Effect of endophytic Beauveria bassiana on growth traits of five rapeseed varieties Brassica napus

Document Type : Research Paper

Authors

1 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;

2 Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;

3 Department of Entomology, Texas A&M University, College Station, Texas, USA.

Abstract

Today, development of rapeseed Brassica napus cultivation has been considered due to the need to produce edible oil. Using chemical pesticides and fertilizers for the product increase has had detrimental effects on the environment and food safety. Applying probiotic agents could increase rapeseed improvement and growth factors besides promoting crop resistance to pests and diseases in sustainable agriculture. Thus, the endophytic role of Beauveria bassiana as the most well-known insect pathogenic fungus was investigated in five rapeseed varieties as Okapi, Nepton, Nafis, Nima, and Atura in terms of establishment and improvement of their growth indices. The seeds of these cultivars were treated by immersion in fungal suspensions of 106 and 107 conidia per­ ml and by coating with fungus conidial powder. Sampling was performed to determine the presence of fungus in the plant and to measure growth factors, until 21 days after inoculation. To re-isolate the fungus from the plant tissues, the tissues were plated on SDAY and evaluated after 10 days. The results showed that the fungus was well established as an endophyte in all varieties. Analysis of data on growth variables showed that there was a significant difference between treated and control plants for each variety. Nepton demonstrated a better response. Since this cultivar is one of the winter varieties that is commonly cultivated in Iran, seed treatment by the fungus could be used operationally in our country. This will improve the growth traits of the strategic plant, rapeseed, and increase the yield indices, ultimately.

