بررسی سطوح نسبی مقاومت به شانکر باکتریایی در ژرم پلاسم ایرانی آلبالو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه بیماری شناسی گیاهی، دانشگاه ازاد اسلامی، واحد تاکستان.

2 پژوهشکده میوه های معتدله و سردسیری، موسسه تحقیقات باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج.

چکیده

چکیده
بیماری شانکر باکتریایی با عامل Pseudomons syringae pv. syringae یکی از بیماری ­های مهم درختان میوه هسته دار از جمله  آلبالو است. در این تحقیق، سطوح نسبی حساسیت برخی از ژنوتیپ­ های بومی آلبالو به این بیماری در شرایط باغی بررسی شد. ارزیابی­ ها در شرایط باغی بر روی 46 ژنوتیپ در مرحله درخت بالغ در دو سال متوالی (1397 و 1398) و 18 ژنوتیپ در مرحله نهال در سال 1399 انجام شد. مایه تلقیح از مخلوط سه جدایه ایرانی باکتری عامل تهیه شد. نهال­ های در پائیز 1398 مایه ­زنی شده و یک سال بعد طول شانکر اندازه­ گیری شد. درختان بالغ در پائیز 1396 مایه­ زنی شده و یک و دو سال بعد طول شانکر اندازه­ گیری شد. بر اساس نتایج، میانگین­ طول شانکر در هر دو مرحله بلوغ و نهال در ژنوتیپ­ های مختلف متفاوت بود. در نهال­ ها، بیشترین و کم­ترین طول شانکر به ­ترتیب متعلق به ژنوتیپ ­های 230 و 125بود. براساس نتایج تجزیه مرکب داده­ های دوساله درختان بالغ، بیشترین و کم­ترین طول شانکر به ­ترتیب مربوط به ژنوتیپ ­های 208 و 104 بود و بنابراین ژنوتیپ­ های فوق به­ عنوان حساس­ ترین و مقاوم ­ترین گروه­ بندی شدند. بین طول شانکر در نهال و درخت بالغ ارتباطی دیده نشد. بر اساس نتایج حاصله در درختان بالغ، کشت ژنوتیپ ­های حساسی مانند 108، 206 و 220 در مناطق مستعد این بیماری توصیه نمی ­شود.
 

کلیدواژه‌ها


عنوان مقاله [English]

Determination of bacterial canker resistance level in Iranian sour cherry germplasm

نویسندگان [English]

  • Shahab Ranjbari 1
  • Mansureh Keshavarzi 2
  • Naser Bouzai 2
  • Seyedeh Nikoo Kakovan 1
  • Zeynab salehi 2
1 Department of Plant Pathology, Islamic Azad University, Takestan Branch, Iran.
2 Temperate Fruit Center, Horticultural Research Institute, AREEO, Karaj, Iran.
چکیده [English]

Abstract
Bacterial canker disease caused by Pseudomons syringae pv. syringae, is one of the most damaging diseases in sour cherries. In this study, relative resistance of some local sour cherries to this disease was evaluated. Totally, 46 genotypes in adult stage in two repetitive years (2018 and 2019) and 18 genotypes in seedling stage at year 2020 were examined. A mixture of three local P. s. pv. syringae isolates was used as inoculum. Saplings trunks were inoculated in autumn 2019 and canker length was measured one year later. Adult trees were inoculated in autumn 2017 and canker length was recorded one and two years later. Based on result, mean canker length was different among genotypes in both stages. In saplings, the most and the least canker length were rated for genotypes 230 and 125, respectively. Compound analysis of adult trees data showed the least and the most canker lengths were related to genotypes 208 and 104, respectively which were thus rated as the most susceptible and the most resistant genotypes. Based on the results obtained from adult plants, planting of susceptible genotypes like 108, 206 and 220 in risk area is not recommended.
 
