نوع مقاله : مقاله پژوهشی
نویسندگان
گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Potato Solanum tuberosum is an economically significant crop with high nutritional value, which annually is adversely affected by fungal diseases, resulting in economic yield losses. In the present study, 25 fungal isolates with similar colony morphology and growth pattern were isolated from among 30 potato tuber samples with symptoms of potato black scab (Rhizoctonia solani) collected from the Sarab region, East Azarbaijan province. Identification of fungal strains was carried out using a combination of morphological and molecular data from the ITS-rDNA region and the beta-tubulin gene (tub2). The inferred phylogenies based on the combined dataset of these two gene regions placed the examined strains together with sequence data of the type specimen of Trichocladium solani in the family Chaetomiaceae. The fungal strains cultured on oatmeal agar (OA) media produced simple, aseptate, acromonilioid conidiophores formed laterally on unbranched, transparent hyphae. The morphological characteristics of the examined strains were fully consistent with the description provided for the species T. solani. Pathogenicity assay on potato tubers under laboratory conditions confirmed T. solani isolates being pathogenic on potato. Trichocladium solani was recently described and reported as a new species causing yellow rot disease on potato tuber rot in Russia. In this study, T. solani is reported for the first time from Iran, and the morphological, pathological, and molecular characteristics of this fungus are determined. Geographical distribution, host range and economic impact of the the disease remain to be studied.
کلیدواژهها [English]
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