بررسی اثر بیمارگر Fusarium verticillioides روی فعالیت آنزیم سوپراکسید دیسموتاز و پروتئین‌های برگ دو لاین ذرت با استفاده از تکنیک الکتروفورز دوبُعدی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

2 بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اردبیل (مغان)، سازمان تحقیقات، آموزش و ترویج کشاورزی، مغان، ایران.

3 گروه علوم کشاورزی، دانشگاه پیام نور، تهران، ایران.

چکیده

به­منظور ارزیابی اثر فوزاریوم Fusarium verticillioides بروی وزن تر، شاخص کلروفیل، فعالیت ایزوفرم­های سوپراکسید دیسموتاز روی ژل پلی ­آکریلامید و پروتئین­های برگ به روش الکتروفورز دو بُعدی در لاین­های ذرت، آزمایش به­صورت فاکتوریل در قالب طرح کاملاً تصادفی در پنج تکرار تحت شرایط گلخانه­ای به اجرا در آمد. عامل اول دو لاین ذرت B73 وMO17 و عامل دوم آلودگی به F. verticillioides در دو سطح بدون آلودگی و آلودگی در مرحله گیاهچه­ای بودند. نتایج حاصل نشان داد که اثر بیمارگر F. verticillioides روی وزن تر گیاهچه­های ذرت غیرمعنی­دار بود ولی میزان شاخص کلروفیل به­طور معنی­دار در لاین­های ذرت تیمار شده با عامل بیماری کاهش یافت. تجزیه الکتروفورزی آنزیم سوپراکسید دیسموتاز نشان داد که سه ایزوفرم روی ژل پلی آکریلامید هشت درصد وجود داشت. فعالیت ایزوفرم­های سوپر اکسید دیسموتاز تحت بیماری فوزاریومی افزایش معنی­داری داشت. تجزیه پروتئوم برگ لاین­های ذرت نشان داد که هشت لکه تکرارپذیر معنی­دار تحت آلودگی بیماری فوزاریومی تغییر بیان داشتند. پروتئین­های کاندید جزء پروتئین­های دخیل در سیستم دفاعی و تنظیم بودند. بیشترین اثر پروتیئن­ها در لاین­های ذرت مربوط به پروتئین­های درگیر در سیستم دفاعی از قبیل Isoform 2 of Mitogen-activated protein kinase 12 و Peroxiredoxin-2E, Chloroplastic بودند. براساس نتایج حاصل چنین به­نظر می­رسد که افزایش فعالیت ایزوفرم­های سوپر اکسید دیسموتاز و پروتئین­های دخیل در سیستم دفاعی احتمالاً بتوانند اثر بیماری فوزاریومی را در گیاهچه­های ذرت کاهش دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Impact of Fusarium verticillioides on superoxide dismutase activity and leaf proteins of two corn lines by two-dimensional gel electrophoresis

نویسندگان [English]

  • Afsaneh Rezaei 1
  • Ali Asghari 1
  • Sajjad Moharramnejad 2
  • Vahid Nasrollahzadeh Asl 3
  • Mehri Yusefi 3
1 Department of Genetics and Plant Production, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Crop and Horticultural Science Research Department, Ardabil Agricultural and Natural Resources and Education Center, AREEO, Moghan, Iran.
3 Department of Agricultural Sciences, Payame Noor University, Tehran, Iran.
چکیده [English]

To evaluate the effect of Fusarium verticillioides on fresh weight, chlorophyll index, the activity of superoxide dismutase isoforms, and leaf proteins using two-dimensional gel electrophoresis in two corn lines, a factorial experiment based on a completely randomized design with five replications was performed with two corn lines B73 and MO17, infected with and without F. verticillioides under greenhouse conditions. The results showed that the effect of F. verticillioides on fresh weight was not significant, but it was significant on chlorophyll index in both corn lines. Electrophoretic analyses were carried out using 8% slab polyacrylamide gels. Superoxide dismutase was observed in three isoforms into polyacrylamide gels, and also these isoform activates were increased in plants infected by F. verticillioides. Leaf tissue proteome analysis of two corn lines showed that eight reproducible protein spots had significant alterations under F. verticillioides. These eight differentially expressed proteins were involved in the defense system and regulation processes. Isoform 2 of Mitogen-activated protein kinase 12 and Peroxiredoxin-2E, chloroplastic proteins involved in the defense system had overexpression under F. verticillioides in both corn lines. It seems that increased superoxide dismutase isoforms and proteins involved in the defense system can reduce F. verticillioides damage in the corn seedlings. 

کلیدواژه‌ها [English]

  • Chlorophyll index
  • Conferring host
  • Pathogen
  • Proteome analysis
  • Seedling
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