Evaluation of the reaction of some cantaloupe and watermelon inbred lines to Watermelon mosaic virus under greenhouse conditions

Document Type : Research Paper

Authors

1 Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

2 2 Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

4 Department of Engineering of Horticultural Scince, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

Watermelon mosaic virus (WMV) is one of the most important viruses of cucurbit plants. In this research, the reaction of 10 cantaloupe and eight watermelon Ajili (Jabani) inbred lines was evaluated under greenhouse conditions against a WMV isolate. Results indicated that only the inbred line of cantaloupe selfing 2 showed a delay in symptoms development which attributed to low disease severity index and average absorbance in ELISA test. Therefore, it was considered as resistant inbred line to the virus. While the inbred lines 3, 5, 6 and 8 were moderately susceptible and 4, 7, 10 and 11 were susceptible to the virus. Among watermelon inbred lines, 2 and 5 were found to be susceptible, whereas other accessions did not show any disease symptoms and also their severity index were recorded to be zero. Furthermore, results of enzyme-linked immunosorbent assay (ELISA) and RT-PCR tests showed that the later accessions are resistant to WMV. To assess the virus's impact on plant biomass, cantaloupe and watermelon inbred lines were mechanically inoculated with the WMV. Results indicated that the susceptible cantaloupes and watermelons against WMV were found to have significantly lower fresh leaves, dry stems and biomass than control plants (non-inoculated). Collectively, the cantaloupe inbred lines 1 and 2 and watermelon inbred lines 1, 3, 6, 7, 8 and 11 used in this study are probably resistant against WMV and can be recommended in breeding programs to establish more resistant crops.

