Characterization of Pyrenophora, Bipolaris, Curvularia and Exserohilum species associated with leaf spot diseases on barley in East Azerbaijan province, using morphological and molecular data

Document Type : Research Paper

Authors

Department of Plant Protection, Faculty of Agriculture, University of Tabriz, 5166614766, Tabriz, Iran

Abstract

Abstract
Foliar diseases are considered among important barely diseases worldwide, causing economic crop losses in barely industry worldwide, including Iran. Present study was aimed to characterize Pyrenophora, Bipolaris, Curvularia and Exserohilum species associated with leaf spot diseases of barley in East Azerbaijan province, using consolidated species concept. Totally 462 fungal isolates were isolated and purified from barley plants representing leaf spot symptoms during 2016-2017. Based on morphological and sequence data of ITS-rDNA and Glyceraldehyde-3-Phosphate Dehydogenase gene (gpdh) regions. A considerable diversity was observed among the fungal species associated with leaf spot diseases on barley. In this study, Pyrenophora graminea, P. teres, Bipolaris sorokinana, B. salkadehensis, Curvularia inaequalis, C. spicifera, C. nicotiae and Exserohilum rostratum were identified. Pyrenophora graminea and P. teres were dominant species associated with brown leaf spot symptoms, with isolation frequencies of 68.4 and 25.54 percent, respectively. With the identification of fungal species associated with leaf spot diseases on barley, it will be possible to practice suitable management strategies for these diseases.

