Impact of Different Diets on Some Biological and Physiological Parameters in the Helicoverpa armigera (Hübner,1808) (Lepidoptera: Noctuidae)

Document Type : Research Paper

Authors

1 M.S. Student of Agricultural Entomology, Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

2 Professor, Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

3 Postdoctoral Researcher of Agricultural Entomology, Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

Abstract

Abstract
In current research, the effect of four artificial diets containing cowpea, red kidney bean, navy bean and corn seed flour on protein content of 4 th instars whole body; 5 thand 6 thinstars larval and pre-pupal gut and fat bodies; and pupal whole body of the cotton bollworm (Helicoverpa armigera Hübner), were investigated. Also, the effect of different diets on the digestive α-amylase and pectinase was studied. Protein patterns were also compared using SDS-PAGE. According to the results, the lowest amount of protein in 4 thinstars whole body and 5 thinstars gut and fat bodies was seen in corn as diet and highest one was in navy bean diet. Insects reared on the corn as diet did not developed to 6 th larval stage during the experiment. Except for the protein content of 6th instars fat bodies, no significant differences were observed in the other treatments of the 6th instars and pre-pupa. The lowest amount of pupal protein was found in red kidney bean diet and the highest one was in navy bean diet. Due to the decrease in protein content in different diets, insect weight and the activity of digestive α-amylase and pectinase were also affected and significant differences were observed. The least weight and activity of enzymes were recorded in corn diet. Diferences in the amount of protein was also detected in SDS-PAGE. Consequently, artificial diets of corn and red kidney bean are not suitable for this insect rearing and white bean and cowpea are reported as appropriate diets.
 

Keywords

References

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Journal of Applied Research in Plant Protection
Volume 9, Issue 2
September 2020
Pages 61-74

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  • Receive Date: 19 September 2020
  • Accept Date: 19 September 2020

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