Keywords

References

Agrawal H, Purohit AN, Upadha MD, 1979. Effect of time of inoculation and light conditions on the susceptibility of Capsicum pendulum to potato virus X. Proceedings of the Indian Academy of Sciences-Section B. Part2, Plant Sciences 88(1): 75–77.
Alinizi HR. Mehrvar M. Zakiagh M, 2020. Genetic diversity and biological characterization of watermelon mosaic virus isolates from Iran and Iraq. Acta Virologica 64(4): 506–508.
Arzani A, Ahoonmanesh A, 2000. Study of resistance to cucumber mosaic virus, watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars. Iranian Agricultural Research 19: 129–144 (In Persian with English abstract).
Ashfaq M, Iqbal S, Mukhtar T, Shah H, 2014. Screening for resistance to cucumber mosaic cucumovirus in chili pepper. The Journal of Animal & Plant Sciences 24 (3): 791–795.
Banimostafa A, Keshavarz T, Maleki M, 2017. Evaluation of resistance to cucurbit yellow stunting disorder virus in cucumber accessions. Plant Protection (Scientific Journal of Agriculture) 40 (4): 87–95.
Bender FE, 2020. Statistical methods for food and agriculture. CRC Press.
Booker HM, Umaharan P, McDavid CR, 2005. Effect of Cowpea severe mosaic virus on crop growth characteristics and yield of cowpea. Plant Disease 89: 515–520.
Cooper JI, Jones AT, 1983. Responses of plants to viruses: proposals for the use of terms Phytopathology 73: 127–128.
Daryono BS, Somowiyargo S, Natsuaki KT, 2003. New source of resistance to Cucumber mosaic virus in melon. SABRAO Journal of Breeding & Genetics 35: 19–26.
Ebrahim-Nesbat F, 1972. Distribution of watermelon mosaic viruses 1 and 2 in Iran. Phytopathology 79: 352–358.
Esmaeili M, Heydarnejad J, 2016. Evaluation of reaction of watermelon cultivars to watermelon chlorotic stunt virus by agroinoculation with an infectious clone of the virus. Iranian Journal of Plant Pathology 52 (1): 99–107 (In Persian with English abstract).
Fischer HU, Lockhart BE, 1974. Serious losses in cucurbits caused by Watermelon mosaic virus in Morocco. Plant Disease 58: 143–146.
Gilbert RZ, Kyle MM, Munger HM, Gray SM, 1994. Inheritance of resistance to Watermelon mosaic virus in Cucumis melo L. Hortscience 29: 107–110.
Jafari M, Shams-bakhsh M, Moieni A, 2016. Reaction of commercial canola varieties and lines against turnip mosaic virus (TuMV) isolate. Iranian Journal of Plant Pathology 52 (2): 147–152 (In Persian with English abstract).
Keshavarz T, Shams-bakhsh M, Izadpanah K, 2013. Evaluation of reaction of some Iranian melon accessions to cucurbit yellow stunting disorder virus. Iranian Journal Plant Pathology 49 (2): 69–71 (In Persian with English abstract).
Kimmins WC, 1967. The effect of darkening on the susceptibility of French bean to tobacco necrosis virus. Canadian Journal of Botany 45(5): 543–553.
Luis-Arteaga M, Alvarez JM, Alonso-Prados JL, Bernal JJ, García-Arenal F, Laviña A, Batlle A, Moriones E, 1998. Occurrence, distribution, and relative incidence of mosaic viruses infecting field-grown melon in Spain. Plant Disease 82: 979–982.
Martín-Hernández AM, Picó B, 2021. Natural resistances to viruses in cucurbits. Agronomy 11 (23): 1–29.
Massumi H, Mohammadi F, Salari KH, Heydarnejad J, Hosseinipour A, 2016. Nucleotide sequence and phylogenetic analysis of coat protein gene of two Iranian Watermelon mosaic virus isolates. Journal of Applied Research in Plant Protection 6 (1): 27–40 (In Persian with English abstract).
Mirhosseini-chahooki SZ, Massumi H, Heydanejada J, Hosseinipour A, Abdoshahi R, Maddahian M, 2022. The reaction of forty cucurbits genotypes against     Cucumber mosaic virus. Plant Pathology Science 11(1): 48–59.
Mowat WP. Dawson S, 1987. Detection of plant viruses by ELISA using crude sap extracts and unfractionated antisera. Journal of Virological Methods 15: 233–247.
Nemati Z, Banihashemi Z, 2015. Reaction of cucurbit cultivars to Phytophthora melonis and P. drechsleri under greenhouse conditions. Iranian Journal of Plant Pathology 51 (3): 375–384 (In Persian with English abstract).
 Nematollahi S, Panahborhani N, Koolivand D, 2021. Molecular characterization and population evolution analysis of Watermelon mosaic virus isolates on cucurbits of Northwest Iran. 3 Biotech 11(2): 43.
Pacheco DA, Rezende JAM, Piedade SMS, 2003. Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of papaya ringspot virus. Scientia Agricola 60 (4): 691–698.
Provvidenti R, 1986. Reactions of accessions of Citrullus colocynthis from Nigeria to Zucchini yellow mosaic virus and other cucurbit viruses. Cucurbit Genetics Cooperative Report 9: 82–83.
Purcifull DE, Hiebert E, Edwardson J, 1984. Description of Plant Viruses CMI/AAB 293. 32: 307–311.
Rahimian H, Izadpanah K, 1978. Identity and prevalence of mosaic inducing cucurbit viruses in Shiraz, Iran. Journal of Phytopathology 92 (4): 305–312.
Sabouri M, Heydarnejad J, 2013. Construction and demonstration of infectivity of the infectious clone of the bipartite genome of Tomato leaf curl palampur virus-Iranian isolate. Iranian Journal of Plant Pathology 49 (4): 403–409 (In Persian with English abstract).
Salehi M, Nejat N, Masumi M, Niroomand M, Izadpanah K, 2006. Greenhouse evaluation of sugar beet germplasm for curly top resistance. Iranian Journal of Plant Pathology 42: 55–69 (In Persian with English abstract).
Samei A, Massumi H, Shaabanian M, Hosseini pour A. Heydarnejad J, 2008. Evaluation of some cucurbit cultivars grown in the fields and greenhouses to six important viruses. Journal of Agricultural Sciences and Natural Resources 15 (1): 86–96.
SAS Institute, 2004. Base SAS 9.1 procedures guide. Cary (NC): SAS Institute Inc.
Schroeder WT, Provvidenti R, 1971. A common gene for resistance to Bean yellow mosaic virus and Watermelon mosaic virus 2 in Pisum sativum. Phytopathology 61: 846–848.
Sharifi M, Massumi H, Heydarnejad J, Hosseini Pour A, Shaabanian M, Rahimian H, 2008. Analysis of the biological and molecular variability of Watermelon mosaic virus isolates from Iran. Virus Genes 37: 304–313.
Shoeibi S, Nasrollanezhad S, 2010. Detection and distribution of Watermelon mosaic virus (WMV) in cucurbits fields of Golestan province. Journal of Agricultural Sciences and Natural Resources 16 (1): 302–310
Shukla DD, Ward CW, Brunt AA, 1994. The Potyviridae, CAB International, Wallingford, UK 516 pp.
Vaisman M., Hak H, Arazi T, Spiegelman Z, 2022. The impact of tobamovirus infection on root development involves induction of auxin response factor 10a in tomato. Plant and Cell Physiology 63 (12): 1980–1993.
Weidemann HL, Mostafawy M, 1972. Watermelon mosaic virus type 2 in Iran and its transmission by different aphid species. Iranian Journal of Plant Pathology 8: 20 (In Persian with English abstract).
Yoshida K, Goto T, Nemoto M, Tsuchizaki T, 1980. Five viruses isolated from melon (Cucumis melo L.) in Hokkaido. Annals of the Phytopathological Society of Japan 46(3): 339–348.
 
Journal of Applied Research in Plant Protection
Volume 13, Issue 1
April 2024
Pages 45-57

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History

  • Receive Date: 29 May 2023
  • Revise Date: 28 August 2023
  • Accept Date: 01 September 2023

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