نوع مقاله : مقاله پژوهشی
نویسندگان
گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
One of the most significant biological threats to wheat (Triticum aestivum L.) is yellow rust disease, caused by the fungus Puccinia striiformis f. sp. tritici. To manage this disease and ensure the sustainability of wheat yield, the fundamental strategy focuses on identifying and effectively utilizing resistance genes against this disease in wheat. Considering the prevailing drought conditions in Iran and the importance of drought tolerance, the current research focused on a near-isogenic line derived from the Kalheydari wheat cultivar, a winter variety and drought-tolerant variety in western Iran. The main objective was to enhance the resistance of this line against the disease through the transfer of two resistance genes, Yr18 and Yr29, respectively from the standard donor parents Lalbahadur/Pavon and Opata 85, using the backcrossing method with the aid of molecular markers and gene pyramiding. To facilitate the selection process and increase the accuracy and possibility of simultaneous tracking of target alleles, specific molecular markers were used. Based on molecular experimental results, the co-dominant inheritance pattern of the transferred genes allowed for clear differentiation of homozygous susceptible, heterozygous, and homozygous resistant genotypes in the breeding populations. In the final evaluation, the transfer of the two genes Yr18 and Yr29 into the new wheat line derived from the Kalheydari cultivar was confirmed. This finding demonstrates the utility of molecular markers as an important tool in accelerating and successfully transferring desirable traits to wheat in breeding programs.
کلیدواژهها [English]
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