 

کلیدواژه‌ها [English]

  • Keywords: bacterial canker
  • resistance
  • Pseudomonas syringae
  • sour cherry
References
 
Abe K, Kotoda N, Kato H, Soejima J, 2007. Resistance sources to Valsa canker (Valsa ceratosperma) in a germplasm collection of diverse Malus species. Plant Breeding 126: 449–453.
Anonymous, 2018. Food and Agriculture Organization of the United Nations. FAO Statistical Databases.http://www.fao.org/.
Arny DC, Lindow SE, Upper CD, 1976. Frost sensitivity of Zea mays increased by application of Pseudomonas syringae. Nature 262: 282–284.
Babaali A, Keshavarzi M, Bouzari N, Shakin AM, Hosseinova S, 2013. Relative Resistance of some Local and Commercial Cherry Genotypes to Pseudomonas syringae. Seed and Plant Journal 29: 295-310 (In Persian with English abstract).
Bahar M, Mogahedi H, Akhyanii A, 1981. Apricot bacterial canker in Isfahan. Iranian Journal of Plant Pathology 18: 58-68 (In Persian with English abstract).
Balan V, Oprea M, Drosu S, Chireceanu C, Tudor V, et al.,2006. Maintenance of biodiversity of apricot tree phenotypes in Romania. Acta Horticulturae 701: 199–206.
Balaž J, Ilicˇic R, Ognjanov V, Ivanovic Z, Popovic T, 2016. Etiology of bacterial canker on young sweet cherry trees in Serbia. Plant Pathology 98: 285–294.
Banapour A, Zakeri Z, Amani G, 1990. Cherry bacterial canker in Tehran. Proceedings of the 9th Iranian Plant Protection Congress, Mashhad, Iran. P. 94 (In Persian with English abstract).
Bouzari N, Ganji A, Karami F, Ghasemi A, Zarinbal M, et al., 2010. Evaluation of local sour cherry germplasm to obtain appropriate cultivars and rootstocks. Research final report 89.1532. AREEO, Tehran, Iran (In Persian with English abstract).
Bouzari, N. 2006. Evaluation of cherry cultivars for bacterial canker resistance. Proceedings of the 17th Iranian Plant Protection Congress, Karaj. P. 376 (In Persian with English abstract).
Cao T, Sayler RJ, DeJong TM, Kirkpatrick BC, Bostock RM, et al., 1999. Influence of stem diameter, water content, and freezing-thawing on bacterial canker development in excised stems of dormant stone fruit. Phytopathology 89: 962–966.
Christensen JV, 1986. Evaluation of characteristics of 18 sour cherry cultivars. Danish Research Service for Plant and Soil Science. Report 1864. Tidsskrift for Planteavl 90: 339–347.
Crosse JE, Garrett CME, 1966. Bacterial canker of stone-fruits. VII. Infection experiments with Pseudomonas morsprunorum and P. syringae. Annals of Applied Biology 58: 31–41
Dowler WM, Weaver DJ, 1975. Isolation and characterization of fluorescent pseudomonads from apparently healthy peach trees. Phytopathology 65: 233–236.
Erfaninik M, Rezaee R, Charegani H, 2018. Identification of the causal agent of bacterial canker of stone fruit trees Pseudomonas syringae pv. syringae in Kohgiluyeh and Boyer-Ahmad province and comparison of some cherry cultivars resistance to it. Journal of Applied Research in Plant Protection 7: 31–44 (In Persian with English abstract).
Farhadfar S, Keshavarzi M, Bouzari N, Ladan Moghadam A, Soleimani A, 2016. Susceptibility of cherries to bacterial canker in field and laboratory. International Journal of Agriculture and Forestry 6: 20–27.
Fischer M, Hohlfed B, 1998. Resistance tests in sweet cherries. Acta Horticulture 468: 87–94.
Fuchs A, De Vries DP, 1964. Optreden, bestrijding en voorkomen van bacteria kanker. Kersen, Meded Dir Tuinb 27: 546–56.
