Monitoring the virulence factors of Puccinia triticina, the causal agent of wheat leaf rust, in Iran during the cropping years 2022-2023

Document Type : Research Paper

Authors

1 Department of Cereal Research, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Crop and Horticultural Science Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Moghan, Iran

3 3- Crop and Horticultural Science Research Department, Khuzestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran.

4 4- Crop and Horticultural Science Research Department, Safiabad Dezful Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Dezful, Iran.

5 5- Crop and Horticultural Science Research Department, Ardebil Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Ardebil, Iran.

6 Crop and Horticultural Science Research Department, Golestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Gorgan, Iran.

7 Department of Cereal Research, Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

8 Crop and Horticultural Science Research Department, Safiabad Dezful Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Dezful, Iran.

9 7- Crop and Horticultural Science Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Sari, Iran.

10 8- Crop and Horticultural Science Research Department, Lorestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Boroujerd, Iran.

11 Plant Protection Research Department, Kermanshah Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Kermanshah, Iran.

Abstract

Wheat leaf rust caused by Puccinia triticina is the most important wheat disease in terms of its wide distribution and yield loss in the world. To investigate the virulence factors of P. triticina in Iran, the present research was carried out in several locations including Karaj, Gorgan, Sari (Ghaemshahr), Kelardasht, Ardabil, Moghan, Boroujerd, Ahvaz, Dezfoul, and Kermanshah. In this research, 39 Near isogenic lines (NILs) developed from the wheat cultivar Thatcher and each carrying a specific leaf rust resistance gene/s were examined under natural disease infection during the two years 2022-2023. At the full opening of flag leaf stage and after uniformity in disease emergence on the susceptible cultivar, disease severity and infection type were scored based on the modified Cobb scale and Rolfes et al. respectively. In this study, reactions of 50S and higher were recorded as virulence for the contaminated lines. Based on the isogenic lines reactions in different locations in 2022, no virulence was observed on Lr1, Lr2a, Lr3ka, Lr10, Lr11, Lr12, Lr14b, Lr17, Lr18, Lr19, Lr20, Lr21, Lr22a, Lr29, Lr32, Lr33, Lr34 and the gene Lr37 and in 2023 no virulence was observed on Lr9, Lr17, Lr18, Lr19, Lr22a, Lr29 and the Lr30. Based on two-year results of isogenic lines in different locations, no virulence was observed on the genes Lr17, Lr18, Lr19, Lr22a and Lr29 gene combination in the study areas during the two years of isogenic lines and then identified as effective genes for wheat rust disease. Virulence was observed on plants carrying other resistance gene/s, at least in one location tested, indicating ineffectiveness of the gene/s to leaf rust in that location.

