نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه گیاهپزشکی، دانشکده کشاورزی دانشگاه لرستان،لرستان، خرم آباد، ایران
2 گروه گیاهپزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه لرستان، خرمآباد، ایران.
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Monilinia spp. cause brown rot disease on members of the of the Rosaceae family around the world. This study was conducted to identify Monilinia species causing brown rot disease on apple trees in the in Khorramabad region by the integration of morphological and molecular methods and biocontrol of this disease using antagonistic bacteria under storage conditions.Towards this aim, apple orchards in the Khoramabad region were inspected and samples were collected from blossom blight and fruit rot rot symptoms. Fungal isolates were recovered using routine plant pathology techniques. Based on the integration of morphological data and PCR amplification results using species-specific primers, all of the isolates were identified as M. laxa. The biological control properties of four antagonistic bacteria Serratia marcescens, Bacillus cereus, Bacillus sp and Pseudomonas baetica were tested in laboratory and storage conditions against M. laxa causing brown rot of apple fruits after harvesting. The results of the effects of volatile compounds, non-volatile compounds and bacterial cell-free extracts showed that the tested isolates have the effect of inhibiting the growth of pathogenic mycelium. In this study, S. marcescens AP5 and B. cereus AP13 isolates showed more than 70% inhibition of pathogenic mycelium growth. The effect of these two bacteria on the rate of apple brown rot in storage conditions was 84.25 and 77.85%, and the effect of methyl thiophate fungicide was 85.62%. The results of this research show that these two bacterial isolates protect apples against M. laxa through direct and indirect mechanisms and therefore, they can be used as potential biological control agents (BCAs) in preventive treatment to control brown rot disease in apple fruits.
کلیدواژهها [English]
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