Jafarpour B, 1993. Resistant cultivars of apricot to bacterial canker (Pseudomonas syringae pv. syringae) in Mashhad.  Current Plant Science and Biotechnology in Agriculture 18: 327–332.
Garrett CME, 1979. Screening Prunus rootstocks for resistance to bacterial canker caused by Pseudomonas morsprunorum. Journal of Horticultural Sciences 54: 189–193.
Hattingh MJ, Roos IMM, 1995. Bacterial canker. In: Ogawa JM. & Zehr EI (ed). In: Compendum of Stone Fruit Disease. APS Press, Pp. 48–50.
Hulin T, Jackson RW, Harrison RS, Mansfield JW, 2020. Cherry picking by pseudomonads: After a century of research on canker, genomics provides insights into the evolution of pathogenicity towards stone fruits. Plant Pathology 69: 962–978.
Keshavarzi M, Bouzari N, 2014. Resistance to bacterial canker in a number of selected apricot genotypes. Research final report 46359, AREEO, Tehran, Iran (In Persian with English abstract). 
Keshavarzi M, Dejampour J, 2018. Evaluation of bacterial canker resistance in a number of apricot hybrids. Research final report 43320, AREEO, Tehran, Iran (In Persian with English abstract). 
Kesner CD, 1999. Mechanical summer tiping of tart cherries. http://www.Goodfruit grower.
Klement Z, Rozsnyay DS, Balo E, Prileszky M, 1984. The effect of cold on development of bacterial canker in apricot trees infected with Pseudomonas syringae pv. syringae. Physiological Plant Pathology 24: 237–246
Latorre BA, Lillo C, Rioja ME, 2002. Effect of temperature, free moisture duration and inoculum concentration on infection of sweet cherry by Pseudomonas syringe. pv. syringae.Phytoparasitica 30: 410–419.
Moore LW, Pscheidt JW, 2010. Diseases caused by Pseudomonas syringae. Pacific Northwest Plant Disease Management Handbook. https://pnwhandbooks.org/node/408.
Okie WR, Ramming DW, 1999. Plum breeding worldwide. Horticultural Technology 9: 162–176.
Prunier J-P, Psallidas P, Scortichini M, Simeone AM, Martins JMS, et al., 1999. European co-operative research on apricot bacterial diseases. Acta Horticulturae 488: 699–704.
Rioux D, 1996. Compartmentalization in trees: new findings during the study of Dutch elm disease. In: Nicole M and Gianinazzi-Pearson V (eds) Histology, Ultrastructure and Molecular cytology of Plant-Microorganism Interactions. Berlin: Springer, The Netherlands. Pp. 211–225.
Rudolph K, Burr TJ, Mansfield JW, Stead D, Vivian A, et al., 1997. Pseudomonas syringae Pathovars and Related Pathogens. Kluwer Academic Publishers. P. 9.
Santi F, Russell K, Menard M, Dufour J, 2004.  Screening wild cherry for resistance to bacterial canker by laboratory and field tests. Forest Pathology 34: 349–362.
Shamsbakhsh M, Rahimian H, 1989. Characterization of stone fruits bacterial canker in Mazandaran. Proceedings of the 9th Iranian Plant Protection Congress, Mashhad. P. 134 (In Persian with English abstract).
Spotts RA, Wallis KM, Serdani M, Azarenko AN, 2010. Bacterial canker of sweet cherry in Oregon, Infection of horticultural and natural wounds and resistance of cultivar and rootstock combination. Plant Disease 94: 345–350.
Sule S, Seemuller , 1987. The role of ice formation of sour cherry leaves by Pseudomonas syringae pv. syringae. Phytopathology 77: 173–177.
Topp BL, Heaton JB, Russell DM, 1989. Introduction and evaluation of stone fruit varieties for the Granit belt of Queensland. Acta Horticulture 240: 39–42.