciens pv. flaccumfaciens) using symbiotic and non-symbiotic rhizobacteria

نویسندگان [English]

  • Sedighe Mohammadikhah 1
  • Pejman Khodaygan 1
  • Khalil Malekzadeh 2
  • Roohallah Saberi Riseh 1
1 Department of Plant Protection, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran.
2 Department of Genetics and Plant Production, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran.
چکیده [English]

Bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is one of the most important diseases of common bean. The purpose of this study was to isolate and identify symbiotic and non-symbiotic rhizobacteria with antagonisitic potential against bacterial wilt of common bean and to evaluate the beneficial characteristics of bacterial isolates. A total of 100 bacterial strains were isolated from bean, soybean, lentil, chickpea, fenugreek, yellow alfalfa and alhagi. Strains were screened for their antagonistic activity. Eleven isolates were selected and their ability to induce resistance in bean plants (cv. Khomein) against Cff was tested under greenhouse conditions. Morewer, defense enzymes such as peroxidase, polyphenol oxidase, phenylalanine ammonialyase, and total phenols contents were determined in different treatments. The results showed that the level of these enzymes increased in the combination of rhizobium and pathogen. Analysis of the 16SrRNA gene sequences revealed that representative isolates belong to the genera Pantoea, Sinorhizobium, Enterobacter, Xanthomonas, and Rhizobium. Our research revealed that the isolated rhizobacteria strains exhibit both potential for biological nitrogen fixation and antagonistic activity. Given the constraints associated with chemical compounds, the employment of these bacteria as a viable solution in the management of bacterial wilt of bean appears crucial and essential for a safe, efficient, and sustainable approach.

کلیدواژه‌ها [English]

  • Bacterial Wilt of Bean
  • Biocontrol
  • Induced systemic resistanc Growth-promoting bacteria
  • Rhizobium
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