اثرات ضدقارچی و القاکننده مقاومت نانوذرات گرافن اکسید احیاشده با نقره علیه Pythium aphanidermatum در خیار

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه تولید و زنتیک گیاهی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

چکیده

در این مطالعه سنتز نانوذرات اکسید گرافن احیا شده با نقره (Ag-rGO)، فعالیت ضد قارچی مستقیم Ag-rGO علیه Pythium aphanidermatum  و اثربخشی آنها در القای مقاومت در برابر مرگ گیاهچه در خیار ناشی از aphanidermatum P.  بررسی شد. فعالیت ضد قارچی با استفاده از غلظت 100 میلی‌گرم در لیتر Ag-rGO ارزیابی شد که مهار قابل توجهی در شرایط آزمایشگاهی در رشد P. aphanidermatum (حدود 90٪ کاهش در مقایسه با شاهد تیمار نشده) نشان داد. آزمایش‌های گلخانه‌ای و مزرعه‌ای کاهش بروز مرگ گیاهچه در گیاهان خیار تیمار شده با Ag-rGO (حدود 75-80٪ کاهش، در مقایسه با شاهد تیمار نشده) را نشان داد. علاوه بر این، تجزیه و تحلیل واکنش زنجیره‌ای پلی‌مراز در زمان واقعی (qRT-PCR) نشان داد که تیمار Ag-rGO بیان ژن‌های مرتبط با دفاع درگیر در مسیرهای سیگنالینگ اسید سالیسیلیک (SA) و اسید جاسمونیک/اتیلن (JA/ET) را تا 10 برابر (در مقایسه با شاهد تیمار نشده) افزایش می‌دهد. در نهایت، این مطالعه اولین گزارشی است که پتانسیل Ag-rGO را به عنوان یک استراتژی جدید برای کنترل P. aphanidermatum در خیار نشان می‌دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Antifungal and resistance-inducing effects of silver-reduced graphene oxide nanoparticles against Pythium aphanidermatum in cucumber

نویسندگان [English]

  • Hossein Vasighi Meraji
  • Sonbol Nazeri
Department of Plant Production and Genetics, University of Bu-Ali-Sina, Hamedan, Iran
چکیده [English]

In the present study, silver-reduced graphene oxide nanoparticles (Ag-rGO) were synthesized, and their direct antifungal activity against Pythium aphanidermatum was evaluated, as well as their effectiveness in inducing resistance against damping-off in cucumber caused by P. aphanidermatum. The antifungal activity tests showed effective in vitro inhibition of P. aphanidermatum growth by Ag-rGO treatment at a concentration of 100 mg/L, with approximately 90% inhibition compared with the untreated control. Similar results were observed in the greenhouse and field experiments, with approximately 75–80% reduction in damping-off in cucumber plants treated with Ag-rGO (compared to untreated controls). Expression level of defense-related gene expression in SA and JA/ET signaling pathways was increased by 10-fold by treatment with Ag-rGO compared to untreated controls when determined by real-time RT-PCR assay(qRT-PCR). These results are the first to report that Ag-rGO could be a novel strategy for controlling P. aphanidermatum.

کلیدواژه‌ها [English]

  • Defense -related genes
  • Phytium aphanidermatum
  • Systemic acquired resistance
  • QRT-PCR analysis
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