ارزیابی و شناسایی لاین ها و هیبریدهای مقاوم به بیماری پوسیدگی فوزاریومی بلال ذرت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 بخش تحقیقات زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی گلستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، گرگان

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه همدان.

3 گروه بیوتکنولوژی و اصلاح نباتات، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان.

4 بخش تحقیقات گیاهپزشکی مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان.

چکیده

چکیده
پوسیدگی بلال، ناشی از قارچ Fusarium verticillioides، یکی از مهم­ترین بیماری­ های ذرت است که سبب آلودگی دانه به مایکوتوکسین فومونیزین شده  که برای سلامتی انسان و حیوان مصرف­کننده مضر می­ باشد. به ­منظور تعیین قابلیت ترکیب ­پذیری عمومی و خصوصی ده لاین این برد ذرت در واکنش به بیماری فوزاریومی بلال ذرت، از یک طرح دای­آلل 10×10 در سال 1393 استفاده و 90 ترکیب حاصل از تلاقی دوطرفه 10 لاین مورد نظر در سال 1394 در ایستگاه تحقیقات کشاورزی عراقی محله گرگان مورد ارزیابی قرار گرفتند. صفت شدت بیماری بر اساس مقیاس 6-1 بررسی شد. نتایج تجزیه دای­آلل نشان داد که اثرات افزایشی و غیرافزایشی همزمان در کنترل این صفت مؤثر هستند، اما نقش اثرات غالبیت بارزتر می ­باشد. از بین لاین ­های مورد بررسی، دو لاین C5 و C53 بالاترین ترکیب ­پذیری منفی و معنی ­دار را در واکنش به بیماری نشان دادند. بالاترین میزان هتروزیس نیز در دو تلاقی C7×B73 و C7×C5 مشاهده گردید.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation and identification of resistant lines and hybrids to fusarium ear rot disease of maize

نویسندگان [English]

  • Seyed Afshin Mosavat 1
  • Hojatollah Mazahery-Laghab 2
  • Hassan Soltanloo 3
  • Mohammad Ali Aghajani Nasab-Afroozi 4
1 Agronomy & Horticulture Sciences Research Department, Golestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Gorgan-Iran
2 Agronomy and Plant Breeding Department, Agriculture Faculty, Bu-Ali Sina University
3 Plant Breeding and Biotechnology Group, Plant Production College, Gorgan University of Agricultural Sciences and Natural Resources.
4 Golestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Gorgan-Iran.
چکیده [English]

Abstract
Fusarium ear rot disease of maize  caused by Fusarium verticillioides is one of the most important maize diseases that cause grain contamination with fumonisin mycotoxin, which is harmful for  humans and animals. In order to determine the general and specific combining ability of 10 inbred lines of maize in response of fusarium ear rot disease, a 10×10 Diallel Cross design was used in 2014 and 90 combinations resulted from a two-way crosses of 10 interested inbred lines were evaluated  at Golestan Agricultural and Natural Resources Research and EducationCenter, Agricultural Research Station of Araghi-Mohaleh in 2015. Trait of the severity of the disease was assessed on a scale of 1-6. Diallel analysis results based on method one and the mixed model B Griffing showed that additive and non-additive effects are effective in controlling of this trait,but the role of dominance is more pronounced.From the investigated lines two C5 and C53 inbred lines showed the highest significant negative combining ability in response to the disease. The highest heterosis amount was observed in two crossings of C7 × B73 and C7 × C5.

کلیدواژه‌ها [English]

  • Keywords: Combining ability
  • Mycotoxin fumonizine
  • Heritability
  • Severity of the disease