Keywords

References

Adesemoye A O, Kloepper J W, 2009. Plant–microbes interactions in enhanced fertilizer-use efficiency. Applied Microbiology and Biotechnology 85 (1): 1–12.
Azimi S, Shahin S, Alizadeh A, 2021. Evaluation of bean plant treatment with Trichoderma harzianum TR6 on the biology of bean aphid Aphis fabae. Journal of Applied Research in Plant Protection 10 (1):1–6 (In Persian with English abstract).
Batta Y A, 2013. Efficacy of endophytic and applied Metarhizium anisopliae (Metch.) Sorokin (Ascomycota: Hypocreales) against larvae of Plutella xylostella L. (Yponomeutidae: Lepidoptera) infesting Brassica napus plants. Crop Protection 44: 128–134.
Begum S R, Tamilselvi K, 2016. Endophytes are plant helpers: an overview. International Journal of Current Microbiology and Applied Sciences 5: 424–436.
Chitnis V R, Suryanarayanan T S, Nataraja K N, Prasad S R, Oelmüller R, Shaanker R U, 2020. Fungal endophyte-mediated crop improvement: the way ahead. Frontiers in Plant Science 11: 1588.
Dara S K, 2019. Non-Entomopathogenic Roles of Entomopathogenic Fungi in Promoting Plant Health and Growth. Insects 10(9): 277.
Greenfield M, Gómez-Jiménez M I, Ortiz V Vega, F E Kramer M, Parsa S, 2016. Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. Biological Control 95: 40–48.
Hasnain M., Chen J, Ahmed N, Memon S, Wang L, Wang Y, Wang P, 2020. The Effects of Fertilizer Type and Application Time on Soil Properties, Plant Traits, Yield and Quality of Tomato. Sustainability 12 (21): 9065.
Heviefo GA, Nyamador SW, Datinon BD, Glitho IA, Tamò M, 2020. Comparative efficacy of endophytic versus foliar application of the entomopathogenic fungus Beauveria bassiana against the crucifer diamondback moth larvae for sustainable cabbage protection. International Journal of Biological and Chemical Sciences 14 (4): 1448–1458.
Hodkinson TR, Doohan FM, Saunders MJ, Murphy BR, 2019. Endophytes for a growing world. Cambridge University Press. 445 pp.
Iacovidou E, Gerassimidou S, 2018. Sustainable Packaging and the Circular Economy: An EU Perspective. Reference Module in Food Science. In: Smithers G, Trinetta V, Knoerzer K (eds). Elsevier, Amsterdam. Pp. 1–16.
Itelima J, Bang W, Onyimba I, Sila M, Egbere O, 2018. Bio-fertilizers as key player in enhancing soil fertility and crop productivity: A review. Direct Research Journal of Agriculture and Food Science 6 (3): 73–83.
Jaber LR, Enkerli J, 2016. Effect of seed treatment duration on growth and colonization of Vicia faba by endophytic Beauveria bassiana and Metarhizium brunneum. Biological Control 103: 187–195.
Jaber LR, Ownley BH, 2018. Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens? Biological Control 116: 36–45.
Johnson DM, White RL, Pereira RM, Geden CJ, 2020. Beauveria bassiana culturing and harvesting for bioassays with house flies. Journal of Insect Science 20 (6): 14.
Lohse R, Jakobs-Schönwandt D, Vidal S, Patel AV, 2015. Evaluation of new fermentation and formulation strategies for a high endophytic establishment of Beauveria bassiana in oilseed rape plants. Biological Control 88: 26–36.
Lopez DC, Sword GA, 2015. The endophytic fungal entomopathogens Beauveria bassiana and Purpureocillium lilacinum enhance the growth of cultivated cotton (Gossypium hirsutum) and negatively affect survival of the cotton bollworm (Helicoverpa zea). Biological Control 89: 53–60.
McKinnon AC, Saari S, Moran-Diez ME, Meyling NV, Raad M, Glare TR, 2017. Beauveria bassiana as an endophyte: a critical review on associated methodology and biocontrol potential. BioControl 62 (1): 1–17.
Murphy BR, Doohan FM, Hodkinson TR, 2017. A seed dressing combining fungal endophyte spores and fungicides improves seedling survival and early growth in barley and oat. Symbiosis 71 (1): 69–76.
Narmani A, Arzanlou M, Babaiahari A, Masteri Farahani H, 2019. Biological control of wheat fusarium head blight using antagonistic strains of commercial and local Trichoderma, isolated from wheat plant rhizosphere. Journal of Applied Research in Plant Protection 8 (2):1–2 (In Persian with English abstract).
Sánchez-Rodríguez AR, Raya-Díaz S, Zamarreño ÁM, García-Mina JM, del Campillo MC, Quesada-Moraga E, 2018. An endophytic Beauveria bassiana strain increases spike production in bread and durum wheat plants and effectively controls cotton leafworm (Spodoptera littoralis) larvae. Biological Control 116: 90–102.
Seyed Talebi FS, Safavi SA, Talaei-Hassanloui R, Bandani AR, 2018. Study of virulence conidia germination types for some Beauveria bassiana isolaes. Biological Control of Pests and Diseases 7: 65–73.
Tall S, Meyling NV, 2018. Probiotics for plants? Growth promotion by the entomopathogenic fungus Beauveria bassiana depends on nutrient availability. Microbial Ecology 76 (4): 1002–1008.
Tian X, Li Z, Wang L, Wang Y, Li B, Duan M, Liu B, 2020. Effects of biochar combined with nitrogen fertilizer reduction on rapeseed yield and soil aggregate stability in upland of purple soils. International Journal of Environmental Research and Public Health 17 (1): 279.
Vega FE, 2018. The use of fungal entomopathogens as endophytes in biological control: a review. Mycologia 110 (1): 4–30.
Wei QY, Li YY, Xu C, Wu YX, Zhang YR, Liu H, 2020. Endophytic colonization by Beauveria bassiana increases the resistance of tomatoes against Bemisia tabaci. Arthropod-Plant Interactions 14 (3): 289–300.
Yazdandoust-Hamedani M, 2020. Introducing internal cultivation of rapeseed in cold and moderate climates of Iran. In: Ojaghi S, Pourfattah N (eds). Agricultural Education Press. 20 pp. (In Persian)
Ye L, Zhao X, Bao E, Li J, Zou Z, Cao K, 2020. Bio-organic fertilizer with reduced rates of chemical fertilization improves soil fertility and enhances tomato yield and quality. Scientific Reports 10 (1): 1–11.
Zareei Siahbidi A, Asgari A, 2020. Response of oilseed rape hybrids and open pollinated genotypes to seeding rate. Journal of Agricultural Science and Sustainable Production 30 (4): 95–109 (In Persian with English abstract).
Journal of Applied Research in Plant Protection
Volume 12, Issue 1
April 2023
Pages 87-94

Files

History

  • Receive Date: 03 June 2022
  • Revise Date: 24 July 2022
  • Accept Date: 25 July 2022

Share

How to cite

Statistics