مقایسه‌ تأثیر فیتوهورمون GR24 و دو گونه قارچی به تنهایی و در ترکیب با هم در افزایش مقاومت دو رقم گوجه‌فرنگی نسبت به بیماری پژمردگی فوزاریومی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران

2 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل. زابل، ایران

3 گروه زیست شناسی، دانشگاه یزد، یزد، ایران.

4 گروه مدیریت منابع خشک و بیابانی، دانشکده منابع طبیعی و کویر شناسی، دانشگاه یزد.

5 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زابل. زابل، ایران .

6 گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان.

چکیده

در این تحقیق تأثیر فیتوهورمون استریگولاکتون و دو گونه قارچ در القاء مقاومت دو رقم گوجه‌فرنگی نسبت به بیماری پژمردگی فوزاریومی در شرایط گلخانه‌ای مورد بررسی قرار گرفت. در این آزمایش از دو رقم گوجه‌فرنگی شامل کاپتین و 4129 و تیمارهای قارچTrichoderma reesei، Rizophagus irregularis و فیتوهورمون GR24 (6-10 ‌میکرومولار) استفاده شد. آزمایش به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار و در سه بازه زمانی بعد از مایه‌زنی عامل بیماری (صفر، 7، 15 و 35 روز) اجرا شد. تأثیر تیمارهای مورد بررسی روی تغییرات چند آنزیم آنتی‌اکسیدانی، فنل، پروتئین کل و همچنین شدت بیماریزایی مورد بررسی قرار گرفت. نتایج حاصل از تجزیه و تحلیل داده‌های به‌دست آمده از چهار بازه زمانی نشان داد که بیشترین مقدار هر سه آنزیم با مقادیر متفاوت برای دو رقم در بازه زمانی 35 روز بعد از مایه‌زنی اتفاق می‌افتد. در مقایسه تیمارهای اعمال شده در این بازه زمانی، در بین تیمارهای ترکیبی و انفرادی به ترتیب کاربرد همزمان سه عامل مورد بررسی در مقایسه با سایر تیمارها، اثر قابل ملاحظه­ای را در افزایش پارامترهای اندازه­گیری شده نشان دادند. با توجه به نتایج این تحقیق، چنین پیشنهاد می­شود که کاربرد هر سه عامل به صورت ترکیبی می‌تواند علاوه بر ایجاد مقاومت به بیماری، به عنوان عامل محدودکننده رشد قارچ‌ بیمارگر در داخل گیاه در نظر گرفته شود.

کلیدواژه‌ها


عنوان مقاله [English]

Comparative effect of gr24 phytohormone and two fungal species alone or in combination in increasing resistance of two tomato cultivars against fusarium wilt disease

نویسندگان [English]

  • seyedreza Mirrahimi bidakhavidi 1
  • Mehdi Pirnia 2
  • Seyed Kazem Sabbagh 3
  • Seyed Ebrahim Seifati 4
  • Mojtaba Keikha Saber 5
  • Abdolhossein Taheri 6
1 Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran
2 Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran.
3 Department of Biology, Yazd University,, Yazd, Iran
4 Department of Air Land and desert Management, Faculty of Natural Sciences and Desert Studies, Yazd University,
5 Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran.
6 Department of Plant Protection, Faculty of Plant Products, Gorgan University of Agricultural Science and Natural Resources.
چکیده [English]

In this study, the effect of two fungal species and also a strigolacton phytohormone was evaluated on inducing resistance in two tomato cultivars infected with fusarium wilt disease. In this greenhouse experiment, two cultivars of tomato namely, Captain and 4129 and Trichoderma reesei, Rhizophagus irregularis and GR24 phytohormone (106µM) were used. For this purpose, a factorial experiment was used in a completely randomized design with three replications in different time periods after inoculation of the pathogen (0, 7, 15, 35 days). The effect of biocontrol treatments on changes of some antioxidant enzymes, phenol, total protein and disease severity was assayed. Data analysis showed that the highest activity of all three enzymes with different values for both cultivars occurred during 35 days after inoculation. Among combined and single treatments, application of a combination of three biocontrol factors (T. reesei, R. irregularis and GR24 phytohormone), respectively showed significant effect on increase of measured parameters compared with other treatments. Based on the results of this study it is suggested that application of all three agents in liquid form can be considered as a limiting factor for the growth of the pathogen on the seed surface besides inducing resistance against disease.

کلیدواژه‌ها [English]

  • Trichoderma
  • Disease severity
  • Biological control
  • Acquired resistance
  • Mycorrhiza
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