بررسی کارایی حشره‌کشی نانوذرات اکسید روی سنتز شده با استفاده از عصاره جلبک قهوه‌ای Polycladia myrica علیه سوسک چهار نقطه‌ای حبوباتCallosobruchus maculatus (Col.: Chrysomelidae: Bruchinae)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه خلیج فارس، بوشهر، ایران

2 گروه شیمی، دانشگاه خلیج فارس، بوشهر، ایران

3 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران

4 گروه جانورشناسی، پردیس Pt. L.M.S.، دانشگاه سری دو سومان اوتاراکند، ریشیکش، اوتاراکند، هند

چکیده

خطرات زیست‌محیطی و سلامت انسان مرتبط با حشره‌کش‌های شیمیایی، بررسی­ های گسترده‌ای را برای یافتن روش‌های جایگزین برای محافظت از محصولات انباری برانگیخته است. اخیراً، نانوذرات به عنوان جایگزین‌های امیدوارکننده‌ای برای محصولات شیمیایی مصنوعی در نظر گرفته شده‌اند. در این مطالعه، نانوذرات  روی (ZnO NPs) با استفاده از عصاره جلبک Polycladia myrica سنتز شدند و با استفاده از پراش اشعه ایکس (XRD)، طیف ­سنجی مادون قرمز (FTIR)  و میکروسکوپ الکترونی روبشی نشر میدانی (FE-SEM) شناسایی شدند. سه نوع مختلف از نانوذرات روی، ZnO-A، ZnO-B  وZnO-C، با استفاده از عصاره جلبک و با دو روش مختلف سنتز شدند. فعالیت حشره‌کشی آنها علیه سوسک چهار نقطه ­ای حبوبات،Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae)  روی لوبیا چشم بلبلی انبارشده ارزیابی و با نانوذرات روی سنتز شده به صورت شیمیاییZnO-D  مقایسه شد. نانوذرات ZnO-A،ZnO-B  و ZnO-C بیوسنتز شده، فعالیت بالاتری را علیه C. maculatus  در مقایسه با نانوذرات ZnO-D نشان دادند. نانوذرات متخلخل ZnO-A با کوچکترین اندازه ذرات (3/24 نانومتر) به عنوان سمی‌ترین نانوذرات تعیین شدند که پس از پنج روز قرار گرفتن در معرض آنها، بالاترین میزان مرگ و میر حشرات بالغ C. maculatus را ایجاد کردند، در حالی که نانوذرات ZnO-D کمترین تأثیر را در کنترل C. maculatus داشتند. کاهش قابل توجه تخم‌ریزی (1/35 تا 9/44 درصد) و کاهش نتاج (7/35 تا 6/45 درصد) نیز برای حشراتی که در معرض لوبیا چشم بلبلی تیمار شده با نانوذرات ZnO-A با غلظت 500 تا 2000 میلی‌گرم در کیلوگرم قرار داشتند، مشاهده شد. میانگین کاهش وزن دانه‌های لوبیا چشم بلبلی تیمار شده با نانوذرات ZnO-A، ZnO-B  و ZnO-C در غلظت 2000 میلی‌گرم در کیلوگرم به ترتیب 8/23، 1/28 و 5/33 درصد بود که اختلاف معنی‌داری نسبت به شاهد (2/52 درصد) داشتند. نتایج این بررسی نشان می‌دهد که نانوذرات روی بیوسنتز شده می‌توانند به عنوان یک جایگزین مناسب برای کنترل C. maculatus در لوبیا چشم بلبلی انبار شده در نظر گرفته شوند.

کلیدواژه‌ها

عنوان مقاله [English]

Insecticidal efficacy of synthesized ZnO nanoparticles using brown algae Polycladia myrica extract against Callosobruchus maculatus

نویسندگان [English]

  • Fariba Sohrabi 1
  • Zahra Solati 2
  • Fatemeh Bagheri 2
  • Mohammad Ali Mirhosseini 1
  • Masumeh Ziaee 3
  • Ahmad Pervez 4
1 1 Department of Plant Protection, Faculty of Agriculture, Persian Gulf University, Bushehr, Iran
2 Chemistry Department, Persian Gulf University, Bushehr, Iran
3 Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 Department of Zoology, Pt. L.M.S. Campus, Sri Dev Suman Uttarakhand University, Rishikesh, Uttarakhand, India
چکیده [English]

