Biology of Brevicoryne brassicae and its parasitoid wasp Diaeretiella rapae on canola inoculated with Bacillus subtilis, in the laboratory conditions

Document Type : Research Paper

Authors

1 Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran.

2 Department of Production Engineering and Plant Genetics, College of Agriculture, Razi University, Kermanshah, Iran.

Abstract

Plant probiotic bacteria have been found to induce systemic plant defense and affect the interactions between plants and insects across different trophic levels. In this research, the effect of a probiotic bacterium, Bacillus subtilis (strain S) on life table parameters of Brevicoryne brassicae, and its parasitoid wasp, Diaeretiella rapae, on canola was investigated. The results showed that the S strain significantly reduced the intrinsic rate of increase (r) of the cabbage aphid. The values of the intrinsic rate of increase (r) were 0.302 ± 0.012 and 0.237 ± 0.007 (day-1) for control and plant probiotic bacteria-treatments, respectively, while, net reproductive rates (R0) were 16.60 ± 1.90, and 33.71 ± 2.60 (offspring/individual), respectively. The study on the biology of parasitoid wasp showed no significant differences in various life table parameters, except for the mean generation time (T) between control and bacteria-treatments. Additionally, the gas exchange was measured on the control and canola treated with the various strains of B. sabtilis including S, GBO3, INR7, AS, and BS. The results indicated that the highest and lowest photosynthetic rates were calculated in S and AS, with rates of 21.66 and 5.91 µmol (Co2)/m2/S, respectively. Based on our findings, the S strain of B. subtilis, by reducing the aphid's capacity to increase its population, can be used as an effective agent in the integrated management of the cabbage aphid.

Keywords


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