Efficacy of Entomopathogenic Nematode Heterorhabditis bacteriophora asBiological Control of Root Knot Nematode Meloidogyne javanicaUnder Laboratory Conditions

Document Type : Research Paper

Authors

1 Former MSc. Student of Plant Pathology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Associate Professor, Dept of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

3 Professor, Dept of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Abstract

Antagonism of entomopathogenic nematodes (EPNs) on plant – parasitic nematodes (PPNs) has gained interest over the recent years. Therefore, the efficacy of live and dead infective juveniles of entomopathogenic nematode, Heterorhabditis bacteriophora Poinarwith concentrations of 5, 10, 25, 50 and 100 IJs/ml in 24 well plates was evaluated on egg hatching inhibition and second stage juveniles (J2) mortality of the root knot nematode Meloidogyne javanica Treub in concentrations of 50 eggs, 50 larvae and a combination of 50 eggs and larvae. The experimental design was split plot with six treatments and four replications. The whole experiments were replicated twice. The data recorded after 48, 72, 96 and 120 hours showed that both live and dead entomopathogenic nematodes have significant difference on root knot second stage juveniles (J2) mortality and egg hatching inhibition. The highest mortality was achieved in 100 IJs/ml and after 120 hours and the most inhibition of hatching in concentration of 100 IJs/ml and after 48 hours. Concerning the mixture of 50 eggs and larvae, the results indicated that the effect of live juveniles of H. bacteriophora on second stage juveniles (J2) mortality have no significant difference among the various time treatments.

Keywords


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