Effects of nanoencapsulated formulation of two different medicinal plants essential oil using tragacanth gum in control of post-harvest penicillium decay of tomato fruit

Document Type : Research Paper

Authors

1 Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.

2 Plant Protection Research Department, West Azarbaijan Agricultural and Natural Resources Research Center, AREEO, Urmia, Iran

Abstract

The use of medicinal plants’ essential oils, as an alternative to chemical fungicides, is limited due to their low solubility in water, poor chemical stability, and their volatile nature. To solve this problem, encapsulation of essential oil on a nanometer scale and combining it with coating polymers has been suggested recently. In this study, nanoemulsions of Satureja khuzistanica (Khuzestani savory) essential oil (containing 87.4% carvacrol) and Mentha piperita (peppermint) essential oil (containing 33.6% menthone and 35.3% menthol) were prepared with an average particle size of 295 and 186 nm, respectively. After confirming their effectiveness against the fungus Penicillium expansum, they mixed with tragacanth coating polysaccharide (0.5% and 1%) to inhibit the penicillium decay of tomato fruit as well as improve fruit physicochemical indicators during storage at 10°C. Fruit treatments by nanoemulsion of essential oil (NEO) of S. khuzistanica 0.1% in combination with tragacanth 0.5% and 1% showed the greatest effect to inhibit the penicillium decay. The fruits treated with the NEO of peppermint was placed in the next statically group. Furthermore, the effect of the superior treatments on some physicochemical indicators of fruit showed a significant positive effect of them in terms of the fruit firmness and its total soluble solids (TSS). In addition, the total phenolic content of the treated fruits was significantly higher than non-treated healthy fruits. However, it seems that nanoencapsulation of antifungal and antioxidant essential oils compounds and combining them with the tragacanth, caused to increases the shelf life of the fruit during storage.

