تنوع ژنتیکی جمعیت های گونه Sphaerulina frondicola عامل بیماری لکه برگی صنوبر در استان آذربایجان شرقی با استفاده از نشانگرهای مولکولی RAPD و ISSR

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، بیماری شناسی گیاهی، استاد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز

2 استاد، بیماری شناسی گیاهی، استاد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز

3 پژوهشگر پسا دکتری بیماری شناسی گیاهی، استاد، گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه تبریز

چکیده

چکیده
بیماری­ لکه برگی صنوبر، با عاملSphaerulinafrondicola (syn: Septoria populi) ازجمله بیماری‌های مهم و جدی درختان صنوبر می­ باشد که خسارت زیادی به این میزبان وارد می­نماید. در این بررسی تنوع ژنتیکی 138جدایهS. frondicola  بدست آماده از درختان صنوبر در مناطق مختلف استان های آذربایجان شرقی و غربی با استفاده از نشانگرهای مولکولی RAPD وISSR  ارزیابی شد. نتایج این بررسی نشان داد که تنوع ژنتیکی قابل توجهی در بین جمیت ­های مورد مطالعه وجود دارد. میانگین تنوع ژنتیکی برآورد شده برای کل جمعیت­ ها با استفاده از داده ­های همه آغازگر­ها 199/0 در سطح احتمال 001/0 برآورد گردید. کمترین تمایز ژنتیکی بین جمعیت‌های هوراند و کلیبر (352/0) با بیشترین جریان ژنی (956/54) و بیشترین تمایز ژنتیکی بین جمعیت‌های خوی و بستان آباد (516/0) با کمترین جریان ژنی (468/0) مشاهده شد که با فاصله جغرافیایی همخوانی دارد. بر اساس فاصله ژنتیکی Nei، جمعیت بستان آباد بیشترین فاصله را با شش جمعیت دیگر نشان داد. آنالیز هاپلوتیپ­ ها، جدایه ­های به دست آمده از یک درخت و یا حتی از یک لکه را درگروه­ های متفاوتی قرار داد که نشان دهنده وجود تنوع ژنتیکی بالا در یک مقیاس کوچک می­باشد. به طور کلی وجود هاپلوتیپ ­های مختلف و توزیع تنوع ژنی در یک مقیاس کوچک، فرضیه حضور آسکوسپور جنسی هوازاد به عنوان مایه تلقیح اولیه در چرخه بیماری را نشان می­ دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Genetic diversity among Sphaerulina frondicola populations, the causal agent of poplar leaf spot disease in East Azarbaijan Province using RAPD and ISSR molecular markers

نویسندگان [English]

  • Mina Mirzaei 1
  • Asadollah Babai-Ahari 2
  • Abolfazl Narmani 3
  • Mahdi Arzanlou 2
1 MSc, Faculty of Agriculture, Tabriz University
2 Professor, Faculty of Agriculture, Tabriz University
3 Postdoctoral Researcher, Faculty of Agriculture, Tabriz University
چکیده [English]

Abstract
Poplar leaf spot disease, caused by Sphaerulinafrondicola (syn: Septoria populi), is one of the important and serious diseases of populus trees, which cause significant loss on this host. Considering the importance of this disease, in the present study, the genetic diversity of 138 isolates S. frondicola isolates obtained from populus trees different regions of East and West Azerbaijan provinces was analysed using RAPD and ISSR molecular markers. The results indicated considerable genetic variations amongst the studied populations. The mean genetic diversity for all of the populations using data from all primers was 0.199 at the probability level of 0.001. The lowest genetic differentiation was observed between Horand and Kaleyber populations (0.352) with the highest gene flow (54.956) and the highest genetic differentiation was observed between Khoy and Bostanabad populations (0.516) with the lowest gene flow (0.468) which is consistent with geographical distance.Based on Nei's genetic distance, the Bostanabad population showed the highest genetic distance from the other six populations. Haplotype analysis clustered the isolates from a single tree or even single spot in different groups, suggesting the existence of high genetic diversity in a small scale. Overall, the existence of different haplotypes and distribution of genetic diversity in a small scale observed in this study, suggests occurrence of regular sexual cycle in S. frondicola populations and highlights the role of airborne ascospores as primary inoculum source in disease cycle. 

کلیدواژه‌ها [English]

  • Keywords: Populus
  • leaf spot
  • East Azerbaijan Province
  • Septoria
  • genetic diversity
  • RAPD-PCR
  • ISSR-PCR
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