اثر دی اکسید کربن روی انتقال بذری ویروس موزاییک کاهو

Document Type : Short paper

Author

Plant Protection Department, Agriculture Faculty، University of Tabriz, Tabriz, Iran

Abstract

دی اکسید کربن (CO2)، عامل اثر گلخانه­ ای و افزایش گرمای کرۀ زمین، به عنوان مشکل اصلی جوامع بشری امروز است. این گاز بعنوان یکی از نیازمندی­ های اساسی رشد گیاهی و افزایش دهندۀ تولید بذر شناخته شده است. دربارۀ اثر مقادیر بالای CO2 (ECO2) روی انتقال بذری ویروس­ های گیاهی، تاکنون هیچ مطالعه ای صورت نگرفته است. در این مطالعه، بذور حاصل از کاهوی روییده تحت مقدار عادی (375 پی پی ام) و سطح بالای (750 پی پی ام) CO2 از بذور آلوده به ویروس یا مایه زنی شده با ویروس مورد بررسی گرفتند تا اثر ECO2 بر انتقال بذری تعیین گردد. دو نمونۀ بذری (هر کدام 25 بذر) از هر یک از گیاهان هر تیمار روی کاغذ فیلتری مرطوب در تشتک پتری جوانه زنی شدند و گیاهچه های  چهار روزه با DAS-ELISA با کانژوگۀ تهیه شده آزموده  شدند. نتایج نشان داد که انتقال در بذور حاصل از گیاهان روییده از بذور آلوده تحت ECO2  بالاتر(5/8 %) از انتقال بذری در گیاهان رشد کرده تحت سطح معمولی CO2 (8/6 %) بودند. همین­طور، انتقال در بذور حاصل از گیاهان آلوده با شیره گیاهی، تحت ECO2 بیشتر(17/4%) ازبذور گیاهانی  بود که  تحت شرایط معمولی CO2 رشد کرده بودند (4%). بعلاوه، میزان بذرزادی ویروس در گیاهان رشد کرده از بذور آلوده بیشتر (5/8 و 8/6 %) از مقدار آن در بذور حاصل از گیاهان آلوده شده با شیرۀ گیاهی بود (4 و 17/4%). 

Keywords


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