کنترل زیستی نماتد ریشه مرکبات و نماتد ریشه گرهی در میزبان‌های لیموترش و کیوی با استفاده از جدایه های Streptomyces

نوع مقاله : مقاله پژوهشی

نویسندگان

1 پژوهشکده‌ مرکبات‌ و ‌میوه‌های ‌نیمه‌گرمسیری، ‌موسسه ‌تحقیقات ‌علوم ‌باغبانی، ‌سازمان ‌تحقیقات، ‌آموزش ‌و‌ ترویج‌کشاورزی، ‌رامسر، ‌ایران.

2 گروه گیاه پزشکی، دانشگده کشاورزی، دانشگاه تبریز، تبریز، ایران.

3 گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه گیلان، رشت. ایران.

چکیده

چکیده
ﺑﻪ­ﻣﻨﻈﻮر ﺑﺮرﺳﻲ تأثیر استرپتومایسس‌ها در کنترل زیستی Tylenchulus semipenetrans در لیموترش (Citrus aurantifolia) و Meloidogyne incognita در کیوی (Actinidia deliciosa)، ﻧﻤﻮﻧﻪﺑﺮداری از ﺧﺎک و رﻳﺸﻪ در ﺑﺎغﻫﺎی ﻣﺮﻛﺒﺎت مازندران، ﮔﻴﻼن و گلستان انجام شد. در شرایط آزمایشگاهی، 9 جدایه استرپتومایسس با کاهش تفریخ تخم و مرگ میر لاروهای نماتد مرکبات و ریشه‌گرهی، از توان آنتاگونیستی خوبی برخوردار بودند. جدایه‌های Streptomyces sp. (D, E, G, H و C) که در شرایط آزمایشگاهی بیشترین توان کنترلی را داشتند، در گلخانه مورد بررسی قرار گرفتند. از بین جدایه‌های مورد بررسی، جدایه­ های Streptomyces sp. ( Dو G) در شرایط گلخانه ﺑﻪ ﺗﺮﺗﯿﺐ ﺑﺎ کاهش تعداد نماتد ماده ﺑﻪ ﻣﯿﺰان 81/86 و 65/77 درصد در ﻣﻘﺎﯾﺴﻪ ﺑﺎ نماتدکش ﻓﻨـﺎﻣﯿﻔﻮس 47/64 درصد، بیشترین تأثیر را در کنترل نماتد مرکبات نشان دادند. همچنین این دو جدایه ﺑﻪ ﺗﺮﺗﯿﺐ با کاهش تعداد گال ﺑﻪ ﻣﯿﺰان 78/67 و 15/71 درصد نسبت به نماتدکش فنامیفوس 93/50 درصد و کاهش تعداد کیسه تخم ﺑﻪ میزان 54/86 درصد در مقایسه با نماتدکش فنامیفوس 90/68 درصد، در کاهش جمعیت نماتد ریشه­ گرهی در شرایط گلخانه از عملکرد خوبی برخوردار بودند.

کلیدواژه‌ها


عنوان مقاله [English]

Biological control of citrus root nematode and root knot nematode in acid lime and kiwifruit hosts using Streptomyces isolates

نویسندگان [English]

  • Morteza Golmohammadi 1
  • Sina Noorizadeh 2
  • Seyedeh Najmeh Banihashemian 3
1 Citrus and Subtropical Fruits Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization, Ramsar, Iran.
2 Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
3 Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht, Iran.
چکیده [English]

Abstract
To evaluate the effects of Streptomyces bacteria on biocontrol of Tylenchulus semipenetrans in Citrus aurantifolia and Meloidogyne incognita in Actinidia deliciosa, samples were collected from the rhizosphere of citrus and kiwifruit trees in Mazandaran, Guilan, and Golestan provinces. In vitro, 9 Streptomyces isolates showed good antagonistic potential and reduced the rate of egg hatching and larval mortality of citrus nematode and root-knot nematode. Isolates of Streptomyces sp. (C, D, E, G, and H) which had high control ability in vitro were examined in the greenhouse. Among the isolates studied, isolates D and G in greenhouse conditions showed the greatest effect on the control of citrus nematodes by reducing the number of female nematodes by 86.81% and 77.65% compared with the fenamiphos 64.47% Also, these two isolates decreased galls by 67.78% and 71.15% compared with the fenamiphos 50.93% and reduced the number of egg masses by 86.54% ccompared with the phenamiphus 68.90%, had good performance in root-knot nematode control.

کلیدواژه‌ها [English]

  • Keywords: Antagonist
  • Greenhouse Conditions
  • Molecular Identification
  • Meloidogyne incognita
  • Tylenchulus semipenetrans
 
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