Keywords

References

References
Adams RP, 2007. Identification of essential oils components by Gas Chromatography / Quadrupole Mass Spectrometry. 4th edition, Allured Publishing Allured Publishing Corporation, Carol Stream, Illinois. 804 pp.
Al-Mariri A, Safi M, 2014. In vitro antibacterial activity of several plant extracts and oils against some Gram-negative bacteria. Iran Journal of Medical Science 39: 36–43.
Ardalan F, 2014. Formulation of nano-emulsions from essential oil of Cinnamomum zeylanicum in treatment of Helicobacter pylori infection. Master of Science thesis, Medicinal Plants and Drug Research Institute, Shahid Beheshti University. Pp.72. (In Persian with English abstract).
 Atrash A, Ramezanian A, Rahemi M, 2018. Antifungal Effects of Savory Essential Oil, Gum Arabic, and Hot Water in Mexican Lime Fruits. Hortscience 53(4): 524–530.
Aumelas A, Serrero A, Durand A, Dellacherie E, Leonard M, 2007. Nanoparticles of hydrophobically modified dextrans as potential drug carrier systems. Colloids Surf B Biointerfaces 59(1): 74–80.
Beykia M, Zhaveha S, Khalilib S T, Rahmani-Cheratic T, Abollahic A, 2014. Encapsulation of Menha piperita essential oils in chitosan–cinnamic acid nanogel with enhanced antimicrobial activity against Aspergillus flavus. Industrial Crops & Products 54:  310–319.
British Pharmacopoeia, 2015. Vol. IV. Medicines and Healthcare Products Regulatory Agency (MHRA), London. 762 pp.
Chang Y, McLandsborough L, McClements D, 2012. Physical properties and antimicrobial efficacy of thyme oil nanoemulsions: Influence of ripening inhibitors. Journal of Agricultural and Food Chemistry 60 (48): 12056–12063.
  Chang Y, McLandsborough L, McClements DJ, 2013. Physicochemical properties and antimicrobial efficacy of carvacrol nanoemulsions formed by spontaneous emulsification. Journal of Agricultural & Food Chemistry 61 (37): 8906–8913.
Enayati S, Davari M, Habibi-Yangjeh A, Ebadollahi A, 2023. Mycelial inhibitory effects of five essential oils and two antagonistic Trichoderma species against some plant pathogenic Fusarium species. Journal of Applied Research in Plant Protection 11 (4): 29–42.
FAO. 2021. FAOSTAT: Crops. In: FAO. Rome. fao.org/faostat/en/#data/QC
Farzaneh M, Kiani H, Sharifi R, Reisi M, Hadian J, 2015. Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest Biology & Technology 109: 145–151.
Fernandez-Panchón MS, Villano D, Troncoso A, M García-Parrilla MC, 2008. Antioxidant activity of phenolic compounds: from in vitro results to in vivo evidence. Critical Reviews in Food Science 48: 649–671.
Ghaderi L, 2014. Formulation of nanoemulsions from essential oil of Thymus daenensis in treatment of sinusitis. Master of Science thesis, Medicinal Plants and Drug Research Institute, Shahid Beheshti University. Pp.96. (In Persian with English abstract)
Gyawali R, Ibrahim SA, 2014. Natural products as antimicrobial agents. Journal of Food Control 46: 412–429.
Huang R, Li GQ, Zhang J, 2011. Control of postharvest Botrytis fruit rot of strawberry by volatile organic compounds of Candida intermedia.  Phytopathology 101 (7): 859–869.
Kader AA, 2004. Increasing food availability by reducing postharvest losses of fresh produce. V International Postharvest Symposium 682: 2169–2176.
Kanetis L, Förster H, Adaskaveg, J E, 2007. Comparative efficacy of the new postharvest fungicides azoxystrobin, fludioxonil, and pyrimethanil for managing citrus green mold. Plant Disease. 91:1502–1511.
Matan N, Rimkeeree H, Mawson A, Chompreeda P, Haruthaithanasan V, Parker M, 2006. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. International Journal of Food Microbiology 107(2): 180–185.
Mazumdar BC, 2003. Methods on physico-chemical analysis of fruit, Daya Publishing House, Delhi. 187pp.
 McClements DJ, 2016. Food Emulsions: Principles, Practices, and Techniques. 3rd ed. New York: CRC Press Publ. 1–26.
Mckay DL, Blumberg JB, 2006. A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.)”. Phytotherapy Research Protection. 20(7): 519–530.
Moghimi R, Ghaderi L, Rafati H, Aliahmadi A, McClements DJ, 2016. Superior antibacterial activity of nanoemulsion of Thymus daenensis essential oil against E. coli. Journal of Agricalture & Food Chemistry 194: 410–415.
 Omidbeygi M, Barzegar M, Hamidi Z, Naghdibadi H, 2007. Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control  18 (12): 1518–1523.
Onyuksel H, Krishnadas A, Rubinstein I, 2003. Sterically stabilized phospholipid mixed micelles: In vitro evaluation as a novel carrier for water-insoluble drugs. Pharmaceutical Research 20(2): 297–302.
 Pandey S, Ashutosh VP, Tripathi A, Kumari M, Narayan S, 2020. Early blight disease management by herbal nanoemulsion in Solanum lycopersicum with bio-protective manner. Industrial Crops Products  150: 112– 121.
 Pourhossein-Alamdary M, 2012. Design formulation of Satureja khuzistanica essential oil nano-emulsions in treatment of sinusitis. MSc thesis, Medicinal Plants and Drug Research Institute, Shahid Beheshti University, Tehran, Pp.116. (in Persian with English abstract).
Reddy M, Angers VB, Gosselin PA, Arul J, 1998. Characterization and use of essential oil from Thymus vulgaris against Botrytis cinerea and Rhizopus stolonifer in strawberry fruits. Photochemistry 47 (8): 1515– 1520.
Sanjarian M, Rakhshandehroo F, Rezaee S, 2021. The effect of persian lilac and fig plant crude aquatic and ethanolic extracts on disease caused by Cucumber mosaic virus in cucumber (Cucumis sativus) plants under greenhouse condition. Journal of Applied Research in Plant Protection 10 (2): 47–61.
Shah B, Davidson, PM, Zhong QJA, 2012. Nano-dispersing thymol for enhanced dispersibility and antimicrobial effectiveness against Escherichia coli O157: H7 and Listeria monocytogenes in model food systems. Applied Environmental Microbiology 78(23): 8448–53.
Shah B, Davidson PM, Zhong QJ, 2013. Antimicrobial activity of nanodispersed thymol in tryptic soy broth. Journal of Food Protection.76 (3): 440–447.
Sharifi-tehrani A, Farzaneh M, 2018. Fungicides (history, mode of action, resistance and application in plant protection), University of Tehran, Tehran. (in Persian). 443pp.
Slinkard K, Singleton VL, 1977. Total phenol analyses:
Aautomation and comparison with manual methods. American. Journal of Enology & Viticulture 28: 49–55.
Sokovic M, van Griensven, LJLD, 2006. Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus. European Journal of Plant Pathology 116: 211–224.
Solans C, Izquierdo P, Nolla J, Azemar N, Garcia-Celma M J, 2005. Nano-Emulsions. Current Opinion in Colloid and Interface Science 10(3–4): 102–110.
Sozer N, Kokini, JL, 2009. Nanotechnology and its applications in the food sector. Journal of Colloid &   Interface Science 27(2): 82–89.
Stević T, Berić T, Katarina Šavikin K, Soković M, Gođevac D, 2014. Antifungal activity of selected essential oils against fungi isolated from medicinal plant. Industrial Crops & Products 55: 116–122.
Tajkarimi M, Ibrahim SA, Cliver DJ, 2010. Antimicrobial herb and spice compounds in food. Journal of Food Control 21(9): 1199–1218.
Tripath P, Dubey NK, Shukla AK, 2008. Use of some essential oils as post-harvest botanical fungicides in the management of grey mold of grapes caused by Botrytis cinerea. World Journal of Microbiology & Biotechnology 21(1): 1 39–46.
Valková V, úranová H Dˇ, Vukovic N L, Vukic M, Kluz M, Kaˇcániová M, 2022. Assessment of chemical composition and anti-penicillium activity of vapours of essential oils from Abies alba and Two Melaleuca Species in Food Model Systems. Molecules 27: 3101.
Wills R, Golding J, 2016. Postharvest: an introduction to the physiology and handling of fruits and vegetables. 6th edition, CABI, Boston, USA. 320 pp.
Xue Y, Liu Q, He G, Xu K, Jiang L, 2013. Excellent electrical conductivity of the exfoliated and fluorinated hexagonal boron nitride nanosheets. Nanoscale Research Letter 8: 49.
Znidarcic, D., Ban, D., Oplanic, M., Karic, L. Pozrl, T, 2010. Influence of postharvest temperatures on physicochemical quality of tomatoes (Lycopersicon esculentum Mill.). Journal of Food Agriculture & Environmental 8(1): 21–25.
Journal of Applied Research in Plant Protection
Volume 12, Issue 4
December 2023
Pages 413-424

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History

  • Receive Date: 14 February 2023
  • Revise Date: 22 June 2023
  • Accept Date: 16 July 2023

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