گزارش‌های جدید از گونه‌های قارچی همراه حشرات در ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه‏‌‌‌‌‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، ایران.

2 گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، ایران. مرکز تحقیقات بیوتکنولوژی و علوم زیستی، دانشگاه شهید چمران اهواز، ایران.

چکیده

چکیده
قارچ‌های بیماری‌زا و غیربیماریزای موجود در بدن حشرات می‌توانند روی زیست‌شناسی و جمعیت آن‌ها اثر بگذارند. در طول سال‏های 1397-1396 با هدف شناسایی قارچ‌های همراه حشرات، مراحل زیستی شامل لارو، شفیره و حشرات کامل (زنده یا مرده و سالم) از زیستگاه‌های متنوع در استان ‏های خوزستان و گلستان جمع‌آوری و قارچ‌های همراه آن بعد از ضدغفونی سطحی جداسازی شدند. در این بررسی، 13 جدایه متنوع انتخاب (بر اساس ریخت‌شناسی اولیه) و بر اساس ویژگی‌های ریخت ‏شناختی و مولکولی تشخیص داده شدند. برای تشخیص مولکولی، توالی‌های ITS-nrDNA، tef1α، tub2و gapdh در جایی که نیاز بود، بدست آمد. بر این اساس 12 گونه شامل Auxarthron kuehnii، Biscogniauxia sp.، Cladosporium halotolerans، Curvularia mosaddeghii، Fusarium brachygibbosum، Lasiodiplodia mediterranea،Metarhizium brunneum، Nigrospora gorlenkoana، N. oryzae،  N. osmanthi، Petriella guttulata و Phascolomyces articulosus شناسایی شدند. در این مطالعه، جنس‌های Auxarthron (A. kuehnii) و Phascolomyces (P. articulosus) و گونه‌های L. mediterranea، N. gorlenkoana و N. osmanthi برای اولین بار از ایران گزارش می‌شوند.
 

کلیدواژه‌ها


عنوان مقاله [English]

New records of the fungal species associated with insects in Iran

نویسندگان [English]

  • Hamideh Aghyl 1
  • Mehdi Mehrabi-Koushki 2
  • Mehdi Esfandiari 1
1 Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran.
2 Departement of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran.Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Iran.
چکیده [English]

Summary
Pathogenic and non-pathogenic fungi growing on insect’s body cansignificantly affect their biology and population. To identify insect-associated fungi, the life stages of the insects, including larva, pupa and adults (alive or dead and helthy), were collected from different habitats in Golestan and Khuzestan Provinces during 2017-2018 and their associated fungi were isolated after surface-sterilization. In this study, 13 various isolates were selected (based on primary morphology) and identified according to morphological and molecular analysis. For molecular identification, the sequences of ITS-nrDNA, tef1a, tub2 and gapdh were obtained, where required for identification. Accordingly, eleven species were identified as follow: Auxarthron kuehnii, Biscogniauxia sp., Cladosporium halotolerans, Curvularia mosaddeghii, Fusarium brachygibbosum, Lasiodiplodia mediterranea, Metarhizium brunneum, Nigrospora gorlenkoana, N. oryzae, N. osmanthi, Petriella guttulata and Phascolomyces articulosus. This study firstly reports the genera of Auxarthron (A. kuehnii) and Phascolomyces (P. articulosus) and the species of L. mediterranea, N. gorlenkoana and N. osmanthi for the mycobiota of Iran. 

کلیدواژه‌ها [English]

  • Keywords: Ascomycetes
  • Biodiversity
  • Enthomopathogens
  • Fungus
  • Molecular phylogeny
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