Identification of Fusarium species associated with root rot symptoms of the rapeseed in Khuzestan province

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran. Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Abstract
Rapeseed is a host for a large number of pathogenic and non-pathogenic fungi, including Fusarium. During 2018–2020, 40 rapeseed samples showing yellowing, wilting and root rot were collected from different areas in Khuzestan province, which led to the isolation of 35 Fusarium isolates on potato-dextrose-agar (PDA). Ten isolates were selected based on the shape of the colonies and conidia and identified according to phylogenetic analysis and morphology. For molecular study, two regions including the internal transcribed spacer nuclear ribosomal DNA region (ITS) and translation elongation factor 1-α gene (TEF1) were amplified and sequenced. These sequences were analyzed by phylogenetic analysis using maximum likelihood and Bayesian inference algorithms. Accordingly, F. acuminatum, F. clavum, F. gracilipes, F. incarnatum and F. tanahbumbuense,and a representative species of the new lineage were identified. To our knowledge, this is the first report of the species Fusarium acuminatum, F. clavum, F. gracilipes, F. incarnatum and F. tanahbumbuense on rapeseed in Iran. Moreover, the species F. gracilipesis a new record for the Mycobiota of Iran.

Keywords


Ahmadi K, Ebadzadeh H, Hatami F, Abdeshah H, Kazemian A, 2019. Agricultural statistics of the crop year 2017-2018, Volume 1: crops. Ministry of Jihad Agriculture Publications, Tehran. 87 pp.
Ahmadpour SA, Mehrabi-Koushki M, Farokhinejad R, 2017. Neodidymelliopsis farokhinejadii, a new fungal species from dead branches of trees in Iran. Sydowia 69: 171–182.
Ainsworth GC, 1971. Ainsworth and Bisby’s Dictionary of the Fungi. 6th ed. Commonwealth Mycological Institute, Kew, Surrey, England. 663 pp.
Babaahmadi G, Mehrabi-Koushki M, Hayati J, 2018. Allophoma hayatii sp. nov., an undescribed pathogenic fungus causing dieback of Lantana camara in Iran. Mycological Progress 17 (3): 365–379.
Beneke ES, Rogers AL, 1996. Medical mycology and human mycoses. Star Publishing Company, Belmont. 239 pp.
Darriba D, Taboada GL, Doallo R, Posada D, 2012. jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772.
Edler D, Klein J, Antonelli A, Silvestro, 2019. raxmlGUI 2.0 beta: a graphical interface and toolkit for phylogenetic analyses using RAxMl. bioRxiv doi: 10.1101/800912.
Ellis JB, Everhart BM, 1895. New species of fungi from various localities. Proceedings of the Academy of Natural Sciences of Philadelphia 47: 413–441.
Elmer WH, 1996. Fusarium fruit rot of pumpkin in Connecticut. Plant Disease 80: 131–135.
Ershad D, 2009. Fungi of Iran, 3rd edn. Iranian research institute of plant protection, Tehran.
Farr DF, Rossman AY, 2020. Fungal databases, U.S. National Fungus Collections, ARS, USDA. Retrieved November 12, 2020, from https://nt.ars-grin.gov/fungaldatabases/
Gaetan S, 2005. Occurrence of Fusarium wilts on canola caused by Fusarium oxysporum f. sp. conglutinans in Argentina. Plant Disease 89: 432.
Hall TA, 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Heidari K, Farokhinejad R, Mehrabi-Koushki M, 2018a. Occurrence of purslane leaf spot caused by Dichotomophthora lutea in Iran. Australasian Plant Disease Notes 13 (1): 1–4.
Heidari K, Farokhinejad R, Mehrabi-Koushki M, 2018b. Detection and molecular identification of Colletotricum species causing anthracnose disease of some plants in Khuzestan province (Iran). Rostaniha 19(1): 19-32 (in Persian with English abstract).
Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Gvozdanović-Varga J, et al., 2017. First report of Fusarium acuminatum causing garlic bulb rot in Serbia. Plant Disease 101 (6): 1047–1048.
Irani H, Ravanlou A, 2006. Etiology and distribution of fungal crown and root rot of wheat in west Azarbaidjan Province. Journal of Agricultural Science (University of Tabriz) 16 (2): 45–56 (in Persian with English abstract).
Khezrinejad N, Rezae Danesh Y, 2017. Identification of Fusarium species associated with wheat, barley and corn in West Azarbaijan Province, Iran. Agroecology Journal 12 (4): 47– 60 (in Persian with English abstract).
Khojehpour MR, 2006. Industrial plants. Academic Press. 564 pp.   
Lange R, Mclaren DA, 2000. New Fusarium wilt of canola in Alberta. 1th Canadian Phytopathology Society AGM 3: 72–83.
Larki R, Mehrabi-Koushki M, Farokhinejad R, 2019. Ectophoma iranica sp. nov. and new hosts and records of Allophoma spp. in Iran. Journal of Phytopathology 167: 538–545.
