تاثیر افزودن آنتی بیوتیک و پروبیوتیک به رژیم غذایی روی فراسنجه های زیستی کرم زرد آرد Tenebrio molitor

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه خلیج فارس، بوشهر، ایران.

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه خلیج فارس، بوشهر، ایران.

چکیده

چکیده
کرم زرد آرد، Tenebrio molitor، یک منبع بسیار غنی از پروتئین و دیگر مواد غذایی است. در این تحقیق به منظور بهبود پرورش این حشره، برخی فراسنجه­ های زیستی آن با تغذیه از شانزده رژیم غذایی حاوی دزهای مختلف آنتی­ بیوتیک لینکومایسین (صفر، 005/0، 01/0 و 02/0 درصد) و سه نوع پروبیوتیک (فاقد پروبیوتیک (p0)، پروبیوتیک تجاری (pA)، پروبیوتیک تهیه شده از کشت لاکتوباسیلوس­ها (pB) و پروبیوتیک حاوی مخمر تجاری (pC)) بررسی شدند. هر سه پروبیوتیک به مقدار یک درصد با غلظتCFU/gr  108 در رژیم غذایی مورد استفاده قرار گرفتند. تیمارهای آزمایشی روی لاروهای سن اول حشره در رژیم غذایی مبتنی بر سبوس و هویج اعمال شدند. بر اساس نتایج، در تیمار pC-دز 01/0 درصد با میانگین 16/1 ± 75/67 روز، افزایش معنی­ داری در طول دوره نشوونمای لاروی نسبت به شاهد (04/1 ± 17/64 روز) مشاهده شد. بیشترین میانگین وزن لاروی، 30/5 ± 17/144 میلی­گرم، مربوط به تیمار pA-دز 01/0 درصد بود که تنها با میانگین تیمار pC-دز 01/0 درصد (05/3 ± 50/117 میلی­گرم) دارای اختلاف معنی­ دار بود. درصد شفیرگی در دز صفر (77/1 ± 46/84 درصد) به طور معنی­ داری نسبت به دزهای 01/0 و 005/0 درصد آنتی ­بیوتیک (به ترتیب 8 و 32/9 درصد) کمتر بود. صفات طول دوره نشو و نمای شفیرگی، وزن شفیره ­ها، و وزن و درصد ظهور حشرات کامل تحت تاثیر رژیم ­های غذایی قرار نگرفتند. بر اساس یافته ­های این تحقیق، برهمکنش آنتی­ بیوتیک و پروبیوتیک می­ تواند برخی فراسنجه­ های رشدی و نشو و نمایی کرم زرد آرد را تحت تاثیر قرار دهد که در برنامه­ های پرورش حشره می­تواند مد نظر قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of dietary supplementation with antibiotic and probiotic on Biological Parameters of Yellow mealworm, Tenebrio molitor

نویسندگان [English]

  • Fariba Sohrabi 1
  • Hassan Habibi 2
  • Salim Moramazi 2
1 Department of Plant Protection, Faculty of Agriculture, Persian Gulf University, Bushehr, Iran.
2 Department of Animal Sciences, Faculty of Agriculture, Persian Gulf University, Bushehr, Iran.
چکیده [English]

Abstract
Yellow mealworm, Tenebrio molitor, is a wealthy source of proteins and other nutrients. In this study, to improve the breeding of this edible insect, some of its biological parameters were evaluated by feeding on sixteen diets containing different levels of the antibiotic lincomycin (doses of 0, 0.005, 0.01 and 0.02%) and probiotics. Probiotic levels included: 1-probiotic-free (p0), 2- commercial probiotic (pA), 3- probiotic prepared from culture of Lactobacillus bacteria (pB), and 4- probiotic containing commercial yeast (pC). All three probiotics were used at a dose rate of 1% (108 CFU/gr). Treatments were applied to first instar larvae in bran and carrot-based diet. Based on the results, a significant increase was observed in the larval life span in the treatment of pC-dose of 0.01%, with an average of 67.75 ± 1.16 days, compared to the control (64.17 ± 1.04 days). The greatest mean larval weight, 144.17 ± 5.30 mg, was related to the pA-dose of 0.01%, which was significantly different only with pC-dose of 0.01% (117.50 ± 3.05 mg). The pupation percentage at zero dose (84.46 ± 1.77%) was significantly lower than the doses of 0.01 and 0.005% of antibiotics (8 and 9.32%, respectively). Traits of pupal life span, pupal weight, adult weight and adult emergence rate were not affected by diets. Based on the findings, the interaction of antibiotics and probiotics can affect some growth and developmental parameters of the yellow mealworm that can be considered in insect breeding programs.

کلیدواژه‌ها [English]

  • Keywords: Bacteria
  • Diet
  • Lincomycin
  • Mass Rearing
  • Yellow Mealworm
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