نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز
2 استادیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران؛
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Mountains of Shahiyoon and Salandkooh (Dezful, Khuzestan), Kabirkooh (Abdanan, Ilam) and ZazoMahroo (Aligoodarz, Lorestan) constitute small parts of the Zagrosian forests in Iran. During 2020-2022, 50 symptomatic samples from forest trees including Acer sp., Amygdalus scoparia, Crataegus sp., Pistacia atlantica, Quercus brantii and Ziziphus sp., showing leaf blight, leaf spot, fruit rot, decline and dieback, were collected. Totally, 153 fungal isolates were obtained on potato–dextrose–agar medium (PDA), of which 17 were identified at species level based on combination of morphological characteristics and sequence data of marker genes. Morphological characteristics and growth rate were evaluated on oat -meal - agar (OA) and PDA. The ITS-rDNA region (in some isolates) and part of the tub2 (in all isolates) and rpb2 (in some isolates) genes were amplified and sequenced. The obtained sequences were compared using BLASTn search algorithm in NCBI database and their phylogenetic analyses were performed based on single or multiple genomic regions. Accordingly, the isolates were identified as follow: Chaetomium anastomosans, C. ascotrichoides, C. subglobosum, Didymella pomorum, D. prolaticolla, and Paramicrosphaeropsis iranica. Based on literature, this is the first record of C. anastomosans, C. subglobosum and D. prolaticolla for the mycobiota of Iran. In addition, new hosts for C. anastomosans (P. atlantica, Q. brantii and Crataegus sp.), C. ascotrichoides (Acer sp.), C. subglobosum (Q. brantii) and P. iranica (A. scoparia and P. atlantica) are reported.
کلیدواژهها [English]