Monitoring the susceptibility of different populations of tomato leaf miner, Tuta absoluta to indoxacarb and its combination with azadirachtin

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه پزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 عضو هیت علمی موسسه تحقیقات گیاه پزشکی کشور

3 هیات علمی دانشگاه

4 هیات علمی دانشگاه دانشگاه محقق اردبیلی

چکیده

Tomato leaf miner, Tuta absoluta, is one of the most detrimental biological agents in tomato cultivation, and there have been many reports of its worldwide resistance to various insecticides. This study aimed to evaluate the resistance of six T. absoluta populations collected from four provinces of Iran, including Ardabil, Tehran, Alborz, and Khuzestan to the widely-used insecticide indoxacarb. Leaf-dipping method bioassay was performed against the second-instar larvae. Estimated 72 h-LC50 values had not any overlapping between their 95% confidence intervals in all populations, indicating the presence of significant resistance in six populations compared to the susceptible one. The highest to lowest resistance ratios were obtained for the populations of Ziba Shahr (25.83), Mohammad Shahr (13.08), Parsabad Moghan (8.68), Safiabad (8.52), Ardabil (4.23), and Benoot-e Bala (2.19), respectively. The indoxacarb mixed with azadirachtin at the LC10: LC10 ratio showed an additive effect, while the LC25: LC25 ratio showed a synergistic effect. Also, the larval mortality caused by a mixture of LC25 values of indoxacarb and azadirachtin was significantly higher than mortality due to their separately used LC50 values in the susceptible population. In conclusion, some indoxacarb-resistant levels were documented in field second-instar larvae of T. absoluta. However, adding azadirachtin enhanced insecticidal efficiency of indoxacarb, which may be applicable in the suitable and safe management of this detrimental insect pest.

کلیدواژه‌ها


عنوان مقاله [English]

Tuta absoluta پایش حساسیت جمعیت های مختلف شب پره مینوز گوجه فرنگی به ایندوکساکارب و اختلاط این حشره کش با آزادیراکتین

نویسندگان [English]

  • Mohsen Taleh 1
  • Aziz sheikhigarjan 2
  • Hooshang Rafiee Dastjerdi 3
  • Asgar Ebadollahi 4
  • Sevil Noruzinia 1
1 Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 Research Associate Professor of Iranian Research Institute of Plant Protection(IRIPP)
4 University of Mohaghegh Ardabili
چکیده [English]

شب­پره­ مینوز گوجه ­فرنگی Tuta absoluta  یکی از آفات مهم گوجه ­فرنگی می­ باشد که گزارش­ های بسیاری از مقاومت این آفت به حشره ­کش­ های مختلف از سراسر جهان شده است. این مطالعه به منظور بررسی احتمال مقاومت شش جمعیت­ T. absoluta جمع آوری شده از چهار استان ایران، شامل اردبیل، تهران، البرز و خوزستان به حشره­ کش ایندوکساکارب انجام شد. زیست سنجی روی مرحله لارو سن دو با روش غوطه­ ورسازی برگ در شرایط آزمایشگاهی صورت گرفت. مقادیر LC50 برآورد شده پس از گذشت 72 ساعت در محدوده اطمینان 95 درصد در تمام جمعیت­ های مناطق مختلف با جمعیت حساس هم پوشانی نداشت که نشانگر وجود مقاومت معنی ­دار در این جمعیت­ ها در مقایسه با جمعیت حساس می­ باشد. نرخ مقاومت به ترتیب از بیشترین به کمترین برای جمعیت زیباشهر (83/25)، محمد شهر (08/13)، پارس ­آباد مغان (68/8)، صفی­ آباد (52/8)، اردبیل (23/4) و بنوت بالا (19/2) به دست آمد. اختلاط ایندوکساکارب و آزادیراکتین با نسبت LC10: LC10 تقابل افزایشی و با نسبت LC25: LC25 تقابل سینرژیستی نشان داد. مرگ و میر لاروهای تیمار شده با مخلوط حشره­ کش ­های ایندوکساکارب و آزادیراکتین با نسبت LC25: LC25 به طور معنی‌داری بالاتر از تیمار جداگانه حشرات با LC50 این ترکیبات در جمعیت حساس بود. در نهایت، سطوحی از مقاومت لاروهای سن دو مینوز گوجه‌فرنگی مناطق مختلف ثبت گردید و از طرف دیگر، حشره­ کش آزادیراکتین باعث افزایش سمیت ایندوکساکارب شد که می­ تواند منجر به مدیریت مناسب و ایمن این آفت شود.

کلیدواژه‌ها [English]

  • آزادیراکتین
  • ایندوکساکارب
  • نسبت مقاومت
  • جمعیت حساس
  • شب پره مینوز گوجه فرنگی
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