The environmental and human health risks of chemical insecticides have prompted extensive searches for alternative methods to protect stored products. Recently, nanoparticles have been considered as promising alternatives to synthetic chemical products. In this study, ZnO nanoparticles (NPs) were synthesized using Polycladia myrica algae extract, and characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Field emission scanning electron microscopy (FE-SEM). Three types of ZnO NPs, ZnO-A, ZnO-B, and ZnO-C, were synthesized using algae extract by two different methods. Their insecticidal activity was evaluated and compared with the chemically synthesized ZnO-D NPs against the cowpea weevil, Callosobruchus maculatus on stored cowpeas. Biosynthesized ZnO-A, ZnO-B, and ZnO-C NPs exhibited higher activity against C. maculatus when compared with ZnO-D NPs. Porous ZnO-A NPs with the smallest particle size (24.3 nm) were determined to be the most toxic nanoparticles, causing the highest mortality rate of C. maculatus adults after five days of exposure, while ZnO-D NPs were the least effective for C. maculatus control. A significant oviposition inhibition (35.1 to 44.9%) and progeny reduction (35.7 to 45.6%) was also observed for insects exposed to ZnO-A NPs-treated cowpeas at 500 to 2000 mg kg-1. The average weight loss of cowpea seeds treated with ZnO-A, ZnO-B, and ZnO-C NPs at 2000 mg kg-1 was 23.8, 28.1, and 33.5%, respectively, with significant difference compared to untreated control (52.2%). Our results suggest that biosynthesized ZnO NPs can be considered as a viable alternative for controlling C. maculatus in stored cowpeas.

کلیدواژه‌ها [English]

