استفاده از روش های سری زمانی جهت پیش بینی بیان ژن های دفاعی گندم تلقیح شده با Zymoseptoria tritici و اسیدسالیسیلیک

نویسندگان

1 استادیار دانشکده کشاورزی و منابع طبیعی، دانشگاه اردکان، اردکان، ایران.

2 استادیار پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران.

3 استادیاردانشکده کشاورزی و منابع طبیعی، دانشگاه اردکان، اردکان، ایران.

چکیده

چکیده
اندازه­گیری میزان بیان ژن­های دفاعی گیاهی روشی پرکاربرد و مفید برای بررسی مکانیسم مقاومت در گیاهان است، اما این روش در عین حال هزینه بر و زمان­بر نیز می­باشد. به منظور بررسی تأثیر اسیدسالیسیلیک بر تغییرات بیان بعضی از ژن­های دفاعی درگیر در مقاومت (NADPH اکسیداز، اگزالات اکسیداز، پروتئین Stress responsive، گلوتاتیون S ترانسفراز و سوپراکسید دیسموتاز) گندم­های تلقیح شده با بیمارگر  Z. triticiبه روش نوردرن­بلات معکوس بررسی شد. بر اساس اطلاعات به دست آمده از نوردرن بلات معکوس جهت پیش­بینی بیان ژن­های درگیر در مقاومت از روش­های میانگین متحرک ساده، میانگین متحرک وزنی و هموار سازی نمایی استفاده شد. در  24 ساعت بعد از تلقیح بیمارگر در همۀ ژن­های مورد بررسی و در هر دو تیمار میزان بیان به بیشترین مقدار خود رسید و علی­رغم تفاوت در هر ژن روند کلی افزایشی بود. نتایج این مطالعه نشان داد که استفاده از روش هموارسازی نمایی، با توجه به محاسبۀ و مقایسۀ شاخص‏های قدرت پیش­بینی، دارای دقت بالاتری نسبت به دو روش دیگر می­باشد. میزان بیان، در مورد سه ژن NADPH اکسیداز، گلوتاتیون S ترانسفراز و اگزالات اکسیداز روند مشابه داشتند، به عبارتی تا 24 ساعت بعد از تلقیح بیمارگر، اسیدسالیسیلیک باعث افزایش شد و سپس میزان بیان روند کاهشی در پیش گرفت. بررسی بیان ژن روش بسیار حساسی است، و باید تحت شرایط کنترل شده باشد، و فقط تیمار مورد نظر محقق، تأثیرگذار بر روی بیان ژن در گیاه باشد، در این شرایط، پیشنهاد می­شود اطلاعات بیان ژن­ها در طی دوره­هایی ابتدایی اندازه­گیری شود، سپس با استفاده از این اطلاعات و روش­های پیش‏بینی میزان بیان، برای زمان­های بعدی مورد پیش­بینی قرار گیرد.
 

کلیدواژه‌ها

عنوان مقاله [English]

Application of Time Series Methods to Predict the Process of Expression of Defense Genes in Wheat During Infection whit Zymoseptoria tritici

نویسندگان [English]

  • Jalal Gholamnejad 1
  • Forogh Sanjarian 2
  • Masoud Fehresti 3
1 Department of Horticultural Sciences, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, 8951895491, Iran
2 Assistant Professor, National Institute of Genetic Engineering and Biotechnology
3 Department of Horticultural Sciences, Faculty of Agriculture & Natural Resources, Ardakan University, P.O. Box 184, Ardakan, 8951895491, Iran
چکیده [English]

Abstract
The measurement of the level of defense genes expression is a useful method for investigating the resistance mechanisms of plants, but it is also time consuming and costly. In this study, it was investigated the effect of salicylic acid on the defense genes expression involved in the resistance of wheat inoculated with Z. tritici, by reverse northern blot method. In this research, the amount of genes expression was measured including NADPH oxidase, oxalate oxidase, stress responsive protein and glutathione S transferase superoxide dismutase was by reverse northern Blot method. In the next step, based on the information of reverse northern Blot method, it was predicted the rate of genes expression using prediction methods including simple moving average, weighted moving average and single exponential smoothing. The highest genes expression was observed at 24 hours after pathogen inoculation, in both treatments; however, there were differences for each gene, but the overall trend was incremental. The results of this study showed that the use of single exponential smoothing method, with regard to calculating and comparing predictive power indexes, has a higher accuracy than two simple and weighted moving averages. The expression of NADPH oxidase, glutathione S transferase and oxalate oxidase genes showed a similar trend, however salicylic acid increased the genes expression to 24 hours after inoculation, and then the rate of expression decreased. Examining gene expression is a very sensitive method, and should be under controlled conditions, and should be the only treatment the researcher can influence on gene expression in the plant. Then, using this information and methods of predicting the expression, predict the next time.
 
 

کلیدواژه‌ها [English]

  • Keywords: Gene expression
  • Reverse northern blot
  • Prediction
  • Wheat
  • Zymoseptoria tritici

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پژوهش های کاربردی در گیاهپزشکی
دوره 8، شماره 4
اسفند 1398
صفحه 81-101

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  • تاریخ دریافت: 21 اسفند 1398
  • تاریخ پذیرش: 21 اسفند 1398

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