Identification of Vegetative Compatibility Groups and Clonal Lineages of Fusarium solani, the Causal Agent of Bean Root Rot in Zanjan, Iran

Document Type : Research Paper

Authors

1 MSc Student of Plant Pathology, Dept of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

2 Assistant Professor, Dept of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

Abstract

Vegetative compatibility groups of 42 isolates of Fusarium solani, the causal agent of root rot, isolated from different bean cultivation regions of Zanjan province, Iran were determined using nit mutants. The phenotype of nit mutants was detected according to their developmental characters in minimal medium (MM) containing different nitrogen sources and nitM was found as the most abundant nit mutant. Complementary experiments were conducted among the self-compatible isolates and accordingly the isolates were grouped in 16 VCGs, with four single-isolate and 12 multi-isolate VCGs. There were bridging isolates, the isolates compatible with two or more VCGs, among multi-isolate groups. There was no correlation between vegetative compatibility groups and geographic origin of the isolates. To identify clonal lineages, one primer (OPA-13) among eight RAPD primers, amplified appropriate and polymorphic DNA patterns. Polymerase Chain Reaction was successful for 26 out of 42 isolates. The results of molecular data analysis showed high diversity among the isolates and according to these results 13 clonal lineages were identified among 26 isolates (similarity coefficient: 75%). Each clonal lineage included only one single or multi-member haplotype. There was no correlation between haplotypes and VCGs.

Keywords

References

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Journal of Applied Research in Plant Protection
Volume 2, Issue 1 - Serial Number 1
January 2014
Pages 67-83

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History

  • Receive Date: 17 June 2013
  • Revise Date: 21 January 2014
  • Accept Date: 06 November 2013

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