  • Cowpea weevil
  • Polycladia myrica
  • Insect pest control
  • Nanotechnology

مراجع

Abbott WS, 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology 18: 265–267.
Alprol AE, Mansour AT, El-Beltagi HS, Ashour M, 2023. Algal extracts for green synthesis of zinc oxide nanoparticles: promising approach for algae bioremediation. Materials 16(7): 2819.
Arumugam G, Velayutham V, Shanmugavel S, Sundaram J, 2016. Efficacy of nanostructured silica as a stored pulse protector against the infestation of bruchid beetle, Callosobruchus maculatus (Coleoptera: Bruchidae). Applied Nanoscience 6: 445–450.
Attia MA, Wahba TF, Shaarawy N, Moustafa FI, Guedes RNC, et al., 2020. Stored grain pest prevalence and insecticide resistance in Egyptian populations of the red flour beetle Tribolium castaneum (Herbst) and the rice weevil Sitophilus oryzae (L.). Journal of Stored Products Research 87: 101611.
Azimpanah R, Solati Z, Hashemi M, 2022. Synthesis of ZnO nanoparticles with antibacterial properties using Terminalia catappa leaf extract. Chemical Engineering & Technology 45(4): 658–666.
Azizi S, Ahmad MB, Namvar F, Mohamad R, 2014. Green biosynthesis and characterization of zinc oxide nanoparticles using brown marine macroalga Sargassum muticum aqueous extract. Materials Letters 116: 275–277.
Badawy AA, Abdelfattah NA, Salem SS, Awad MF, Fouda A, 2021. Efficacy assessment of biosynthesized copper oxide nanoparticles (cuo-nps) on stored grain insects and their impacts on morphological and physiological traits of wheat (Triticum aestivum L.). Plant Biology 10(3): 233.
Badii BK, Adarkwah C, Obeng-Ofori D, Ulrichs C, 2014. Efficacy of diatomaceous earth formulations against Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) in Kersting’s groundnut (Macrotyloma geocarpum Harms): influence of dosage rate and relative humidity. Journal of Pest Science 87: 285–294.
Bhattacharya P, Chatterjee K, Swarnakar S, Banerjee S, 2020. Green synthesis of zinc oxide nanoparticles via algal route and its action on cancerous cells and pathogenic microbes. Advanced Nano Research 3(1): 15–27.
Dadolahi-Sohrab A, Garavand-Karimi M, Riahi H, Pashazanoosi H, 2012. Seasonal variations in biomass and species composition of seaweeds along the northern coasts of Persian Gulf (Bushehr Province). Journal of Earth System Science 121(1): 241–250.
Das S, Yadav A, Debnath N, 2019. Entomotoxic efficacy of aluminium oxide, titanium dioxide and zinc oxide nanoparticles against Sitophilus oryzae (L.): A comparative analysis. Journal of Stored Products Research 83: 92–96.
Deka B, Babu A, Baruah C, Barthakur M, 2021. Nanopesticides: A systematic review of their prospects with special reference to tea pest management. Frontiers in Nutrition 8: 686131.
Deshpande VK, Makanur B, Deshpande SK, Adiger S, Salimath PM, 2011. Quantitative and qualitative losses caused by Callosobruchus maculatus in cowpea during seed storage. Plant Archives 11(2): 723–731.
Diagne A, Diop BN, Ndiaye PM, Andreazza C, Sembene M, 2019. Efficacy of silica nanoparticles on groundnut bruchid, Caryedon serratus (Olivier) (Coleoptera, Bruchidae). African Crop Science Journal 27(2): 229–235.
Elrefaey AAK, El-Gamal AD, Hamed SM, El-belely EF, 2022. Algae-mediated biosynthesis of zinc oxide nanoparticles from Cystoseira crinite (Fucales; Sargassaceae) and it's antimicrobial and antioxidant activities. Egyptian Journal of Chemistry 65(4): 231–240.
Elsharkawy EE, 2020. Nanotechnology applications of pesticide Journal of Nanomedicine 3(1): 1029.
Fouad EA, Abotaleb AO, 2021. Sublethal effects of two insecticides, deltamethrin, thiamethoxam and the botanical insecticide (Foeniculum vulgare Mill.) on Callosobruchus maculates (Fabr.) (Coleoptera: Bruchidae). Egyptian Academic Journal of Biological Sciences 14(1): 255–269.
Gbaye OA, Millard JC, Holloway GJ, 2011. Legume type and temperature effects on the toxicity of insecticide to the genus Callosobruchus (Coleoptera: Bruchidae). Journal of Stored Products Research 47(1): 8–12.
Gbaye OA, Oyeniyi EA Ojo OB, 2016. Resistance of Callosobruchus maculatus (Fabricius) (Coleoptera: Bruchidae) populations in Nigeria to dichlorvos. Jordan Journal of Biological Sciences 147(3384): 1–6.
Gheda S, Naby MA, Mohamed T, Pereira L, Khamis A, 2021. Antidiabetic and antioxidant activity of phlorotannins extracted from the brown seaweed Cystoseira compressa in streptozotocin-induced diabetic rats. Environmental Science & Pollution Research 28(18): 22886–22901.
Guiry MD, Guiry GM, 2021. AlgaeBase. World‑wide electronic publication, National University of Ireland, Galway.
Harris KL, Lindblad CJ, 1978. Postharvest grain loss assessment methods. A manual of methods for the evaluation of post-harvest losses. American Association of Cereal Chemistry 8(2): 75–79.
