The report of Grapevine virus A from the vineyards of Khorasan Razavi Province and the phylogenetic analysis of the virus based on a portion of the ORF5 gene

Document Type : Research Paper

Authors

1 Plant Protection Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran.

2 Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.

Abstract

Grapevine virus A (GVA) belongs to Vitivirus genus and is one of the most important causes of wood rugose in grapes. During the years 2019 to 2021, a total of 79 leaf samples showing symptoms of leaf yellowing and reddening from vineyards of Khorasan Razavi Province (including Bardaskan, Kashmar, Khalilabad, and Mohammadiyeh) were collected. The initial infection of the samples to GVA was directly checked using an ELISA test, and the final confirmation of results was performed by RT-PCR with specific primers. Total RNA extraction was performed from scraped bark of young stems using CTAB method. GVA was detected in 14 samples through the ELISA test, and a fragment of 238 base pairs from the p10 protein was amplified in these 14 samples using RT-PCR. The amplified products from 4 samples, were ligated into the pTG19-T vector, and the recombinant plasmids were sequenced bidirectionally. The obtained sequences were compared with the other GVA sequences in GenBank using BlastN tool and the nucleotide similarity was 85.7-95%. The phylogenetic tree based on p10 protein showed there are four main groups, and Khorasan Razavi isolates classified within the fourth group. Comparison of p10-protein sequence of Iranian isolates with other isolates showed there is a conserved region in N-terminal region, which includes an arginine-rich motif followed by a zinc-finger motif. There have been many studies on other grape viruses in this province, but this is the first report of investigating the phylogenetic analysis of GVA isolates in the vineyards of Khorasan Razavi province.

Keywords

Journal of Applied Research in Plant Protection

Articles in Press, Accepted Manuscript
Available Online from 22 November 2023

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History

  • Receive Date: 22 July 2023
  • Revise Date: 05 September 2023
  • Accept Date: 20 September 2023

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