Effect of seed priming on germination indices and some biochemical mechanisms of cumin (Cuminum cyminum) against Fusarium wilt disea

Document Type : Research Paper

Authors

1Seed and Plant Certification and Registration Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Abstract

Abstract
Seed priming improves germination and growth components of seedlings under biotic and abiotic stress conditions. The aim of this study was to compare the effect of seed priming with chemical fungicides and isolates of Trichoderma harzianum on germination and vigor indices of native cumin population. Also, the activity and expression of phenylalanine ammonia lyase enzyme were evaluated as main markers of phenylpropanoid signal transduction pathway and accumulation of phenolic secondary metabolites. The results of effective inhibitory concentrations showed that the lowest values of minimum inhibitory and fungicidal concentrations of chemical fungicides against Fusarium oxysporum were related to Iprodione-Carbendazim (Rovral-TS®), Carbendazim (Bavistin®) and Carboxin thiram (Vitavax thiram®), respectively. The results also showed that T. harzianum isolates had fungistatic but not fungicide activity against F. oxysporum. The results of biochemical mechanisms showed that protein content and accumulation of phenolic and flavonoid compounds increased significantly in seedlings obtained from bioprimed seeds compared to healthy control. A significant relationship was observed between gene expression and phenylalanine ammonia lyase enzyme activity with phenolic and with flavonoid compounds, as well. Moreover, the results showed that isolates of T. harzianum and chemical fungicides caused activation of the antioxidant system and by increasing protein content and inducing the expression of phenylalanine ammonia lyase gene led to the production and accumulation of phenolic secondary metabolites in cumin. The findings of this research showed that seed priming, especially with T. harzianum isolates while improving seed quality and seedling health, increase the production and accumulation of phenolic secondary metabolites.
 

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References
 
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