Larki Z, Farokhinejad R, 2015. Identification of the Fusarium species associated with canola crown and root rot in Khuzestan Province. Journal of Microbial World 8 (2): 168-172 (in Persian with English abstract).
Lazreg F, Belabid L, Sanchez J, Gallego E, Garrido-Cardenas J A, et al., 2013. First report of Fusarium acuminatum causing damping-off disease on aleppo pine in Algeria. Plant Disease 97 (4): 557.
Li M, Murray GM, Ash GJ, 2007. New root diseases of canola in Australia. Australasian Plant Disease 2: 93-94.
Maryani N, Sandoval-Denis M, Lombard L, Crous PW, Kema GHJ, 2019. New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia. Persoonia 43: 48–69.
Mehrabi-Koushki M, Bavarsad M, Farrokhinejad R, Jamshid M, Alimohammadi A, 2016. The comparison of ITS-rDNA and tef1α genomic regions for phylogenetic study of some Trichoderma species. Iranian Journal of Plant Protection Science 47 (1): 61–70.
Naseripour T, Farokhinejad R, Sharif nabi B, 2008. Investigation of morphological, pathogenical and molecular aspects of Alternaria isolated from canola in Khuzestan and Kermanshah province. MSc Thesis, Plant Protection, Shahid Chamran University, Ahvaz.
Nasrisfahani M, Sanieirad A, Nasrollahi N, 2009. Identification of some pathogenic fungal agents of crown and roots of rapeseed in Isfahan. Plant Protection Journal 1 (2): 112-227 (in Persian with English abstract).
Neik TX, Martin J, Barbetti MJ, Batley J,2017. Current status and challenges in identifying disease resistance genes in Brassica napus. Frontiers in Plant Science 8: 30.
Nouran SL, Minasian V, Safarei N, 2008. Identification, pathogenicity and distribiution of Alternaria spp. of canola in Iran. Iranian Journal of Plant Pathology 44 (2): 137–154 (in Persian with English abstract).
O’Donnell K, Humber RA, Geiser DM, Kang S, Park B, et al., 2012. Phylogenetic diversity of insecticolous fusaria inferred frommultilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. Mycologia 104: 427–445.
O’Donnell K, Sutton DA, Rinaldi MG, Gueidan C, Crous PW, et al., 2009. Novel multilocus sequence typing scheme reveals high genetic diversity of human pathogenic members of the Fusarium incarnatum-F. equiseti and F. chlamydosporumspecies complexes within the United States. Journal of Clinical Microbiology 47: 3851–3861.
O’Donnell K, Sutton DA, Rinaldi MG, Sarver BAJ, Balajee SA, et al., 2010. Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. Journal of Clinical Microbiology 48: 3708–3718.
Raeder U, Broda P, 1985. Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1: 17–20.
Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, et al., 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542.
Santos ACS, Trindade JVC, Lima CS, Barbosa RN, Costa AF, et al., 2019. Morphology, phylogeny, and sexual stage of Fusarium caatingaense and Fusarium pernambucanum, new species of the Fusarium incarnatum-equiseti species complex associated with insects in Brazil. Mycologia 111(2): 244–259.
Sharifi K, Zare R, Zamanizadeh HR, Mirabolfathy M, Rezaee S, 2016. Identification of fusarium species associated with fusarium head blight of wheat in the north of Iran and phylogenetic analysis of the dominant species. Rostaniha 17(2): 173–187.
Shoukouhi E, Bissett J, 2008. Preferred primers for sequencing the 50 end of the translation elongation factor 1-alpha gene (EF1-a1) and subunit 2 of the RNA polymerase B gene (RPB2). ISTH available from: http://www.isth.info/methods.
Torbati M, Arzanlou M, Sandoval-Denis M, Crous PW, 2019. Multigene phylogeny reveals new fungicolous species in the Fusarium tricinctum species complex and novel hosts in the genus Fusarium from Iran. Mycological Progress 18: 119–133.
Villani A, Moretti A, Saeger S, Han Z, Mavungu JD, et al., 2016. A polyphasic approach for characterization of a collection of cereal isolates of the Fusarium incarnatum-equiseti species complex. International Journal of Food Microbiology 234: 24–35.
Wang MM, Chen Q, Diao YZ, Duan WJ, Cai L, 2019. Fusarium incarnatum-equiseti complex from China. Persoonia 43:70–89.
White TJ, Bruns T, Lee S, Taylor J, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ and White TJ. (eds) PCR protocols: a guide to methods and applications. Academic Press, New York. pp 315–322.
Xia JW, Sandoval-Denis M, Crous PW, Zhang XG, Lombard L, 2019. Numbers to names – restyling the Fusarium incarnatum-equiseti species complex. Persoonia 43: 186 – 221.