Hamza AM, 2012. Efficacy and safety of non-traditional methods as alternatives for control of Sitophilus oryzae (L.) (Coleoptera: Curculionidae) in rice grains. Egyptian Journal of Biological Pest Control 22(2): 103–108.
Hoet PH, Brüske-Hohlfeld I, Salata OV, 2004. Nanoparticles–known and unknown health risks. Journal of nanobiotechnology 2(1): 12.
Husairi FS, Ali SM, Azlinda A, Rusop M, Abdullah S, 2013. Special effect of urea as a stabilizer in thermal immersion method to synthesis porous zinc oxide nanostructures. Journal of Nanomaterials 2013(1): 163527.
Ibrahim S, Elbehery H, Samy A, 2022. Insecticidal activity of ZnO NPs synthesized by green method using pomegranate peels extract on stored product insects. Egyptian Journal of Chemistry 65(4): 135–145.
Ismail T, Salama MA, El-Ebiary M, 2021. Entomotoxic effects of synthesized aluminum oxide nanoparticles against Sitophilus oryzae and their toxicological effects on albino rats. Toxicology & Industrial Health 37(10): 594–602.
Jasrotia P, Nagpal M, Mishra CN, Sharma AK, Kumar S, et al., 2022. Nanomaterials for postharvest management of insect pests: Current state and future perspectives. Frontiers in Nanotechnology 3: 811056.
Kabir BGJ, Wulgo MA, 2014. Efficacy of four diatomaceous earth formulations against Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) on cowpea. In: Proceedings of the 11th International Working Conference on Stored Product Protection, 24-28 November, Chiang Mai, Thailand. Pp. 798-806.
Katoch V, Singh J, Sharma NR, Singh RP, 2024. Synthesis and characterization of mesoporous zinc oxide nanoparticles. Inorganic & Nano-Metal Chemistry 54(1): 58–66.
Khan A, 2021. Biopesticides: Alternatives for management of Callosobruchus maculatus. Journal of Biopesticides 14(1): 59–78.
Khanna P, Kaur A, Goyal D, 2019. Algae-based metallic nanoparticles: Synthesis, characterization and applications. Journal of Microbiological Methods 163: 105656.
Kljajic P, Andric G, Adamovic M, Bodroza-Solarov M, Markovic M, et al., 2010. Laboratory assessment of insecticidal effectiveness of natural zeolite and diatomaceous earth formulations against three stored-product beetle pests. Journal of Stored Products Research 46(1): 1–6.
Lakshmi SJ, Roopa Bai RS, Sharanagouda HCTR, Ramachandra CT, Nadagouda S, 2020. Effect of zinc oxide nanoparticles on pulse beetle (Callosobruchus maculatus) (Col.: Chrysomelidae) in greengram. Journal of Entomological Zoology Studies 8: 297–300.
Lazarevic J, Radojkovic A, Kostic I, Krnjajic S, Mitrovic J, et al., 2018. Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). Journal of Stored Products Research 77: 45–54.
Lewinski N, Colvin V, Drezek R, 2008. Cytotoxicity of nanoparticles. Small 18: 4(1): 26–49.
Lodeiro P, Barriada JL, Herrero R, Sastre de Vicente ME, 2006. The marine macroalga Cystoseira baccata as biosorbent for cadmium (II) and lead (II) removal: Kinetic and equilibrium studies. Environmental Pollution 142(2): 264–273.
Lu J, Sehgal B, Subramanyam B, 2017. Insecticidal potential of a synthetic zeolite against the cowpea weevil, Callosobruchus maculatus (Fabricius) (Coleoptera: Bruchidae). Journal of Stored Products Research 72: 28–34.
Magano DA, Carvalho IR, Doberstein AP, Louro MV, Bubans V, et al., 2021.  Efficiency and persistence of insecticides with different action mechanisms applied on wheat stored pest Sitophilus zeamais. Australian Journal of Crop Science 15(5): 618–621.
Mehrjo F, Hashemi M, Solati Z, Hashemnia S, 2021. Biosynthesis of ZnO nanosheets decorated with pd nanoparticles and their application for electrochemical investigation of ethanol. Electrocatalysis 12: 272–282.
Murdock LL, Seck D, Ntoukam G, Kitch L, Shade RE, 2003. Preservation of cowpea grain in sub-Saharan Africa-bean/cowpea CRSP contributions. Field Crop Research 82(2–3): 169–178.
Noel S, Hermann J, Itina T, 2007. Investigation of nanoparticle generation during femtosecond laser ablation of metals. Applied Surface Science 253(15): 6310–6315.
Ostiguy C, Lapointe G, Trottier M, Ménard L, Cloutier Y, et al., 2006. Health effects of nanoparticles. IRSST Scientific Report No. R-469, Montreal, Canada.
Pandit C, Roy A, Ghotekar S, Khusro A, Islam MN, et al., 2022. Biological agents for synthesis of nanoparticles and their applications. Journal of King Saud University Science 34(3): 101869.
Patra P, Mitra S, Debnath N, Goswami A, 2012. Biochemical-, biophysical-, and microarray-based antifungal evaluation of the buffer-mediated synthesized nano zinc oxide: an in vivo and in vitro toxicity study. Langmuir 28(49): 16966–16978.
Prasantha BR, Reichmuth C, Adler C, Felgentreu D, 2015. Lipid adsorption of diatomaceous earths and increased water permeability in the epicuticle layer of the cowpea weevil Callosobruchus maculatus (F.) and the bean weevil Acanthoscelides obtectus (Say) (Chrysomelidae). Journal of Stored Products Research 64: 36–41.
