Detection of Gene Mutations Associated with Resistance to Benzimidazoles and Strobilurins in Botrytis cinerea Isolates from Grape in West Azarbaijan Province

Document Type : Research Paper

Authors

1 Detection of Gene Mutations Associated with Resistance to Benzimidazoles and Strobilurins in Botrytis cinerea Isolates from Grape in West Azarbaijan Province

2 Assisstant Professor, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran.

3 Assisstant Professor, Department of Plant Pathology, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran.

Abstract

Abstract
Chemical control using fungicides is considered as the most important method to control Botrytis cinerea in different crops. However, effective management of this pathogen requires information on presence and frequency of fungicide-resistant strains and levels of resistance as well as mutations associated with the resistance. In this research, sensitivity of 103 B. cinerea isolates collected from vineyards in different regions of West Azarbaijan province was assessed to carbendazim (60 WP) and azoxystrobin (25 SC). Among the studied isolates, 39 (38%) and 35 (34%) isolates were highly resistant to carbendazim and azoxystrobin, respectively. Partial sequencing of the beta tubulin gene indicated that in carbendazim-resistant strains GAG had changed to GCG which led to the substitution of glutamic acid by alanine at the codon position 198 and subsequently appearance of Ben R1 phenotype. Analysis of the partial cyt b gene sequence revealed that in azoxystrobin-resistant strains GGT had changed to GCT (point mutation) leading to substitution of glycine by alanine at the codon position 143. Further, one of the azoxystrobin-sensitive isolates had Bcbi-143/144 intron immediately after codon 143 in cyt b. The results indicated that application of benzimidazole and strobilurin fungicides groups are not recommended for B. cinerea management in the vineyards of the region. However, given that benzimidazole-resistant strains had Ben R1 phenotype, benzimidazole fungicides can be used with diethofencarb or zoxamide as a mixture partner to effectively control both sensitive and resistant strains.
 

Keywords

References

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Journal of Applied Research in Plant Protection
Volume 8, Issue 1
June 2019
Pages 57-70

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History

  • Receive Date: 22 June 2019
  • Accept Date: 22 June 2019

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