تعیین قدرت بیمارگری Nucleopolyhedro virus روی کرم قوزه‌ی پنبه، Helicoverpa armigera و شب پره‌ی پشت الماسی، Plutella xylostella

نوع مقاله : مقاله پژوهشی

نویسندگان

1 مؤسسه‌ی تحقیقات گیاه پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران.

2 گروه گیاهپزشکی دانشکده کشاورزی دانشگاه شاهد تهران.

چکیده

چکیده
     ویروسNucleopolyhedrosis virus به‌خاطر مزیت­‌های فراوان مانند اثر اختصاصی روی لارو حشرات آفت، عدم تاثیر نامطلوب روی محیط زیست و جانداران غیرهدف و همچنین سهولت ترکیب و تلفیق با سایر روش­‌های کنترلی، جایگاه ویژه­ای در مدیریت کنترل آفات دارد. اولین قدم به‌عنوان پایه و اساس تحقیق روی این ویروس، بررسی قدرت بیمارگری آن درکنترل آفات مورد هدف است. در این تحقیق قدرت بیمارگری ویروس روی لارو سن دوم کرم قوزه‌ی پنبه،Helicoverpa armigera Hübner و شب پره­ی پشت الماسی،Plutella xylostella L. در دمای 2± 27 درجه­ی سلسیوس و رطوبت نسبی 5±65% و دوره‌ی نوری 16:8 (تاریکی:روشنایی) بررسی شد. غلظت کشنده­ی 50 درصد (LC50)  Nucleopolyhedrosis virus روی لارو سن دوم کرم قوزه‌ی پنبه Helicoverpa armigera  OB ml-1103×2/9 و شب پره‌ی پشت الماسی Plutella xylastella OB ml-1104×8/3 محاسبه شد، که بیمارگری ویروس با کمترین غلظت کشنده­ی 50 درصد روی کرم قوزه‌ی پنبه بهتربود. زمان لازم برای مرگ و میر 50 درصد (LT50) در غلظت OB ml-1106 روی لارو سن دوم،H. armigera  پنج روز و در جمعیت شب پره‌ی پشت الماسی، P. xylostella8/4 روز تعیین گردید که تقریبأ با هم مطابقت دارند. نتایج این تحقیق نشان داد که Nucleopolyhedro virus دارای قدرت بیمارگری بیشتری روی کرم قوزه‌ی پنبه نسبت به شب پره‌ی پشت الماسی است. به نظر می رسد اگرچه این ویروس روی میزبان اصلی قدرت بیمارگری بیشتری دارد ولی نتایج این مطالعه نشان داد  که  می­تواند بعنوان عامل کنترل شب پره‌ی پشت الماسی نیز بکار گرفته شود.

 

کلیدواژه‌ها


عنوان مقاله [English]

Virulence Determination Nuclear Polyhedrosis Virus on Cotton Bollworm Helicoverpa armigera and Diamond Back Moth, Plutella xylostella

نویسندگان [English]

  • Mariyam Kalantari 1
  • Zahra MagholiFard 2
  • Rasol Marzban 1
1 Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.
2 Deprartmetn of Plant Protection, Faculty of Agriculture, Shahed University, Tehran, Iran.
چکیده [English]

Abstract
Nucleopolyhedro virus (NPV) has an important role in pest management programs. Because of many advantages such as selective effect on larvae of insect pest, no undesirable effects on environment and non-target organisms and also compatibility with other pest management tactics. In this research, the virulence of a native isolate was evaluated on the larvae of cotton bollworm, Helicoverpa armigera Hübner and diamond back moth, Plutella xylostella at 27±2 oC, 65±5% RH and 16L: 8D photoperiod. Based on the results, 50% lethal concentration (LC50) of virus on the 2nd larval instars of H. armigera and P. xylostella were determined as 9.2×103 OB/ ml -1and 3.8×104 OB/ml -1, respectively. Also results (LC50 – values), showed that NPV was more virulent on H. armigera than on the P. xylostella. Values obtained for 50% lethal time (LT50) of NPV were almost similar 5 and 4.8 days post treatment for H. armigera and P. xylostella, respectively.

