نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرمآباد، ایران.
2 روه گیاهپزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران.
3 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه بوعلی سینا همدان، همدان، ایران.
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Trichoderma species are among the most abundant soil-inhabiting fungi with global distribution, occuring in wide range of climates. While most species are cosmopolitan, some species are restricted to certain geographical regions. Members of this genus have been evaluated as a potential plant disease biocontrol agents (BCA) and plant growth promoters. This study was conducted for the identification of Trichoderma species from the forest areas of the Khorramabad County, Iran. Soil samples were collected from different geographical areas in the Khorramabad County during different seasons of 2019-2020. After isolation and purification as well as screening of isolates based on morphological characteristics, isolates with different morphological characteristics were selected for molecular studies. A total of 200 isolates belonging to three sections namely Trichoderma, Pachybasium and Longibrachiatum including eight species namely, T. atroviride, T. brevicompactum, T. citrinoviride, T. longibrachiatum, T. virens, T. viridescens, Trichoderma sp. 1 and Trichoderma sp. 2 were identified based on their morphology and phylogenetic analysis of ITS region and tef1-a gene. The results showed the that two isolates namely Trichoderma sp. 1 of Trichoderma section and Trichoderma sp. 2 of Longibrachiatum section were not similar to any of the described Trichoderma species in terms of micromorphological characteristics, thus representing candidates for novel Trichoderma species.
کلیدواژه‌ها [English]
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