بررسی پتانسیل آنتاگونیستی باکتری‌های اپی‌فیت همراه با درختان میوه دانه‌دار در استان آذربایجان غربی بر علیه Erwinia amylovora و ارزیابی تنوع ژنتیکی آنها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بخش گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران.

2 بخش تحقیقات بیماریهای گیاهان، موسسه تحقیقات گیاه پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران.

چکیده

چکیده
در شاخ و برگ­های درختان میوه دانه ­دار، باکتری­های اپی­فیت زیادی وجود دارند که خاصیت آنتاگونیستی آنها علیه عامل بیماری آتشک (Erwinia amylovora) اثبات شده است. به منظور شناسایی، بررسی پتانسیل آنتاگونیستی و تنوع ژنتیکی باکتری­های اپی­فیت آنتاگونیستی همراه با درختان میوه­ دانه دار در استان آذربایجان غربی، از باغات سیب، گلابی و به در سه نوبت بهار، تابستان و پاییز سال 1393 نمونه­برداری به عمل آمد. پس از جداسازی باکتری­های اپی­فیت و انجام آزمون آنتاگونیستی، شناسایی جدایه­ها صورت گرفت. جدایه ­ها در دو گروه باکتری­های گرم منفی (Pantoea agglomerans، Pseudomonas fluorescens) و گرم مثبت دسته ­بندی شدند. تنوع ژنوتیپی جدایه­ های Pantoea  و Pseudomonas از مناطق مختلف با استفاده از روش Rep-PCR مورد بررسی قرار گرفت. جدایه ­ها با استفاده از برنامه­  نرم ­افزاری NTYsys به روش UPGMA و ضریب تشابه جاکارد مورد بررسی قرار گرفتند. تجزیه و تحلیل اطلاعات بدست آمده بر اساس نشانگر BOX1 نشان دهنده وجود 10 گروه با حداقل شباهت 75 درصد در میان جدایه ­های Pseudomonas و وجود 9 گروه در میان جدایه­ های Pantoea  با حداقل شباهت 70 درصد بود. نتایج تنوع ژنتیکی بالایی را در میان سویه­ های آنتاگونیست اپی­فیت درختان میوه دانه­ دار در استان آذربایجان غربی نشان داد.
 

کلیدواژه‌ها


عنوان مقاله [English]

Antagonistic potential of epiphytic bacteria associated with pome fruit trees in West Azarbaijan province against Erwinia amylovora and evaluation of their genetic diversity

نویسندگان [English]

  • Habib Nojavan Chichaklou Mansour 1
  • Abbasali Ravanlou 2
  • Saeed T arighi 1
1 1Department f Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Department of Plant Diseases Research, Iranian Research Institute of Plant Protection, Agricultural Research, education and Extension Organization (AREEO), Tehran, Iran.
چکیده [English]

Abstract
There are many epiphytic bacteria live on surface of pome fruit tree foliage, and have been proven to show antagonistic activity against Erwinia amylovora, the causal agent of fire blight disease. In order to characterize and assess antagonistic potential and genetic diversity of epiphytic bacteria associated with pome fruit trees in orchards of West Azarbaijan province samplings were carried out from foliage of apple, pear, and quince trees during spring, summer and autumn in 2014. Epiphytic bacteria were isolated and their antagonistic potential against E. amylovora were evaluated and then bacterial isolates were identified. The isolated bacteria were categorized into three group including Pantoea agglomerans, Pseudomonas fluorescens and other unknown Gram-positive bacteria. Genetic diversity among the Pseudomonas and Pantoea isolates were studied using Rep-PCR technique. Resulted data were analyzed in NTSYSpc v2.02e software and compared using UPGMA and Jaccard similarity coefficient methods. Also, the analyses of the BoxA1R primer’s band patterns separated ten groups with at least 75% similarity among the pseudomonad isolates. Similarly, nine groups with 70% similarity within the Pantoea isolates were discovered. These results showed a high genetic diversity among the epiphytic strains of Pseudomonas and Pantoea in west Azarbaijan pome fruit orchards.
 

کلیدواژه‌ها [English]

  • Keywords: Epiphyte
  • Fire blight
  • Pome fruit
  • Pantoea agglomerans
  • Pseudomonas fluorescens
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