کلیدواژه‌ها [English]

  • Keywords: Microbial control
  • Helicoverpa armigera
  • Plutella xylostella
  • Virus
منابع
بهداد ا، (1376). آفات گیاهان زراعی ایران. انتشارات یادبود، اصفهان، 618 صفحه.
خواجه نوری ع، (1347). آمار پیشرفته و بیومتری، چاپ اول، انتشارات دانشگاه تهران،567 صفحه.
مرزبان ر و بنی عامری و. (1383). بررسی تأثیر حشره کشهای  بیولوژیکی و شیمیایی در کنترل شب پره پشت الماسی کلم، Plutella xylostella (Lep.: Plutellidae). خلاصه مقالات شانزدهمین کنگره گیاهپزشکی ایران، صفحه 188.
مرزبان ر و عسکری ح .(1388). دستاوردها، چالش ها و چشم انداز کنترل میکروبی آفات. همایش ملی نیم قرن مصرف آفتکش ها. موسسه تحقیقات گیاهپزشکی کشور.
An SH, Wang D, Yang Z.N, Guo ZJ, Xu HJ, Sun JX and Zhang CX, 2005. Characterization of a late expression gene, open reading frame 128 of Helicoverpa armigera single-nucleocapsid NucleoPolyhedroVirus. Journal Archives of Virology 150: 2453-2466.
Biever KD and Andrew PL, 1984. Susceptibility of Lepidopterous larvae to Plutella xylostella nuclear polyhedrosis virus. Journal of Invertebrate Pathology 44: 117-119.
Bird LJ and Akhurst RJ, 2007. Variation in susceptibility of Helicoverpa armigera (Hubner) and Helicoverpa Punctigera (Wallengren) in Australia to two Bacillus thuringiensis toxins. Journal of Invertebrate Pathology 94: 84-94.                                    
Caballera P, Zuidema O, Santiago-Alvarez Cand Vlak, MJ, 1992. Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus. Journal Biocontrol Science and Technology 2(2): 145-157.
Dezianian A, Sajap AS, Lau WH, Omar D, Kadir HA, Mohzmed R and Yusoh MRM, 2010. Morphological Characteristics of P. xylostella Granulovirus and Effects on Its Larval Host Diamondback Moth, Plutella xylostella L. (Lepidoptera: Plutellidae). American journal of Agricultural and Biological Sciences 5(1): 43-49.
Entwistle PF, 1983. Control of insects by virus diseases. Biocontrol News and Information, 4(3): 203-229
Evans HF and Shapiro M, 1981. Viruses. In: Lacey, L. (Ed.), Manual of Techniques in Insect Pathology. Academic Press London. pp. 17–54.A
Fahimi A, Kharrazi-Pakdel A and Talaei-Hassanloui R, 2008. Evalution of Effect of PxGV-Taiwanii on Cabbage moth, Plutella xylostella (Lep.: Plutellidae) in Laboratory Conditions. Pakistan Journal of Biological Sciences 11(13): 1768-1770.
Farrar RR, Shapiro M and Shepard M, 2007. Relative activity of baculoviruses of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae). BioControl, 52: 657-667.
Fitt G P, 1989. The ecology of Heliothis species in relation to agroecosystems. Annual. Review of Entomology, 34: 17-52.
Grzywacz D, Rossbach A, Rauf A, Russell DA, Srinivasan R and Shelton AM, 2009. Current control methods for diamondback moth and other brassica insect pests and the prospects for improved management with lepidopteran-resistant Bt vegetable brassica in Asia and Africa. Crop Protection 29(1): 68-79.
Guo ZJ, An SH, Wang D, Liu YH, Kumar VS and Zhang CX, 2005. Characterization of Ha29, a specific gene for Helicoverpa armigera single-nucleocapsid NucleoPolyhedroVirus. Journal Biochemistry Molecular Biology 38: 354-359
Hong-Lian Sh, Du-JuanD, Jin-Dong H, Jin-xin W and Xiao-Fan Z,2008. Construction of the recombinant baculovirus AcMNPV with cathepsin B-Like proteinase and its insecticidal activity against Helicoverpa armigera. Pesticide Biochemistry and physiology 91:141-146.
Hughes PR, Gettig RR, McCarthy WJ, 1983. Comparison of the time- Mortality response of Heliothis zea to 14 isolates of Heliothis nuclear polyhedrosis virus. Journal Invertebrate Pathology 41: 256-261.
Jaques RP, 1972.Control of the cabbage looper and the imported cabbage worm by virus and bacterial. Journal of Economic Entomology 65: 757-760
Jehle JA, Blissard GW, Bonning BC, Cory JS, HerniousEA, Rohrmann GF, Theilmann DA, Thiem SM and Vlak JM,2006. On the classification and nomenclature of baculoviruses: A proposal for revision. Archives of Virology 151: 1257-1266.
Kadir HBA, Payne CC, Crook NE, Fenlon JS and Winstanely D, 1999. The comparative susceptibility of the diamondback moth, Plutella xylostella and some other major Lepidoptera pests of brassica crops to a range of baculovirus. Biocontrol Science and Technology 9: 421-433.
