Different biological behavior in the interaction of Erwinia persicina and Xanthomonas translucens, two wheat seed-borne bacteria, in vitro and in vivo

Document Type : Research Paper

Authors

1 Department of Plant Protection, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Seed and Plant Certification and Registration Institute. Agricultural Research, Education and Extension Organization (AREEO). Tehran, Iran.

3 Department of Plant Protection, Islamic Azad University, Science and Research Branch, Tehran, Iran.

4 Seed and Plant Certification and Registration Institute. Agricultural Research, Education and Extension Organization (AREEO).

Abstract

Erwinia persicina (Ep) is a plant pathogenic bacterium with a wide range of host's, spread through infected seeds. Seeds carry a variety of surface microbes with complex interactions with each other and the host plant. The bacterium Ep was isolated during surveys from wheat fields infected with Xanthomonas translucens (Xt), the cause of bacterial leaf streak disease. From the ten samples of Mihan cultivar collected from different regions of Iran, just one Ep isolate was isolated from Markazi province and two isolates from Lorestan province. The isolated bacteria were identified based on some key biochemical traits and 16Sr RNA sequence analysis. The pathogenic or antagonistic activities of these three isolates were different. All isolates (Ais155, Ais238 and Ais255) produced an effective inhibitory zone against Xt under in vitro conditions. At the early stage after sowing the Ep treated seeds in artificially contaminated soil with Xt, the typical disease symptoms appeared weak with delay but it seems that the production of biofilm and enzyme activities of E. persicina (Ais155 isolate) and particularly high cellulase activity (in Ais238 isolate) cause more severe incidence of bacterial leaf streak disease (18.3 to 39.6%) in comparison with untreated seeds in infected soil. Indirect effect of seed treatment on disease severity were assayed through inoculum spraying plantlets that obtained from treated seeds. The Ais255 isolate indirectly increased plant relative resistance to Xt. It was concluded that Ep can reduce the severity of the disease via activating the plant's defense system. Further research is needed to prove the different dimensions of this effect.

Keywords

References

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Journal of Applied Research in Plant Protection
Volume 13, Issue 2
July 2024
Pages 193-203

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History

  • Receive Date: 04 November 2023
  • Revise Date: 21 January 2024
  • Accept Date: 23 January 2024

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