Keywords

References

Afshari F, 2000. Studies on rust resistance in wheat with particular emphasis on stripe rust. Ph.D. Thesis, University of Sydney, Australia.
Afshari F, 2008. Identification of virulence factors of Puccinia triticina, the causal agent of wheat leaf rust in Iran. Proceedings of 11th international wheat genetic symposium, Vol 3. Sydney University Press, Sydney, Australia. Pp. 709–711..
Afshari F, Torabi M, Malihipour A, 2003. Appearance of a new race of puccinia striiformis f. sp. tritici in Iran. Seed & Plant Journal 19-4: 543–546.
Bamdadian A, 1973. Physiologic races of Puccinia recondita in Iran (1968-1972). Cereal Rust Bulletin 1: 45–48.
Dadrezaei ST, Dehghan MA, Safavi SA, Dalvand M, Shahbazi K, 2023. Resistance evaluation of advanced and commercial genotypes of Iranian wheat to leaf rust at seedling and adult plant stages. Journal of Applied Research in Plant Protection 11: 1–13 (In Persian with English abstract).
Dadrezaei ST, Dehghan MA, Safavi SA, Dalvand M, Shahbazi K, et al., 2021. Study of virulence factors of causal agent of wheat leaf rust (Puccinia triticina Eriks.) in Iran during 2017-2021. Seed & Plant Journal 37: 149-169 (In Persian with English abstract).
Dadrezaei ST, Delfan S, Allahassani E, 2022. Identification of the pathotypes and physiologic races of Puccinia triticina, the causal agent of wheat leaf rust in Iran. Journal of Applied Research in Plant Protection 11: 1–15 (In Persian with English abstract).
Dadrezaei ST, Mohammadi Goltappeh E, Afshari F, Nazari K, 2012. Pathotypes and physiologic races of Puccinia triticina Erik's. The causal agent of wheat leaf rust and their distribution in Iran in 2009 and 2010. Seed & Plant Improvement Journal 28(1): 685–715 (In Persian with English abstract).
Dadrezaei ST, Nazari K, 2015. Detection of wheat rust resistance genes in some of the Iranian wheat genotypes by molecular markers. Seed & Plant Improvement Journal 31(1): 163–187 (In Persian with English abstract).
Dadrezaei ST, Nazari K, Afshari F, Torabi M, 2017. Genetic Diversity and Migration of Wheat Leaf Rust Populations in Iran Based on Virulence and Molecular Data. Seed & Plant Improvement Journal 33 (1): 401–425 (In Persian with English abstract).
Dyck PL, Johnson R, 1983. Temperature sensitivity of genes for resistance in wheat to Puccinia recondita. Canadian Journal of Plant Pathology 5: 229–234.
Dyck PL, Samborski DJ, 1968. Genetic of resistance to leaf rust in the common wheat varieties, Webster, Loros Brevit, Carina, Malakof and Centenario. Canadian Journal of Genetics & Cytology 10: 7–17.
El-Orabey W, Shaheen D, Mabrouk O, Elkot A, Esmail S, 2020. Effect of temperature on monogenic lines of wheat leaf rust caused by Puccinia triticina. Egyptian Journal of Agronomy 42: 263–277.
Hovmøller MS, Walter S, Bayles RA, Hubbard A, Flath K, et al., 2016. Replacement of the European wheat yellow rust population by new races from the centre of diversity in the near-Himalayan region. Plant Pathology 65: 402–411.
Huerta-Espino J, Singh R, Crespo-Herrera LA, Villaseñor-Mir HE, Rodriguez-Garcia MF, et al., 2020. Adult plant slow rusting genes confer high levels of resistance to rusts in bread wheat cultivars from Mexico. Frontiers in Plant Science 11: 1–15.
Huerta-Espino J, Singh RP, Germa´n, S, McCallum BD, Park RF, et al., 2011. Global status of wheat leaf rust caused by Puccinia triticina. Euphytica 179:143–160.
Johnston CO, Heyne EG, 1964. Wichita back- cross lines for differential hosts inidentifying physiologic races of Puccinia recondita. Phytopathology 54: 385–388.
Kaul K, Shaner G, 1989. Effect of temperature on adult-plant resistance to leaf rust in wheat. Phytopathology 79: 391–394.
Kolmer JA, 2013. Leaf rust of wheat: pathogen biology, variation and host resistance. Forests 4 (1): 70–84.‏
Kolmer JA, 2019. Virulence of Puccinia triticina, the wheat leaf rust fungus, in the United States in 2017. Plant Disease 103: 2113–2120.
Kolmer JA, 2001. Molecular polymorphism and virulence phenotypes of the wheat leaf rust fungus Puccinia triticina in Canada. Canadian Journal of Botany 79: 917–926.
