Detection and phylogenetic analysis of saffron latent virus in saffron fields of South Khorasan and Razavi provinces

Document Type : Research Paper

Authors

1 1Department of herbal medicine, School of Agriculture, Birjand University.

2 Department of herbal medicine, School of Agriculture, Birjand University.

3 Plant Protection Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran

Abstract

The Saffron Latent Virus (SaLV) is new species in the Potyvirus genus, which has been recently characterized from Iran. Even though most of the symptoms on infected plants remain hidden, it causes considerable damage to saffron fields. There is no data available on the phylogenetic relationship among different strains of this virus based on its coat protein, despite its high incidence and economic significance. To address this, we collected 152 samples of saffron leaves, flowers, and corms from fields in South Khorasan and Razavi Khorasan provinces, both with and without symptoms like stunting, yellowing, and twisting. After extracting RNA, the presence of SaLV was examined using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers targeting the coat protein. The findings revealed that 92 out of 152 samples (60%) were infected with the virus. Subsequently, seven representative samples, each from a selected region were sequenced. Phylogenetic analysis using MEGAX software showed that the virus strains formed two distinct groups, and the strains studied were placed in the second group. Interestingly, strains from the same geographical region and product were distributed among different groups, suggesting that geographical separation and host type did not significantly impact the viral diversity. This study is the first in detecting of SaLV in saffron flowers and corms and pinpointing the diversity of its new strains based on the coat protein in Iran.

Keywords

References

Corwin JA, Kliebenstein DJ, 2017. Quantitative resistance: More than just perception of a Pathogen. The Plant Cell 29: 655–665.
Edwardson JR, Christie RG, Ko NJ, 1984. Potyvirus cylindrical inclusions- Subdivision-IV. Phytopathology 74(9): 1111–1114.
Escribano J, Rubio A, Alvarez-Ortí M, Molina A, Fernández JA, 2000. Purification & characterization of a mannan- binding lectin specifically expressed in corms of saffron plant (Crocus sativus L.). Journal of Agricultural and Food Chemistry 48(2): 457–63.
Fernández JA, 2004. Biology. Biotechnology & Biomedicine of Saffron. Recent Research Developments in Plant Science 2: 127–159.‏
Fraile A, García-Arenal F, 2016. Environment & evolution modulate plant virus pathogenesis. Current Opinion in Virology 17: 50–56.
Grilli Caiola M, Faoro F, 2011. Latent virus infections in Crocus sativus & Crocus cartwrightianus. Phytopathol Mediterranea 50(2): 175–182.‏
Hollings M, Brunt AA, 1981. Potyvirus group. CMI/AAB Descriptions of Plant Viruses 7: 254
Hull R, 2002. Matthews’ Plant Virology. Academic Press. San Diego, USA.4: 1001.
Katoh K, Rozewicki J, Yamada KD, 2019. MAFFT online service: Multiple sequence alignment, interactive sequence choice & visualization. Briefings in Bioinformatics 20: 1160–1166.
Kumar S, Stecher G, Tamura K, 2016. MEGA7: Molecular evolutionary Genetics analysis version 7.0 for bigger datasets. Molecular Biology & Evolution 33(7): 1870–4.
Miglino R, Jodlowska A, Van schadewijk AR, 2005. First report of Narcissus mosaic virus infecting Crocus spp. cultivars in the Netherlands. Plant Disease 89(3): 342–342.‏
Molina RV, Valero M, Navarro Y, Guardiola JL, 2005. Temperature effects on flower formation in Saffron (Crocus Sativus L.). Scientia Horticulturae 103(3): 361–379.‏
Movi S, 2018. Genetic diversity of saffron latent virus (SalV) based on the P1 region & its several features, Master Thesis, University of Tehran. 163pp.
Movi S, Dizadji A, Parizad S, Zarghani SN, 2022. Biological characteristics & genetic variation analyses of Saffron latent virus (SaLV) based on genomic P1-Pro and P3 regions. European Journal of Plant Pathology 164(2): 299–312.
Muhire BM, Varsani A, Martin DP, 2014. SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PloS One 26(9): e108277.
Omid Beigi R, 2000. Production & Processing of Medicinal Plants. Astan Quds Razavi Publications 4: 159–166.
Parizad S, 2012. Identification & investigation of infectious viruses in saffron (Crocus sativus L.) cultivation regions in Iran. Master Thesis. College of Agriculture & Natural Resources, University of Tehran.
Parizad S, 2018. Description & genetic diversity of saffron pathogenic Poty virus species and investigating the effect of the virus on some metabolites Secondary host, PhD thesis, University of Tehran. 145pp.
Parizad S, Dizadji A, Kohi Habibi M, Mohammadi GM, Kalantari S, Izadpanah F, García-Arenal F, Winter S, 2016. Identification and Partial characterization of the virus infecting saffron (Crocus Sativus) in Iran. Iranian Journal of Plant Protection Science; 47(2): 1–25
Paseban F, 2006. Effective factors on exporting iranian saffron. Journal of Sustainable Growth & Development (the Economic Research) 6(2): 1–15.‏
Shukla DD, Tosic M, Jilka J, Ford RE, Toler RW, Langham MAC, 1989. Taxonomy of Potyviruses infecting maize, sorghum & sugarcane in Australia & the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology. 79(2): 223–229.
Journal of Applied Research in Plant Protection
Volume 13, Issue 1
April 2024
Pages 15-25

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History

  • Receive Date: 13 May 2023
  • Revise Date: 20 August 2023
  • Accept Date: 20 August 2023

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