مراجع

Azimi, S, 2012. Investigation on Fusarium ear rot of corn in Khuzestan. Cerreal Science, 1(1): 75–83(In Persian with English abstract).
Choukan, R, 2008. Estimation of heterosis and combining ability in resistance of Fusarium rot of corn cob. Seed and Plant Journal 23 (4) 603–613 (In Persian with English abstract).
Choukan, R, 2013. Corn and its characteristics. Ministry of Jihad Agriculture, Agricultural Research, Education and Extension Organization, Seed and Plant Improvement Research Institute, Agricultural Education Publishing. 466 p. (In perisan).
De la Campa  R, Hooker  DC, Miller  JD, Schaafsma  AW and Hammond  BG, 2005. Modeling effects of environment, insect damage, and Bt genotypes on fumonisin accumulation in maize in Argentina and the Philippines. Mycopathologia (159): 539–552.
Fandohan  P, Hell  K, Marasas WFO,Wingfield MJ, 2003. Infection ofmaize by Fusarium species and contamination with fumonisis in Africa. African Journal of Biotechnology (12): 570–579.
Farrar  JJ, Davis  RM, 1991. Relationship among ear morphology, western plower thrips, and fusarium ear rot of corn. Phytopathology (81): 661–666.
Gendloff  E, Rossman  WL, Casale  TG,  Jeffers  D, Vasal  SK, et al., 1994. Evaluation of tropical inbred lines for resistance to Fusarium moniliforme ear rot. Maize Genetics Cooperation Newsletter 68: 58.
Gendloff  EH, Rossman  EC, Casale  WL, Isleib  TG, Hart  LP, 1986. Components of resistance to fusarium ear rot in field corn. Phytopathology (76): 684–688.
Gevers  HO, Lake  JK, McNab  NJ, 1990. An analysis of ear rot and leaf blight resistance in department maize breeding material. Proceedings of the S. A. Maize Breeding Symposium. pp. 41–46.
Griffing  B, 1956. Concept of general and specific combining ability in relation to diallel crossing system. Australian Journal of Biological Science (9): 463–493.
Hayman  B I, 1958. The theory and analysis of diallel crosses II. Genetics (42): 63–85.
Kleinschmidt  CE, Clements  MJ, Maragos  CM, Pataky  JK, White  DG, 2005. Evaluation of food-grade dent corn hybrids for severity of Fusarium ear rot and fumonisi accumulation in grain. Plant Disease (89): 291–297.
Koehler  B, 1959. Corn earrot in Illinois.Univ.III. Agri. Exp. Sta. Bull. No. 639. Champanig 3 Illinois, USA.
Lanubile  A, Ferrarini  A, Maschietto  V, Delledonne  M, Marocco A, et al., 2014. Functional genomic analysis of constitutive and inducible defense responses to Fusarium verticillioides infection in maize genotypes with contrasting ear rot resistance. BMC Genomics (15): 1–16.
Lanubile  A, Pasini  L, Marocco A, 2010. Differential gene expression in kernels and silks of maize lines with contrasting levels of ear rot resistance after Fusarium verticillioides infection. Journal of Plant Physiology (167): 1398–1406.
Lunsford  JN, Futrell  MC, Scott  GE, 1974. Maternal influence on response of corn to Fusarium moniliforme. Phytopathology (65): 223–225.
McGee  DC, 1988. Maize Diseases: A Reference Source for Seed Technologist. The American Phytopathological Society, St. Paul, Minnesota, USA.
Mosavat, A. 2018. Genetic analysis & molecular evaluation of resistance to fusarium ear rot disease (Fusarium verticilioides) in Some Corn Hybrids & Lines (Zea mays L.). PhD thesis, of BU-Ali Sina University. (In Persian with English abstract).
Munkvold  GP, 2003. Cultural and genetic approaches to managing mycotoxins in maize. Annu Rev Phytopathol (41): 99–116.
Murillo-Williams A, Munkvold P, 2008. Systemic infection by fusarium verticillioides in maize plants grown under three temperature regimes. Plant Disease, (92): 1695–1700.
Pascale  M, Visconti  A, Prończuk  M, Wiśniewska  H, Chelkowski  J, 1997. Accumulation of fumonisins in maize hybrids inoculated under field conditions with Fusarium moniliforme Sheldon. Journal of the Science of Food and Agriculture (74): 1–6.
Perez-Brito D, Jeffers D, Gonzales-de-Leon D, Khairallah M, Cortes-Cruz M, et al., 2001. QTL mapping of Fusarium moniliforme ear rot resistance in highland maize, Mexico. Agrociencia 201(35): 181–96.
Pesaraklu S, Soltanloo H, Ramezanpour SS, Mosavat A, Tavakol T, 2020. Combining ability and assessment of resistance crosses derived of multi-line of corn maize to fusarium ear rot disease. Journal of Crop Breeding 12 (34): 130–137 (In Persian with English abstract).
Robertson Leilani A, Kleinschmidt Craig E, White Don G, Payne Gary A, Maragos Chris M, et al., 2006. Heritabilities and correlations of Fusarium ear rot resistance and fumonisin contamination resistance in two maize populations. Crop Science 46(1): 353–361.
Shelby  RA, 1994. Differential fumonisin production in maize hybrids. Plant Disease 78: 582–584.
Venturini D, Assante G, Vercesi A, 2011. Fusarium verticillioides contamination patterns in northern Italian maize during the growing season. Phytopathology Mediterr (50): 110–120.
Williams  LD, Glenn  AE, Zimeri  AM, Bacon  CW, Smith  MA, et al., 2007. Fumonisin disruption of ceramide biosynthesis in maize roots and the effects on plant development and Fusarium verticillioide induced seedling disease. Agriculture Food Chemistry (55): 2937–2946.
Yi Hung, H,  Holland JB, 2012. Diallel analysis of resistance to fusarium ear rot and fumonisin contamination in maize. Crop Science 52: 2173–2181.
Zamani, M, Choukan R, 2005. Investigation of combinability and genetic variance at the intersection of tester and line to determine the sources of resistance to Fusarium rot of corn cob.Journal of Research and Construction 66: 97–103 (In Persian with English abstract).
Zamani, M, Choukan R, 2013. The role of parents in the response of maize cultivars to Fusarium caries disease. Seed and Plant Breeding Journal 29 (1): 13–24 (In Persian with English abstract).

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  • تاریخ دریافت: 01 فروردین 1400
  • تاریخ بازنگری: 03 اسفند 1399
  • تاریخ پذیرش: 03 اسفند 1399

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