Kolmer JA, Ordoñez ME, 2007. Genetic differentiation of Puccinia triticina populations in Central Asia and the Caucasus. Phytopathology 97:1141–1149.
Kumar K, Jan I, Saripalli G, Sharma PK, Mir RR, Balyan HS, Gupta PK, 2022. An update on resistance genes and their use in the development of leaf rust resistant cultivars in wheat. Frontiers in Genetics. 13: 816057.
Mahdian S, Torabi M, Alizadeh A, 1999. Avirulence.virulence factors in Puccinia recondita f. sp. tritici isolates from different parts of Iran. Seed & Plant 15: 56–67 (In Persian with English abstract).
Malihipour A, Najafian G, 2023. Evaluation of the moderate agro-climate zone elite wheat lines of Iran in terms of reaction to stem rust. Journal of Applied Research in Plant Protection 12 (4): 425–437.
McIntosh RA, 1988. The role of specific genes in breeding for durable stem rust resistance in wheat and triticale. Simmonds, NW, Rajaram, S. (Eds). Breeding strategies for resistance to rusts of wheat. CIMMYT, Mexico. D.F. Pp. 1–9.
McIntosh RA, Brown GN, 1997. Anticipatory breeding for resistance to rust diseases in wheat. Annual Review of Phytopathology 35: 311–326.
McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, et al., 2014. Catalogue of gene Symbols for Wheat: 2013-2014 Suplement. http://maswheat.ucdavis.edu/CGSW/2013- Supplement.pd.
Morgounov A, Rosseeva L, Koyshibayev M, 2007. Leaf rust of spring wheat in Northern Kazakhstan and Siberia: Incidence, virulence, and breeding for resistance. Australian Journal of Agricultural Research 58: 847–853.
Niazmand AR, Afshari F, Abbasi M, Rezaee S, 2010. Study on pathotypes diversity and virulence factors of Puccinia triticina Eriksson, the causal agent of wheat brown rust in Iran. Iranian Journal of Plant Pathology 46: 187–202 (In Persian with English abstract).
Ordoñez ME, Germán SE, Kolmer JA, 2010. Genetic differentiation within the Puccinia triticina population in South America and comparison with the North American population suggests common ancestry and intercontinental migration. Phytopathology 100: 376–383.
Park RF, Burdon JJ, McIntosh RA, 1995. Studies on the origin, spread, and evolution of an important group of Puccinia recondita f. sp. tritici pathotypes in Australia. European Journal of Plant Pathology 101: 613–622.
Park RF, Felsenstein FG, 1995. Physiologic specialization and pathotype distribution of Puccinia recondita in Western Europe. Plant Pathology 47: 157–164.
Peterson RF, Campbell AB, Hannah AE, 1948. A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Canadian Jourmal of Research 26: 496–500.
Qureshi N, Bariana H, Kolmer JA, Miah H. Bansal U, 2017. Genetic and Molecular Characterization of Leaf Rust Resistance in Two Durum Wheat Landraces. Phytopathology 107:1381–1387.
Roelfs AP, Singh RP, Saari EE, 1992. Rust Disease of Wheat: Concepts and Methods of Disease Management. CIMMYT, Mexico, D.F., 81 pp.
Singh RP, 1993. Resistance to leaf rust in 26 Mexican wheat cultivars. Crop Science 33:633–637.
Torabi M, Mardoukhi V, Froutan A, Aliramaei M, Dadrezaie ST, et al., 2003. Virulence genes of Puccinia recondita f. sp. tritici, the causal agent of wheat leaf rust in some regions of Iran during 1995-1999. Seed & Plant Improvement Journal 18: 432–449 (In Persian with English abstract).
Torabi M, Nazari K, Afshari F, 2001. Genetic of pathogenicity of Puccinia recondita f. sp. tritici, the causal agent of leaf rust of wheat, Iranian Journal of Agriculture Science 32: 625–635 (In Persian with English abstract).
Zhang L, Shi C, Li L, Li M, Meng Q, et al., 2020. Race and virulence analysis of Puccinia triticina in China in 2014 and 2015. Plant Disease 104: 455-4.
 
Journal of Applied Research in Plant Protection
Volume 13, Issue 3
September 2024
Pages 319-331

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History

  • Receive Date: 20 December 2023
  • Revise Date: 27 April 2024
  • Accept Date: 28 April 2024

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