Morphological and Molecular Characterization of Fungal Species Associated with Olive Fruit Rot Disease in Zanjan Province, Iran

Document Type : Research Paper

Authors

1 Former MSc Student, Dept of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Associate Professor and Professor, Dept of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

3 Assistant Professor, Agricultural and Natural Resources Research Center of Zanjan Province, Iran

4 Professor, Dept of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Abstract

Fruit rots are among the most important diseases of olive in Zanjan province, Iran. Despite the incidence of the disease in the region, the fungal species involved in occurrence of the disease largely remain unknown. The aim of present study is to characterize fungal species associated with olive fruit rot in Zanjan province based on morphological and molecular data. For this purpose, olive fruit samples showing fruit rot symptoms were collected from olive orchards in Zanjan province, during the September and October 2011. Pure cultures were established using a single spore technique. Fungal isolates were identified using morphological characteristics as well as sequence data of ITS-rDNA region. The results revealed Alternaria alternata species complex as the dominant species amongst the fungal groups involved in olive fruit rot in this region with a frequency of 37 percent. The identity of Alternaria isolates were further analysed based on sequence data from the rpb2 and gpd genes. The phylogeny inferred using sequence data from rpb2 gene culstred olive isolates in A. alternata species complex clade. The other fungal species identified in this study include Acremonium implicatum, Arthrinium phaeospermum, Aspergillus ochraceus, Aureobasidium pullulans, Botrytis cinerea, Epicoccum nigrum, Fusarium sp., F. nygamai, F. proliferatum, Penicillium expansum, Trichoderma harzianum and Truncatella angustata. Most of the species represent new records for Iran. With the identification of fungal species associated with olive fruit rot, it will be possible to practice suitable management strategies for this disease.

Keywords

References

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Journal of Applied Research in Plant Protection
Volume 2, Issue 1 - Serial Number 1
January 2014
Pages 53-66

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History

  • Receive Date: 17 March 2013
  • Revise Date: 21 January 2014
  • Accept Date: 11 October 2013

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