References

References
 
Abbasi M, Aliabadi F, 2009. The list of fungi reported in proceedings of 12th to 18th Iranian plant protection congress. Elm & Honar Publication 272 p.
Ahmadpour A, Donyadoost-Chelan M, Heidarian Z, Javan-Nikkhah M, 2011. New species of Bipolaris and Curvularia on Grass species in Iran. Rostaniaha 13: 69–82.
Ahmadpour A, Heidarian Z, Donyadoost-Chelan M, Javan-Nikkhah M, Tsukibosh T, 2012. A new species of Bipolaris from Iran. Mycotaxon 120: 301–307.
Al-Daoude A, Arabi MIE, Nabulsi I, MirAli N, 2012. Molecular phylogeny of Pyrenophora graminea as determined by RAPD and ISSR fingerprints. Journal of Plant Biology Research 1 (2): 25–35.
Aminnejad M, Babai-Ahari A, Javan-Nikkhah M, Hejazi MS, 2009. Molecular and Pathogenic Variation Within Iranian Pyrenophora graminea Population; More Polymorphism in IGS Region. American-Eurasian Journal of Agricultural and Environmental Science 6 (6): 697–704.
Arabi MIE, Jawhar M, 2004. Identification of Cochliobolus sativus (Spot Blotch) isolates expressing differential virulence on barley genotypes in Syria. Journal of Phytopathology 152: 461–464.
Ariyawansa HA, Kang JC, Alias SA, Chukeatirote E, Hyde KD, 2014. Pyrenophora. Mycosphere 5 (2): 351–362.
Asqari B, Zare B, Payghami E, 2005. Hyphomycetous fungal community of barley phylloplane in East Azarbaijan province with emphasis on new taxa for Iranian fungal flora. Rostaniha 5 (2): 171‒197.
Berbee ML, Pirseyedi M, Hubbard S, 1999. Cochliobolus phylogenetics and the origin of known highly virulent pathogens Inferred from ITS and glyceraldehydes-3-dehydrogenase gene sequence. Mycologia 91: 964–977.
Bovill J, Lehmensiek A, Sutherland MW, Platz GJ, Usher T, Franckowiak J, 2010. Mapping spot blotch resistance genes in four barley populations. Molecular breeding 26 (14): 653–666.
Duveiller E, Gilchrist L, 1994. Production constraints due to Bipolaris sorokiniana in wheat: Current situation and future prospects. In: Sounders D.A., Hettel G.P. (eds). Wheat in Heat-stressed Environments: Irrigated, Dry Areas and Rice-Wheat Systems 343–352.
Ellis MB, 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Kew, England Pp. 608.
Ellis MB, 1976. More dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew, England Pp. 507.
Ershad D, 2009. Fungi of Iran.3rd ed. Iranian Research Institute of Plant Protection.
Farr DF, Rossman AY, 2020. Fungal Databases, U.S. National Fungus Collections, ARS, USDA. Retrieved December 12, 2020. Available from: https://nt.ars-grin.gov/fungaldatabases/.
Gangwar OP, Bhardwaj SC, Singh GP, Prasad P, Kumar S, 2018. Barley disease and their management: An Indian perspective. Wheat and Barley Research 10(3): 138‒150.
Heidarian Z, Arzanlou M, Babaei-ahari A, Ahmadpour A, 2016. Phenotypic and molecular characterization of Exserohilum species from Iran. Nova Hedwigia 103: 327‒338.
Heidarian Z, 2017. Study on diversity of Cochliobolus and allied genera on graminicolous plants based on morphological and molecular methods in West Azerbaijan province. PhD thesis, Plant Pathology, Tabriz University, Iran.
Heidarian Z, Arzanlou M, Ahmadpour A, 2020. Molecular phylogeny and morphology differentiate several new records and novel hosts for Curvularia species in Iran. Nova Hedwigia 111: 151–171.
Hernández-Restrepo M; Madrid H; Tan YP; Cunha KC Da, Gené J; et al., 2018. Multi-locus phylogeny and taxonomy of Exserohilum. Persoonia 41: 71–108.
Iftikhar S, Asad S, Munir A, Sultan A, Ahmad I, 2009. Host of Bipolaris sorokiniana, the major pathogen of spot blotch of wheat in Pakistan. Pakistan Journal of Botany 41(3): 1433‒1436.
Jalli M, Laitinen P, Latvala S, 2011. The emergence of cereal fungal diseases and the incidence of leaf spot diseases in Finland. Agricultural and food science 20: 62–73.
Katoh K, Standley DM, 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772– 780.
Kumar J, Schafer P, Huckelhoven R, Langen G, Baltruschat H et al., 2002. Bipolaris sorokiniana, a cereal pathogen of global concern: cytological and molecular approaches towards better control. Molecular Plant Pathology 3: 185–195.
Kumar S, Stecher G, Tamura K, 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology and Evolution 33 (7): 1870–1874.
Lammari HI, Rehfus A, Stammler G, Fellahi ZE, Benbelkacem A, et al., 2020. Occurrence and frequency of spot form and net form of net blotch disease of barley in Algeria. Journal of Plant Diseases and Protection 127 (1): 35–42.
Liu D, Coloe S, Baird R, Pederson J, 2000. Rapid mini-preparation of fungal DNA for PCR. Journal of Clinical Microbiology 38 (1): 471.
Liu Z, Ellwood SR, Oliver RP, Friesen TL, 2011. Pyrenophora teres: Profile of an increasingly damaging barley pathogen. Molecular Plant Pathology 12 (1): 1–19.
Manamgoda DS, Cai L, McKenzie EHC, Crous PW, 2012. A phylogenetic and taxonomic re-evaluation of the Bipolaris, Cochliobolus, Curvularia Complex. Fungal Diversity 56: 131–144.
Manamgoda DS, Rossman AY, Castlebury LA, Chukeatirote E, Hyde KD, 2015. A taxonomic and phylogenetic re-appraisal of the genus Curvularia (Pleosporaceae): human and plant pathogens. Phytotaxa 212: 175–198.
Marin-Felix Y, Hernández-Restrepo M, Iturrieta-Gonzalez I, Gaecia D, Gene JZ, et al., 2019. Genera of Phytopathogenic Fungi: GOPHY 3. Studies in Mycology 94: 1–124.
Marin-Felix Y, Hernández-Restrepo M, Crous PW, 2020. Multi-locus phylogeny of the genus Curvularia and description of ten new species. Mycological Progress 19:559–588.
McDonald BA, Zhan J, Burdon JJ, 1999. Genetic structure of Rhynchosporium secalis in Australia. Phytopathology 89: 639–645.
Nylander JAA, 2004. MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Ronquist F, Huelsenbeck JP, 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574.
Si E, Meng Y, Ma X, Li B, Wang J, et al., 2019. Development and characterization of microsatellite markers based on whole-genome sequences and pathogenicity differentiation of Pyrenophora graminea, the causative agent of barley leaf stripe. European Journal of Plant Pathology 154: 227–241.
Sivanesan A, 1987. Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. Mycological Papers 158: 1–261.
Sun G, Oide S, Tanaka E, Shimizu K, Tanaka C, et al., 2003. Species separation in Curvularia “geniculata” group inferred from Brn1 gene sequences. Mycoscience 44:239–244.
Walters DR, Avrova A, Bingham IJ, Burnett FJ, Fountaine J, et al., 2012. Control of foliar diseases in barley: towards an integrated approach. European Journal of Plant Pathology 133: 33–73.
White TJ, Bruns TD, Lee SB, Taylor JW, 1990. Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Innis, M.A., Gelefand, D.H., Sninsky, J.J. and Sussman, A.S. (eds.). Academic Press. New York. Pp. 315–322.
Zhong S, Steffenson BJ, 2001. Virulence and molecular diversity in Cochliobolus sativus. Phytopathology 91: 469–476.
Journal of Applied Research in Plant Protection
Volume 11, Issue 1
February 2022
Pages 1-18

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  • Receive Date: 27 July 2021
  • Accept Date: 27 July 2021

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