Biocontrol of fusarium crown and root rot of cucumber using selected endophytic bacteria under laboratory and greenhouse conditions

Articles in Press

Document Type : Research Paper

Authors

1 Department of Plant Protection, Agriculture Faculty, Kurdisan University, Sanandaj, Iran

2 Department of Plant Protection, Safiabad Agricultural Research and Education and Natural Resources center, Dezful. Iran

10.22034/arpp.2025.20880

Abstract

Fusarium crown and root rot is one of the most important diseases affecting cucumber crops worldwide. In this study, the inhibitory potential of six selected endophytic bacterial strains including, Staphylococcus sciuri (MarR44), Serratia rubidaea (Mar61-01), Bacillus atrophaeus (DM6120), Bacillus sp. (MarG19), Bacillus sp. (MarH51), and Bacillus sp. (TN16) was evaluated to control this disease in cucumber, under laboratory and greenhouse conditions following a completely randomized design. Under laboratory conditions, the inhibitory effects of these six strains were significant compared to the control in dual-culture, volatile metabolites, non-volatile metabolites, and spore germination assays. In each assay, the bacterial strains S. sciuri (MarR44) with 56%, S. rubidaea (Mar61-01) with 75.8%, S. rubidaea (Mar61-01) with 11%, and Bacillus sp. (MarH51) with 76.53% exhibited the highest suppression of mycelial growth and spore germination of the pathogen, respectively. Greenhouse experiments using two methods of seed treatment and soil inoculation with endophytic bacterial strains in pathogen-infested soil demonstrated that the endophytic bacterial strains reduced disease incidence by over 60%. Furthermore, the bacterial strains significantly increased all growth parameters even in the presence of the pathogen as compared to control treatment in both methods. The findings indicated that the endophytic bacterial strains in this study possess considerable potential for suppressing Fusarium crown and root rot in cucumber. 

Keywords

Journal of Applied Research in Plant Protection

Articles in Press, Accepted Manuscript
Available Online from 03 December 2025

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History

  • Receive Date: 13 June 2025
  • Revise Date: 28 August 2025
  • Accept Date: 27 September 2025

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