Kalantari M, Marzban R and Imani S, 2013. Effect of Bacillus thuringiensis isolates and single nuclear polyhedrosis virus in combination and alone on Helicoverpa armigera. Archives of Phytopathology and Plant Protection
Kumar NS, Murugan K and Zhang W, 2008. Additive interaction of Helicoverpa armigera Nucleopolyhedrovirus and Azadirachtin. BioControl 53: 869-880.
Lehane MJ, 1997. Peritrophic matrix structure and function. Annual Review of Entomology, 42: 525-550.
Long G, Chen X, PetersD, Vlak JM and Hu Z, 2003. Open reading frame 122 of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions. Journal of General Virology 84: 115-121.
Lange M, Wang H, Zhihong H and Jehle JA, 2004. Towards a molecular identification and classification system of lepidopteran-specific baculoviruses. Virology, 325: 36-47.
Magholi Z, Abbasipour H and Marzban R, 2014. Effects of Helicoverpa armigera Nucleopolyhedrosis virus (HaNPV) on the larvae of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae).  Journal Plant Protectection Science 50(4): 184–189.
Marzban R, He Q, Zhang QW and Liu XX, 2013. Histopathology of Cotton bollworm midgut infected with Helicoverpa armigera Cytoplasmic polyhedrosis virus. Brazili. Journal Microbiology 44(4): 1231-1236.
Marzban R. 2012. Midgut pH profile and energy differences in lipid, protein and glycogen metabolism of Bacillus thuringiensis Cry1Ac toxin and Cypovirus-infected Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). Journalof theEntomological Research Society 14: 45-53.
Mitter C and Mattews M, 1993. Biosystematic of the Heliothinae (Lepidoptera: Noctuidae). Annual Review of Entomology 38: 207-225.
Navon A, 2000. Control of lepidopteran pests with Bacillus thuringiensis. PP.125-146. In: Entwistle, P.F., Cory, J.S., Bailey, M.J., Higgs, S. (Eds), Bacillus thuringiensis, an Environmental Biopesticide: Theory and practice. Wiley New York USA.
Padmavathamma K and Veeresh GK, 1991. Effect of Larval age and dosage of nuclear polyhedrosis virus on the susceptibility of diamondback moth, Plutella xylostella. Entomologia Experimentalis et Applicata 60: 39-42.
Rabindra RJ, Geethea N, RenukaS, Varadhaarajan S and Regupatholy A, 1997. Occurence of a granulosis virus from two populations of Plutella xylostella in India. Proceedings of the 3rd International Work Shop on Management of Diamondback Moth and other Crucifer pests. Kuala Lumpur, Malaysia, 29 Oct.-1Nov. 1996 pp.113-115.
Shelton AM, 2004. Management of diamondback moth. Pp.47-53. In: Sivapragasam, A, Loke, W.H., A.K. Hussan and G.S. Lim (Eds.) The Management of Diamondback Moth and other Crucifer pests: Proceedings of the Third International Workshop of Diamondback Moth. Kualalumpur Malaysia.
Talekar NS and Shelton AM, 1993. Biology, ecology and management of the diamondback moth, Annual Review of Entomology 38: 275-301.
Teakle, RE, and Byrne VS, 1989. Nuclearpolyhedrosis virus production in Heliothis armigera infected at different larval ages. Journal of Invertebrate Pathology 53: 21-24.
Trang T and Chaudhari S, 2002. Bioassay of nuclear polyhedrosis virus (NPV) and in combination with insecticide on Spodoptera litura (Fab). Omonrice 10: 45-53.
Treacy MF, All JN and Kukel CR, 1997. Invertebrate selectivity of a recombinant baculovirus: Case study on AaHIT gene-inserted Autographa Califera nuclear polyhedrosis virus. Pp.57-68 In: (Bondari, K. (ed.). New Developments in Entomology Research Signpost London.
Verkerk RHJ and Wright DJ, 1996. Multitropic interactions and management of the diamondback moth, a review. Bulletin of Entomological Research 86: 205-216.
Wang XP, Fang YL and Zhang ZN, 2005. Effect of male and female multiple mating on the fecundity, fertility and longevity of diamondback moth, Plutella xylostella (L.). Blackwell Verlag, Berlin, Germany 129(1): 39-42.
Wright D, 2004. Biological control of DBM: a global perspective. Pp.9-14. In: Bordat D and Kirk A A (eds.), Improving Biocontrol of Plutella xylostella. CIRAD, Montpelier, France (2004), Proceedings of the International symposium in Montpelier France 21-24 Oct 2002.
Zhang GY, 1994. Research, development and application of Heliothis viral pesticide in China. Resources and Environment in the Yangtze Basin 3:36–41. (In Chinese)