Rajendran S, 2020. Insect pest management in stored products. Outlooks on Pest Management 31(1): 24–35.
Ragaei M, Sabry AH, 2014. Nanotechnology for insect pest control. International Journal of Science & Environmental Technology 3(2): 528–545.
Remya R, 2007. Evaluation of pulse genotypes and efficacy of diatomaceous earth against different life stages of Callosobruchus chinensis (L.). MSc. Thesis, Department of Agricultural Entomology, College of Agriculture, Dharwad University of Agricultural Sciences, Dharwad.
Singh RP, Shukla VK, Yadav RS, Sharma PK, Singh PK, et al., 2011. Biological approach of Zinc oxide nanoparticles formation and its characterization. Advance Material Letters 2(4): 313–317.
Sabbour MM, 2012. Entomotoxicity assay of two nanoparticle materials 1- (Al2O3 and TiO2) against Sitophilus oryzae under laboratory and store conditions in Egypt. Journal of Novel Applied Science 1(4): 103–108.
SAS Institute, 2003. The SAS system for Windows, Release 9.0. SAS, Institute, Cary, NC.
Siddique MA, Sagheer M, Sahi ST, 2021. Comparative toxic effects of Eucalyptus globulus L. (Myrtales: Myrtaceae) and its green synthesized zinc oxide nanoparticles (ZnO NPs) against Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae). International Journal of Tropical Insect Science 42(2): 1697–1706.
Sinha K, Ghosh J, Sil PC, 2017. New pesticides: a cutting-edge view of contributions from nanotechnology for the development of sustainable agricultural pest control, In: Grumezescu AM (Ed.). New Pesticides and Soil Sensors. Academic Press. Pp. 47–79.
Shekhar S, Sharma S, Kumar A, Taneja A, Sharma B, 2021. The framework of nanopesticides: a paradigm in biodiversity. Materials Advances 2(20): 6569–6588.
Tengey TK, Alidu SM, Moro A, Nboyine J, Affram EI, 2023. Resistance to Callosobruchus maculatus among cowpea (Vigna unguiculata (L.) Walp) genotypes. Journal of Crop Improvement 37(3): 309–322.
Ulrichs C, Entenmann S, Goswami A, Mewis I, 2006. Abrasive and hydrophilic/lipophilic effects of different inert dusts used as insecticide against the stored insect pest Sitophilus granarius L. Gesunde Pflanzen 58: 173–181.
Usman M, Ayoub A, Mustafa G, Rasheed K, Nadeem MK, et al., 2022. Nanotechnology: A new technology in insect and disease control. Annals of the Romanian Society for Cell Biology 26(10): 1605–1615.
Valsalam S, Agastian P, Arasu MV, Al-Dhabi NA, Ghilan AKM, et al., 2019. Rapid biosynthesis and characterization of silver nanoparticles from the leaf extract of Tropaeolum majus L. and its enhanced in-vitro antibacterial, antifungal, antioxidant and anticancer properties. Journal of Photochemistry & Photobiology B: Biology 191: 65–74.
Vani C, Brindhaa U, 2013. Silica nanoparticles as nanocides against Corcyra cephalonica (S.), the stored grain pest International Journal of Pharma & Bio Sciences 4(3): 1108–1118.
Vijaya Sankar M, Abideen S, 2015. Pesticidal effect of green synthesized silver and lead nanoparticles using Avicennia marina against grain storage pest Sitophilus oryzae. International Journal of Nanomaterials & Biostructure 5(3): 32–39.
Vijayaram S, Razafindralambo H, Sun YZ, Vasantharaj S, Ghafarifarsani H, et al., 2023. Applications of green synthesized metal nanoparticles-A review. Biological Trace Element Research 202: 360–386.
Wakil W, Riasat T, Lord JC, 2013. Effects of combined thiamethoxam and diatomaceous earth on mortality and progeny production of four Pakistani populations of Rhyzopertha dominica (Coleoptera: Bostrichidae) on wheat, rice and maize. Journal of Stored Products Research 52: 28–35.
Wakil W, Kavallieratos NG, Usman M, Gulzar S, El-Shafie HA, 2021. Detection of phosphine resistance in field populations of four key stored-grain insect pests in Pakistan. Insects 12(4): 288.
Ying S, Guan Z, Ofoegbu PC, Clubb P, Rico C, et al., 2022. Green synthesis of nanoparticles: Current developments and limitations. Environmental Technology & Innovation 26: 102336.
Yusuf AU, Dike MC, Adebitan SA, Ahmed BI, 2011. Comparative efficacy of seven plant products on the cowpea burchid, Callosobruchus maculatus F. development and damage. Journal of Biopesticides 4(1): 19.
Zar JH, 1999. Biostatistical analysis. 4th edition. Upper Saddle River: Prentice Hall. 663 pp.
Zhao X, Zhou L, Riaz Rajoka MS, Yan L, Jiang C, et al., 2018. Fungal silver nanoparticles:  synthesis, application and challenges. Critical Reviews in Biotechnology 38(6):  817–835.
پژوهش‌های کاربردی در گیاه‌پزشکی
دوره 15، شماره 2
تیر 1405
صفحه 107-120

فایل ها

سابقه مقاله

  • تاریخ دریافت: 21 فروردین 1405
  • تاریخ بازنگری: 30 تیر 1405
  • تاریخ پذیرش: 01 مرداد 1405

هم رسانی

ارجاع به این مقاله

آمار