نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ولی عصر (عج)، رفسنجان، ایران
2 مرکز تحقیقات پسته، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، رفسنجان، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
The fungus Fusarium fujikuroi L. is recognized as a primary natural source of bioactive gibberellic acid hormone production. The present study investigates the effects of organic nitrogen sources and inorganic carbon at different moisture levels on the production of gibberellic acid by isolate 134 of this fungus, isolated from North Khorasan province, Iran. The optimization of gibberellic acid production was carried out in two culture systems, solid and submerged, and through successive screening steps. Qualitative evaluation of the metabolite was carried out using spectroscopic methods. In solid culture, four organic substrates were investigated, with soybean meal yielding an average production of 16.66 mg/g, significantly outperforming the others due to its high protein content and good degradability. The other three substrates (citrus pulp, rice bran, and wheat bran) were not statistically significantly different. Also, the effect of initial moisture content (5, 10, 15, and 20 mL) showed that 10 mL of water was the most optimal level. In submerged culture, among the four carbon sources, including glycerol, glucose, mannitol, and molasses, mannitol was the most effective, followed by molasses, glycerol, and glucose, respectively. This study provides practical and cost-effective solutions for the industrial production of gibberellic acid by identifying the best isolate (134), inexpensive substrate (soybean meal), carbon source (mannitol), and optimal moisture conditions (10 mL). Increasing the yield of the superior strain by using genetic methods, developing the production process on a semi-industrial scale through simultaneous optimization of key factors and the use of a bioreactor, evaluating the effectiveness of the final product on target plants, and ensuring the biosafety of the industrialized strain are among the proposed solutions for future research.
کلیدواژهها [English]
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