Specific identification of root-knot nematode Meloidogyne incognita common in kiwifruit orchards of Guilan and Mazandaran provinces using sec-1 gene sequence

Document Type : Research Paper

Authors

1 Plant Protection Department, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

2 Horticultural Science Research Institute, Citrus and Subtropical Fruits Research Center, Agricultural Research Education and Extension Organization (AREEO), Ramsar, Iran,

3 Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.

Abstract

In recent years, the increasing prevalence of root knot nematodes has caused serious damage to kiwifruit cultivation in northern Iran. Due to the prevalence of several species of this genus in the country, accurate identification of species of this nematode in the kiwifruit industry is important. In this study, we attempted to identify the root knot nematode species by polymerase chain reaction, using specific primers of the gene encoding one of the pharyngeal glands secretory proteins (sec-1) to confirm the morphological identifications. After sampling and isolation of the nematodes from kiwifruit orchards in Guilan and Mazandaran provinces, initial identification was performed using morphological characteristics. The Inc-k14 primer pair was used to amplify the SCAR region and the SEC-1 primer pair was used to amplify the sec-1 gene for specific identification of Meloidogyne incognita from the other species. In all samples, a 399 bp was amplified by Inc-K14 primers and a 502 bp fragment was amplified by SEC-1 primers, which are expected as specific bands for M. incognita. The PCR test using Rjav/Fjav, Rar/Far, and Rh/Fh primers, which are specific primers of M. javanica, M. arenaria and M. hapla, respectively, did not lead to band amplification in this study. According to the results, SEC-1 primer is suitable for rapid and accurate identification of M. incognita in infested kiwifruit orchards (in present study) and will not have non-specific band amplification problems using Inc-K14 primers that amplify the SCAR region.

Keywords

References

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Journal of Applied Research in Plant Protection
Volume 11, Issue 4
December 2022
Pages 63-72

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History

  • Receive Date: 15 January 2022
  • Revise Date: 05 February 2022
  • Accept Date: 02 April 2022

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