Identification of some fungal species in Chaetomiaceae and Didymellaceae families associated with foliar and trunk diseases symptoms on forest trees in the Zagrosian region

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agriculture,, Shahid Chamran University of Ahvaz, Ahvaz

2 Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz

3 assistant professor, Plant protection Department, Shahid Chamran university of Ahvaz, Ahvaz, Iran

Abstract

Mountains of Shahiyoon and Salandkooh (Dezful, Khuzestan), Kabirkooh (Abdanan, Ilam) and ZazoMahroo (Aligoodarz, Lorestan) constitute small parts of the Zagrosian forests in Iran. During 2020-2022, 50 symptomatic samples from forest trees including Acer sp., Amygdalus scoparia, Crataegus sp., Pistacia atlantica, Quercus brantii and Ziziphus sp., showing leaf blight, leaf spot, fruit rot, decline and dieback, were collected. Totally, 153 fungal isolates were obtained on potato–dextrose–agar medium (PDA), of which 17 were identified at species level based on combination of morphological characteristics and sequence data of marker genes. Morphological characteristics and growth rate were evaluated on oat -meal - agar (OA) and PDA. The ITS-rDNA region (in some isolates) and part of the tub2 (in all isolates) and rpb2 (in some isolates) genes were amplified and sequenced. The obtained sequences were compared using BLASTn search algorithm in NCBI database and their phylogenetic analyses were performed based on single or multiple genomic regions. Accordingly, the isolates were identified as follow: Chaetomium anastomosans, C. ascotrichoides, C. subglobosum, Didymella pomorum, D. prolaticolla, and Paramicrosphaeropsis iranica. Based on literature, this is the first record of C. anastomosans, C. subglobosum and D. prolaticolla for the mycobiota of Iran. In addition, new hosts for C. anastomosans (P. atlantica, Q. brantii and Crataegus sp.), C. ascotrichoides (Acer sp.), C. subglobosum (Q. brantii) and P. iranica (A. scoparia and P. atlantica) are reported.

Keywords

Main Subjects

References

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Journal of Applied Research in Plant Protection
Volume 12, Issue 3
September 2023
Pages 239-256

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History

  • Receive Date: 05 July 2022
  • Revise Date: 18 December 2022
  • Accept Date: 21 October 2023

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